1MCT: Beta-trypsin

The refined 1.6 Å resolution crystal structure of the complex formed between porcine beta-trypsin and mcti-a, a trypsin inhibitor of squash family. Determined by X-ray diffraction at 1.6 Å resolution. Released 31 Jan 1994.

Method: X-ray diffraction
Resolution: 1.6 Å
Organisms: Sus scrofa, Momordica charantia
Chains: 2
Atoms: 1,991
Mol. weight: 26.7 kDa
Ligands: CA
Released: 31 Jan 1994

Explore 1MCT in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1MCT contains 9 α-helices and 24 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 8 helices, 20 β-strands

Element	Residues	Length	Sheet
β-strand	17	1	1
β-strand	20-21	2	2
α-helix	22-23	2
β-strand	30-34	5	3
β-strand	40-48	9	3
β-strand	51-54	4	3
α-helix	56-58	3
β-strand	64-67	4	3
β-strand	72	1	4
β-strand	81-90	10	3
β-strand	104-108	5	3
β-strand	122	1	2
α-helix	123-124	2
α-helix	128-130	3
β-strand	135-140	6	2
β-strand	154	1	4
β-strand	156-162	7	2
α-helix	163-164	2
α-helix	165-171	7
β-strand	180-183	4	2
β-strand	189	1	1
β-strand	198-201	4	2
β-strand	204-216	9	2
β-strand	221	1	5
β-strand	224	1	5
β-strand	226-230	5	2
α-helix	231-233	3
α-helix	235-244	10

Chain I: 1 helix, 4 β-strands

Element	Residues	Length	Sheet
β-strand	3-4	2	2
β-strand	9	1	6
α-helix	13-15	3
β-strand	22-23	2	6
β-strand	26-27	2	6

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Beta-trypsin	A	protein	223	Sus scrofa	P00761 (AlphaFold model)
Trypsin inhibitor a	I	protein	28	Momordica charantia	P30709 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>1MCT_1 BETA-TRYPSIN (chains A)
IVGGYTCAANSIPYQVSLNSGSHFCGGSLINSQWVVSAAHCYKSRIQVRLGEHNIDVLEG
NEQFINAAKIITHPNFNGNTLDNDIMLIKLSSPATLNSRVATVSLPRSCAAAGTECLISG
WGNTKSSGSSYPSLLQCLKAPVLSNSSCKSSYPGQITGNMICVGFLQGGKDSCQGDSGGP
VVCNGQLQGIVSWGYGCAQKNKPGVYTKVCNYVNWIQQTIAAN

Sequence of entity 2 (I), FASTA

>1MCT_2 TRYPSIN INHIBITOR A (chains I)
RICPRIWMECTRDSDCMAKCICVAGHCG

Ligands and cofactors

ID	Name	Formula	Copies
CA	Calcium ion	Ca	1

Primary citation

Refined 1.6 A resolution crystal structure of the complex formed between porcine beta-trypsin and MCTI-A, a trypsin inhibitor of the squash family. Detailed comparison with bovine beta-trypsin and its complex. Huang, Q., Liu, S., Tang, Y. J Mol Biol (1993) 229:1022-1036. DOI 10.1006/jmbi.1993.1102 · PubMed

Other PDB entries of the same protein (UniProt P00761 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9PDA 1.18 Å, Structure of Porcine Trypsin Crystals Grown From PEG and Complexed With Crystallization…
1S83 1.25 Å, Porcine trypsin complexed with 4-amino propanol
2A31 1.25 Å, Trypsin in complex with borate
9PDC 1.27 Å, Porcine Trypsin grown from PEG and Complexed with Crystallization Additives II
9PD9 1.28 Å, The Structure of Porcine Trypsin in Complex with Crystallization Additives I
1S5S 1.4 Å, Porcine trypsin complexed with guanidine-3-propanol inhibitor
9PCT 1.41 Å, Structure of Porcine Trypsin Crystals Grown from PEG Complexed with Crystallization…
1S6H 1.45 Å, Porcine trypsin complexed with guanidine-3-propanol inhibitor
1H9H 1.5 Å, Complex of eeti-II with porcine trypsin
2A32 1.5 Å, Trypsin in complex with benzene boronic acid
1FNI 1.6 Å, Crystal structure of porcine beta trypsin with 0.01% polydocanol
1QQU 1.63 Å, Crystal structure of porcine beta trypsin with bound acetate ion

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