1KF9: PDB entry 1KF9

Phage display derived variant of human growth hormone complexed with two copies of the extracellular domain of its receptor. Determined by X-ray diffraction at 2.6 Å resolution. Released 20 Nov 2002.

Method: X-ray diffraction
Resolution: 2.6 Å
Organism: Homo sapiens
Chains: 6
Atoms: 8,678
Mol. weight: 153.61 kDa
Released: 20 Nov 2002

Explore 1KF9 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1KF9 contains 33 α-helices and 70 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 0 β-strands

Element	Residues	Length
α-helix	6-35	30
α-helix	38-40	3
α-helix	43-46	4
α-helix	64-69	6
α-helix	74-85	12
α-helix	89-93	5
α-helix	94-98	5
α-helix	109-127	19
α-helix	157-181	25

Chain B: 4 helices, 18 β-strands

Element	Residues	Length	Sheet
β-strand	235-239	5	1
β-strand	240	1	2
β-strand	246-250	5	1
β-strand	265-270	6	3
β-strand	281-282	2	3
β-strand	293-296	4	1
β-strand	306-312	7	3
β-strand	317-324	8	3
α-helix	325-328	4
β-strand	329	1	2
α-helix	331-334	4
β-strand	335-344	10	4
β-strand	350-358	9	4
β-strand	372-380	9	5
α-helix	386	1
β-strand	387-388	2	5
α-helix	389-391	3
β-strand	392	1	5
β-strand	396-403	8	4
β-strand	407-416	10	5
β-strand	419	1	5
β-strand	429-432	4	5

Chain C: 3 helices, 17 β-strands

Element	Residues	Length	Sheet
β-strand	535-539	5	6
β-strand	540	1	7
β-strand	546-550	5	6
β-strand	567-570	4	8
β-strand	581-582	2	8
β-strand	593-596	4	6
β-strand	607-610	4	8
β-strand	622-623	2	8
β-strand	629	1	7
α-helix	631-634	4
β-strand	635-644	10	9
β-strand	650-658	9	9
β-strand	672-680	9	10
β-strand	687-688	2	10
α-helix	689-690	2
β-strand	692	1	10
β-strand	696-703	8	9
β-strand	707-716	10	10
α-helix	727-728	2
β-strand	729-732	4	10

Chain D: 9 helices, 0 β-strands

Element	Residues	Length
α-helix	1006-1035	30
α-helix	1038-1040	3
α-helix	1043-1045	3
α-helix	1064-1069	6
α-helix	1074-1085	12
α-helix	1089-1093	5
α-helix	1094-1098	5
α-helix	1109-1127	19
α-helix	1157-1181	25

Chain E: 4 helices, 18 β-strands

Element	Residues	Length	Sheet
β-strand	1235-1239	5	11
β-strand	1240	1	12
β-strand	1246-1250	5	11
β-strand	1265-1270	6	13
β-strand	1281-1282	2	13
β-strand	1293-1296	4	11
β-strand	1306-1312	7	13
β-strand	1317-1324	8	13
α-helix	1325-1328	4
β-strand	1329	1	12
α-helix	1331-1334	4
β-strand	1335-1344	10	14
β-strand	1350-1358	9	14
β-strand	1372-1380	9	15
α-helix	1386	1
β-strand	1387-1388	2	15
α-helix	1389-1391	3
β-strand	1392	1	15
β-strand	1396-1403	8	14
β-strand	1407-1416	10	15
β-strand	1419	1	15
β-strand	1429-1432	4	15

Chain F: 4 helices, 17 β-strands

Element	Residues	Length	Sheet
β-strand	1535-1539	5	16
β-strand	1540	1	17
β-strand	1546-1550	5	16
β-strand	1565-1570	6	18
β-strand	1581-1582	2	18
β-strand	1593-1596	4	16
β-strand	1607-1612	6	18
β-strand	1617-1623	7	18
β-strand	1629	1	17
α-helix	1631-1634	4
β-strand	1635-1644	10	19
β-strand	1650-1658	9	19
β-strand	1672-1680	9	20
α-helix	1686	1
β-strand	1687-1688	2	20
α-helix	1689-1690	2
β-strand	1692	1	20
β-strand	1696-1703	8	19
β-strand	1707-1716	10	20
α-helix	1727-1728	2
β-strand	1729-1732	4	20

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Phage display derived variant human growth hormone	A, D	protein	191	Homo sapiens	P01241 (AlphaFold model)
Extracellular domain human growth hormone receptor (1-238)	B, C, E, F	protein	238	Homo sapiens	P10912 (AlphaFold model)

Sequence of entity 1 (A, D), FASTA

>1KF9_1 PHAGE DISPLAY DERIVED VARIANT HUMAN GROWTH HORMONE (chains A, D)
FPTIPLSRLADNAWLRADRLNQLAFDTYQEFEEAYIPKEQIHSFWWNPQTSLCPSESIPT
PSNKEETQQKSNLELLRISLLLIQSWLEPVQFLRSVFANSLVYGASDSNVYDLLKDLEEG
IQTLMGRLEDGSPRTGQIFKQTYSKFDTNSHNDDALLKNYGLLYCFNKDMSKVSTYLRTV
QCRSVEGSCGF

Sequence of entity 2 (B, C, E, F), FASTA

>1KF9_2 EXTRACELLULAR DOMAIN HUMAN GROWTH HORMONE RECEPTOR (1-238) (chains B, C, E, F)
FSGSEATAAILSRAPWSLQSVNPGLKTNSSKEPKFTKCRSPERETFSCHWTDEVHHGTKN
LGPIQLFYTRRNTQEWTQEWKECPDYVSAGENSCYFNSSFTSIWIPYCIKLTSNGGTVDE
KCFSVDEIVQPDPPIALNWTLLNVSLTGIHADIQVRWEAPRNADIQKGWMVLEYELQYKE
VNETKWKMMDPILTTSVPVYSLKVDKEYEVRVRSKQRNSGNYGEFSEVLYVTLPQMSQ

Primary citation

Structure of a Phage Display Derived Variant of Human Growth Hormone Complexed to Two Copies of the Extracellular Domain of its Receptor: Evidence for Strong Structural Coupling between Receptor Binding Sites. Schiffer, C.A., Ultsch, M., Walsh, S. et al. J Mol Biol (2002) 316:277-289. DOI 10.1006/jmbi.2001.5348 · PubMed

Other PDB entries of the same protein (UniProt P01241 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1HUW 2.0 Å, The crystal structure of affinity-matured human growth hormone at 2 Å resolution
1AXI 2.1 Å, Structural plasticity at the hgh:hghbp interface
1HGU 2.5 Å, Human growth hormone
1HWG 2.5 Å, 1:2 complex of human growth hormone with its soluble binding protein
1A22 2.6 Å, Human growth hormone bound to single receptor
3HHR 2.8 Å, Human growth hormone and extracellular domain of its receptor: crystal structure of the…
1BP3 2.9 Å, The xray structure of a growth hormone-prolactin receptor complex
1HWH 2.9 Å, 1:1 complex of human growth hormone mutant G120R with its soluble binding protein

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