1JYE: Lactose Operon Repressor

Structure of a Dimeric Lac Repressor with C-terminal Deletion and K84L Substitution. Determined by X-ray diffraction at 1.7 Å resolution. Released 18 Oct 2001.

Method: X-ray diffraction
Resolution: 1.7 Å
Organism: Escherichia coli
Chains: 1
Atoms: 2,267
Mol. weight: 37.52 kDa
Released: 18 Oct 2001

Explore 1JYE in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1JYE contains 10 α-helices and 16 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 10 helices, 16 β-strands

Element	Residues	Length	Sheet
β-strand	63-68	6	1
α-helix	74-89	16
β-strand	93-98	6	1
α-helix	104-115	12
β-strand	122-125	4	1
α-helix	130-139	10
β-strand	145-147	3	1
β-strand	158-161	4	1
α-helix	163-177	15
β-strand	182-186	5	2
α-helix	192-207	16
β-strand	214-217	4	2
α-helix	222-234	13
β-strand	241-244	4	2
α-helix	247-259	13
β-strand	264	1	3
β-strand	268	1	3
β-strand	269-271	3	2
β-strand	274	1	4
α-helix	277-281	5
α-helix	285-286	2
β-strand	287	1	2
β-strand	288-290	3	4
α-helix	293-308	16
β-strand	316-319	4	1
β-strand	322-324	3	4

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Lactose Operon Repressor	A	protein	349	Escherichia coli	P03023 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>1JYE_1 Lactose Operon Repressor (chains A)
MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQ
SLLIGVATSSLALHAPSQIVAAILSRADQLGASVVVSMVERSGVEACKTAVHNLLAQRVS
GLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQ
QIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPT
AMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTS
VDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQL

Primary citation

Structure of a variant of lac repressor with increased thermostability and decreased affinity for operator. Bell, C.E., Barry, J., Matthews, K.S. et al. J Mol Biol (2001) 313:99-109. DOI 10.1006/jmbi.2001.5041 · PubMed

Other PDB entries of the same protein (UniProt P03023 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8GOH 1.1 Å, Homotetramic Antiparallel Coiled-Coil of 23-residues LacI C-terminal tetramerization helix
2P9H 2.0 Å, High resolution structure of the Lactose Repressor bound to IPTG
3EDC 2.1 Å, Crystal Structure of a 1.6-hexanediol Bound Tetrameric Form of Escherichia coli…
1EFA 2.6 Å, Crystal structure of the lac repressor dimer bound to operator and the anti-inducer onpf
1TLF 2.6 Å, Unprecedented quaternary structure of E. Coli lac repressor core tetramer: implications…
1LBI 2.7 Å, Lac repressor
4RZS 2.71 Å, Lac repressor engineered to bind sucralose, unliganded tetramer
1JYF 3.0 Å, Structure of the Dimeric Lac Repressor with an 11-residue C-terminal Deletion.
4RZT 3.1 Å, Lac repressor engineered to bind sucralose, sucralose-bound tetramer
1LBH 3.2 Å, Intact lactose operon repressor with gratuitous inducer iptg
2PAF 3.5 Å, Crystal Structure of the Lactose Repressor bound to anti-inducer ONPF in induced state
2PE5 3.5 Å, Crystal Structure of the Lac Repressor bound to ONPG in repressed state

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