1Q9E: RNase T1 variant with adenine specificity

RNase T1 variant with adenine specificity. Determined by X-ray diffraction at 1.7 Å resolution. Released 23 Mar 2004.

Method: X-ray diffraction
Resolution: 1.7 Å
Organism: Aspergillus oryzae
Chains: 3
Atoms: 2,688
Mol. weight: 33.63 kDa
Released: 23 Mar 2004

Explore 1Q9E in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1Q9E contains 15 α-helices and 31 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 6 helices, 11 β-strands

Element	Residues	Length	Sheet
β-strand	4-6	3	1
β-strand	9-11	3	1
α-helix	13-29	17
β-strand	33	1	2
β-strand	38	1	2
β-strand	40-42	3	3
α-helix	43	1
α-helix	53	1
α-helix	55	1
β-strand	56-60	5	3
β-strand	61	1	4
α-helix	66	1
β-strand	67	1	4
α-helix	68	1
β-strand	76-81	6	3
β-strand	86-91	6	3
β-strand	101-102	2	3

Chain B: 5 helices, 11 β-strands

Element	Residues	Length	Sheet
β-strand	4-6	3	5
β-strand	9-11	3	5
α-helix	13-28	16
β-strand	33	1	6
β-strand	38	1	6
β-strand	40-42	3	7
α-helix	53	1
α-helix	55	1
β-strand	56-60	5	7
β-strand	61	1	8
α-helix	66	1
β-strand	67	1	8
α-helix	68	1
β-strand	76-81	6	7
β-strand	86-91	6	7
β-strand	101-102	2	7

Chain C: 4 helices, 9 β-strands

Element	Residues	Length	Sheet
β-strand	4-6	3	9
β-strand	9-11	3	9
α-helix	13-29	17
β-strand	33	1	10
β-strand	38	1	10
β-strand	40-42	3	11
α-helix	53	1
α-helix	55	1
β-strand	56-60	5	11
α-helix	66-68	3
β-strand	76-81	6	11
β-strand	86-91	6	11
β-strand	101-102	2	11

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanyl-specific ribonuclease T1 precursor	A, B, C	protein	104	Aspergillus oryzae	P00651 (AlphaFold model)

Sequence of entity 1 (A, B, C), FASTA

>1Q9E_1 Guanyl-specific ribonuclease T1 precursor (chains A, B, C)
ACDYTCGSNCYSSSDVSTAQAAGYKLHEDGETVGSNSYPHEFRNWNGFDFSVSSPYYEWP
ILSSGDVYSGGSPGADRVVFNENNQLAGVITHTGASGNNFVECT

Primary citation

RNase T1 Variant RV Cleaves Single-Stranded RNA after Purines Due to Specific Recognition by the Asn46 Side Chain Amide. Czaja, R., Struhalla, M., Saenger, W. et al. Biochemistry (2004) 43:2854-2862. DOI 10.1021/bi035961f · PubMed

Other PDB entries of the same protein (UniProt P00651 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1I0V 1.23 Å, Ribonuclease T1 in complex with 2'GMP (form I crystal)
4ODK 1.4 Å, Structure of SlyD from Thermus thermophilus in complex with T1 peptide
9RNT 1.5 Å, Ribonuclease T1 with free recognition and catalytic site: crystal structure analysis at…
1LOV 1.55 Å, X-ray structure of the E58A mutant of Ribonuclease T1 complexed with 3'-guanosine…
1LOY 1.55 Å, X-ray structure of the H40A/E58A mutant of Ribonuclease T1 complexed with 3'-guanosine…
1I0X 1.65 Å, Ribonuclease T1 in complex with 2'GMP (form II crystal)
4GSP 1.65 Å, Ribonuclease T1 complexed with 2',3'-cgps + 3'-GMP, 7 days
1HYF 1.7 Å, Ribonuclease T1 V16A mutant in complex with SR2+
1RGA 1.7 Å, Crystal structure of RNase T1 with 3'-GMP and guanosine: a product complex
4BIR 1.7 Å, Ribonuclease T1: free HIS92GLN mutant
1I3I 1.76 Å, Ribonuclease T1 V78T mutant
3BU4 1.77 Å, Ribonuclease T1 complex with 2'GMP

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