Poly(U)-binding-splicing factor PUF60 (PUF60) is a 559-residue protein from Homo sapiens. This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: Q9UHX1.
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The mean pLDDT of this model is 67.9 (low overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 23% |
| 70 to 90 | Confident: backbone generally right | 34% |
| 50 to 70 | Low: treat with caution | 9% |
| Below 50 | Very low: often disordered regions | 34% |
What pLDDT means and how to read it
DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a…
Homodimer (PubMed:10606266). Associates with the spliceosome (PubMed:17579712). Found in a complex with RO60 and Y5 RNA (PubMed:10668799). Found in a complex with FUBP1 and far upstream element (FUSE) DNA segment (PubMed:10882074). Interacts directly with ERCC3 (PubMed:11239393). Interacts with CDK7 and GTF2H1 (PubMed:10882074). Interacts with SRSF11/P54 (PubMed:10606266). Does not interact with…
Nucleus
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 3UE2 | X-ray | 1.23 Å | A=443-559 |
| 3US5 | X-ray | 1.38 Å | A=443-559 |
| 7Z3X | X-ray | 1.65 Å | A/B=114-308 |
| 5KW6 | X-ray | 1.91 Å | A/B=118-316 |
| 6SLO | X-ray | 1.94 Å | A/B/C/D=460-559 |
| 5KVY | X-ray | 1.95 Å | A/B=118-316 |
| 6LUR | X-ray | 2.0 Å | A/B/C/D/E/F/G/H=460-559 |
| 7Q8A | X-ray | 2.05 Å | A/B=114-310 |
| 2QFJ | X-ray | 2.1 Å | A/B=118-316 |
| 5KW1 | X-ray | 2.1 Å | A/B=118-316 |
| 3DXB | X-ray | 2.2 Å | A/B/C/D/E/F/G/H=460-559 |
| 3UWT | X-ray | 2.5 Å | A=118-316 |
| 5KWQ | X-ray | 2.8 Å | A/B=118-316 |
| 2DNY | NMR | A=454-559 | |
| 2KXF | NMR | A=119-314 | |
| 2KXH | NMR | A=119-314 |
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