1E8O: Core of the Alu domain of the mammalian SRP

Core of the Alu domain of the mammalian SRP. Determined by X-ray diffraction at 3.2 Å resolution. Released 8 Nov 2000.

Method: X-ray diffraction
Resolution: 3.2 Å
Organism: HOMO SAPIENS
Chains: 5
Atoms: 3,508
Mol. weight: 60.79 kDa
Released: 8 Nov 2000

Explore 1E8O in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1E8O contains 10 α-helices and 20 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 3 helices, 6 β-strands

Element	Residues	Length	Sheet
β-strand	3-4	2	1
α-helix	7-19	13
β-strand	26-31	6	1
β-strand	32	1	2
α-helix	33-35	3
β-strand	37	1	2
β-strand	38-43	6	1
β-strand	48-53	6	1
α-helix	57-72	16

Chain B: 2 helices, 3 β-strands

Element	Residues	Length	Sheet
α-helix	6-20	15
β-strand	26-33	8	1
β-strand	55-61	7	1
β-strand	66-72	7	1
α-helix	76-90	15

Chain C: 3 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	6-20	15
β-strand	26-29	4	3
β-strand	31	1	4
β-strand	32	1	5
β-strand	37	1	5
β-strand	38-43	6	3
β-strand	49-53	5	3
α-helix	56-58	3
α-helix	59-74	16

Chain D: 2 helices, 5 β-strands

Element	Residues	Length	Sheet
β-strand	3	1	3
α-helix	6-18	13
β-strand	26	1	4
β-strand	27-33	7	3
β-strand	55-61	7	3
β-strand	66-72	7	3
α-helix	76-90	15

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Signal recognition particle 9 kda protein	A, C	protein	85	HOMO SAPIENS	P49458 (AlphaFold model)
Signal recognition particle 14 kda protein	B, D	protein	106	HOMO SAPIENS	P37108 (AlphaFold model)
7SL RNA	E	RNA	50	HOMO SAPIENS

Sequence of entity 1 (A, C), FASTA

>1E8O_1 SIGNAL RECOGNITION PARTICLE 9 KDA PROTEIN (chains A, C)
PQYQTWEEFSRAAEKLYLADPMKARVVLKYRHSDGNLCVKVTDDLVCLVYKTDQAQDVKK
IEKFHSQLMRLMVAKEARNVTMETE

Sequence of entity 2 (B, D), FASTA

>1E8O_2 SIGNAL RECOGNITION PARTICLE 14 KDA PROTEIN (chains B, D)
VLLESEQFLTELTRLFQKCRTSGSVYITLKKYDGRTKPIPKKGTVEGFEPADNKCLLRAT
DGKKKISTVVSSKEVNKFQMAYSNLLRANMDGLKKRDKKNKTKKTK

Sequence of entity 3 (E), FASTA

>1E8O_3 7SL RNA (chains E)
GGGCCGGGCGCGGUGGCGCGCGCCUGUAGUCCCAGCUACUCGGGAGGCUC

Primary citation

Structure and Assembly of the Alu Domain of the Mammalian Signal Recognition Particle. Weichenrieder, O., Wild, K., Strub, K. et al. Nature (2000) 408:167. DOI 10.1038/35041507 · PubMed

Other PDB entries of the same protein (UniProt P49458 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5AOX 2.04 Å, Human Alu RNA retrotransposition complex in the ribosome-stalling conformation
7NFX 3.2 Å, Mammalian ribosome nascent chain complex with SRP and SRP receptor in early state A
4UYK 3.22 Å, Crystal structure of a Signal Recognition Particle Alu domain in the elongation arrest…
4UYJ 3.35 Å, Crystal structure of a Signal Recognition Particle Alu domain in the elongation arrest…
1E8S 4.0 Å, Alu domain of the mammalian SRP (potential Alu retroposition intermediate)
1RY1 12.0 Å, Structure of the signal recognition particle interacting with the elongation-arrested…

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