Twinfilin-1 (Twf1) is a 350-residue protein from Mus musculus. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: Q91YR1.
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The mean pLDDT of this model is 91.4 (very high overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 81% |
| 70 to 90 | Confident: backbone generally right | 14% |
| 50 to 70 | Low: treat with caution | 4% |
| Below 50 | Very low: often disordered regions | 1% |
What pLDDT means and how to read it
Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles
Interacts with G-actin; ADP-actin form and capping protein (CP) (PubMed:12807912, PubMed:15282541, PubMed:16511569). May also be able to interact with TWF2 and phosphoinositides, PI(4,5)P2 (PubMed:12807912). When bound to PI(4,5)P2, it is down-regulated (PubMed:12807912). Interacts with ACTG1 (By similarity)
Cytoplasm, Cytoplasm, cytoskeleton
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 1M4J | X-ray | 1.6 Å | A/B=1-142 |
| 7DS6 | X-ray | 1.69 Å | C=315-327 |
| 7DS4 | X-ray | 1.85 Å | C=315-344 |
| 6RSW | X-ray | 1.95 Å | B=176-316 |
| 7DS2 | X-ray | 1.95 Å | C=315-350 |
| 7DS8 | X-ray | 1.95 Å | C=328-344 |
| 7DSB | X-ray | 2.44 Å | D=315-344 |
| 3DAW | X-ray | 2.55 Å | B=167-322 |
| 6YP9 | X-ray | 2.56 Å | B=176-315 |
| 2D8B | NMR | A=161-313 | |
| 2HD7 | NMR | A=176-316 |
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