1A35: Human topoisomerase i/dna complex

Human topoisomerase i/dna complex. Determined by X-ray diffraction at 2.5 Å resolution. Released 28 Aug 1998.

Method
X-ray diffraction
Resolution
2.5 Å
Organism
Homo sapiens
Chains
3
Atoms
4,947
Mol. weight
84.04 kDa
Released
28 Aug 1998

Explore 1A35 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1A35 contains 29 α-helices and 23 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 29 helices, 23 β-strands

ElementResiduesLengthSheet
β-strand220-22231
β-strand22612
α-helix227-2315
α-helix233-2353
β-strand241-24223
β-strand245-24623
α-helix251-26111
α-helix267-2704
α-helix272-28312
α-helix290-2934
α-helix297-2993
β-strand300-30123
α-helix303-31715
α-helix321-33818
β-strand340-34341
β-strand346-34941
β-strand35014
β-strand35412
α-helix355-3573
β-strand359-36025
β-strand373-37425
α-helix375-3784
α-helix379-3813
β-strand383-38536
α-helix392-3943
β-strand403-40536
β-strand414-41746
β-strand424-42746
β-strand42914
α-helix434-46431
α-helix470-48516
α-helix491-4944
β-strand50817
α-helix509-5113
β-strand512-51878
β-strand521-530108
α-helix532-5343
β-strand536-54278
α-helix545-55511
β-strand56317
α-helix570-58011
α-helix586-60419
α-helix612-62918
β-strand63319
β-strand71419
α-helix717-7182
α-helix719-7235
α-helix726-73611
α-helix740-7434
α-helix746-7516
α-helix752-7543
α-helix755-7584

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
DNA (5'-d(*ap*ap*ap*ap*ap*gp*ap*cp*tp*tp*ap*gp*ap*ap*ap*ap*ap*(bru)p*(bru)p*tp*tp*t)-3')CDNA22
DNA (5'-d(*ap*ap*ap*ap*ap*tp*+up*+up*+up*+up*cp*+up*ap*ap*gp*tp*cp*tp*tp*tp*+ up*t)-3')DDNA22
Protein (DNA topoisomerase I)Aprotein591Homo sapiensP11387 (AlphaFold model)
Sequence of entity 1 (C), FASTA
>1A35_1 DNA (5'-D(*AP*AP*AP*AP*AP*GP*AP*CP*TP*TP*AP*GP*AP*AP*AP*AP*AP*(BRU)P*(BRU)P*TP*TP*T)-3') (chains C)
AAAAAGACTTAGAAAAAUUTTT
Sequence of entity 2 (D), FASTA
>1A35_2 DNA (5'-D(*AP*AP*AP*AP*AP*TP*+UP*+UP*+UP*+UP*CP*+UP*AP*AP*GP*TP*CP*TP*TP*TP*+ UP*T)-3') (chains D)
AAAAATUUUUCUAAGTCTTTUT
Sequence of entity 3 (A), FASTA
>1A35_3 PROTEIN (DNA TOPOISOMERASE I) (chains A)
KPKNKDKDKKVPEPDNKKKKPKKEEEQKWKWWEEERYPEGIKWKFLEHKGPVFAPPYEPL
PENVKFYYDGKVMKLSPKAEEVATFFAKMLDHEYTTKEIFRKNFFKDWRKEMTNEEKNII
TNLSKCDFTQMSQYFKAQTEARKQMSKEEKLKIKEENEKLLKEYGFCIMDNHKERIANFK
IEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDAKVPSPPPGHKWKEVRHDNKVTWLV
SWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCVDKIRNQYREDWKSKEMKVRQ
RAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHINLHPELDGQEYVVEFDFLGKDS
IRYYNKVPVEKRVFKNLQLFMENKQPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNA
SITLQQQLKELTAPDENIPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQTKIDAK
KEQLADARRDLKSAKADAKVMKDAKTKKVVESKKKAVQRLEEQLMKLEVQATDREENKQI
ALGTSKLNFLDPRITVAWCKKWGVPIEKIYNKTQREKFAWAIDMADEDYEF

Primary citation

Crystal structures of human topoisomerase I in covalent and noncovalent complexes with DNA. Redinbo, M.R., Stewart, L., Kuhn, P. et al. Science (1998) 279:1504-1513. DOI 10.1126/science.279.5356.1504 · PubMed

Other PDB entries of the same protein (UniProt P11387 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

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