E3 ubiquitin/ISG15 ligase TRIM25 (TRIM25) is a 630-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: Q14258.
Explore in 3D Color by confidence AlphaFold DB UniProt
The mean pLDDT of this model is 84.1 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 67% |
| 70 to 90 | Confident: backbone generally right | 16% |
| 50 to 70 | Low: treat with caution | 3% |
| Below 50 | Very low: often disordered regions | 15% |
What pLDDT means and how to read it
Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase (PubMed:16352599). Involved in innate immune defense against viruses by mediating ubiquitination of RIGI and IFIH1 (PubMed:17392790, PubMed:29357390, PubMed:30193849, PubMed:31710640, PubMed:33849980, PubMed:36045682, PubMed:40719442). Mediates 'Lys-63'-linked polyubiquitination of the RIGI N-terminal CARD-like region and may play a role in signal transduction that leads to the production of interferons in response to viral infection (PubMed:17392790, PubMed:23950712). Mediates 'Lys-63'-linked polyubiquitination of IFIH1 (PubMed:30193849). Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation…
Forms homodimers (PubMed:27154206, PubMed:27425606, PubMed:29357390, PubMed:31710640). Interacts (via SPRY domain) with RIGI (via CARD domain). Interacts with ZFHX3. Interacts with NLRP12; this interaction reduces the E3 ubiquitin ligase TRIM25-mediated 'Lys-63'-linked RIGI activation. Interacts with the KHDC3L/FILIA-OOEP/FLOPED scaffold complex and BLM at DNA replication forks (By similarity).…
Cytoplasm, Cytoplasm, Stress granule, Nucleus
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 9I0T | X-ray | 1.8 Å | A=435-630 |
| 6FLM | X-ray | 2.01 Å | A/B/C=435-630 |
| 5FER | X-ray | 2.34 Å | A/D=1-82 |
| 5EYA | X-ray | 2.4 Å | F/G=1-83 |
| 4LTB | X-ray | 2.59 Å | A/B=189-379 |
| 9IUN | X-ray | 2.7 Å | A/B/C/D/E/F/G/H/I/J/K/L=433-630 |
| 4CFG | X-ray | 2.8 Å | A/B=1-630 |
| 5NT1 | X-ray | 2.82 Å | A/E/I=190-379 |
| 6FLN | X-ray | 3.6 Å | A/B/E=189-630 |
| 5NT2 | X-ray | 4.26 Å | A/I/N/V=190-379 |
MolViewer loads the AlphaFold model straight from AlphaFold DB into your browser. Show it as a cartoon, color by pLDDT, measure distances and angles, and load a PDB structure next to it to compare.