Ubiquitin-like modifier-activating enzyme 5 (UBA5) is a 404-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: Q9GZZ9.
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The mean pLDDT of this model is 86.8 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 67% |
| 70 to 90 | Confident: backbone generally right | 15% |
| 50 to 70 | Low: treat with caution | 13% |
| Below 50 | Very low: often disordered regions | 5% |
What pLDDT means and how to read it
E1-like enzyme which specifically catalyzes the first step in ufmylation (PubMed:15071506, PubMed:18442052, PubMed:20368332, PubMed:25219498, PubMed:26929408, PubMed:27545674, PubMed:27545681, PubMed:27653677, PubMed:30412706, PubMed:30626644, PubMed:34588452). Activates UFM1 by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a UFM1-E1 thioester and free AMP (PubMed:20368332, PubMed:26929408, PubMed:27653677, PubMed:30412706). Activates UFM1 via a trans-binding mechanism, in which UFM1 interacts with distinct sites in both subunits of the UBA5 homodimer (PubMed:27653677). Trans-binding…
Homodimer; homodimerization is required for UFM1 activation (PubMed:27653677, PubMed:29295865). Interacts (via UIS motif) with UFM1; binds UFM1 via a trans-binding mechanism in which UFM1 interacts with distinct sites in both subunits of the UBA5 homodimer (PubMed:26872069, PubMed:26929408, PubMed:27653677, PubMed:28360427, PubMed:29295865, PubMed:30412706). Interacts (via C-terminus) with UFC1…
Cytoplasm, Nucleus, Endoplasmic reticulum membrane, Golgi apparatus
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 5IAA | X-ray | 1.85 Å | A/B=57-346 |
| 7NW1 | X-ray | 1.95 Å | CCC/FFF=389-404 |
| 3H8V | X-ray | 2.0 Å | A/B=57-329 |
| 5IA8 | X-ray | 2.0 Å | A/B=334-346 |
| 5L95 | X-ray | 2.1 Å | A/B=68-346 |
| 6H77 | X-ray | 2.1 Å | A/B/C/D=36-346 |
| 3GUC | X-ray | 2.25 Å | A/B=57-329 |
| 5HKH | X-ray | 2.55 Å | B=338-346 |
| 7NVK | X-ray | 2.65 Å | AAA=347-404 |
| 6H78 | X-ray | 2.7 Å | A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P=36-335 |
| 6H8C | NMR | B=333-348 | |
| 7OVC | NMR | B=381-404 |
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