AP2-associated protein kinase 1 (AAK1) is a 961-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: Q2M2I8.
Explore in 3D Color by confidence AlphaFold DB UniProt
The mean pLDDT of this model is 58.4 (low overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 31% |
| 70 to 90 | Confident: backbone generally right | 5% |
| 50 to 70 | Low: treat with caution | 3% |
| Below 50 | Very low: often disordered regions | 61% |
What pLDDT means and how to read it
Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis (PubMed:11877457, PubMed:11877461, PubMed:12952931, PubMed:14617351, PubMed:17494869, PubMed:25653444). Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1 (PubMed:17494869). Preferentially, may phosphorylate substrates on threonine residues (PubMed:11877457, PubMed:18657069). Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes (PubMed:12952931).…
Interacts (via CBD domain) with clathrin (PubMed:14617351, PubMed:17494869). Interacts with AP-2 complex (PubMed:12952931, PubMed:14617351, PubMed:17494869). Interacts with NUMB (PubMed:18657069). Interacts with alpha-adaptin (By similarity). Interacts with EPS15 isoform 2 (PubMed:21464124). Interacts with membrane-bound activated NOTCH1 but not with the inactive full-length form of NOTCH1…
Cell membrane, Membrane, clathrin-coated pit, Presynapse
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 9F6S | X-ray | 1.0 Å | B=956-961 |
| 9F8T | X-ray | 1.71 Å | D/E=956-961 |
| 5TE0 | X-ray | 1.9 Å | A=27-365 |
| 4WSQ | X-ray | 1.95 Å | A/B=29-345 |
| 5L4Q | X-ray | 1.97 Å | A/B=27-365 |
| 9QB5 | X-ray | 2.0 Å | A/B=29-345 |
| 8GMD | X-ray | 2.2 Å | A/B=26-330 |
| 8GMC | X-ray | 2.5 Å | A/B=26-330 |
MolViewer loads the AlphaFold model straight from AlphaFold DB into your browser. Show it as a cartoon, color by pLDDT, measure distances and angles, and load a PDB structure next to it to compare.