1SK6: Adenylyl cyclase domain of anthrax edema factor

Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF) in complex with calmodulin, 3',5' cyclic AMP (cAMP), and pyrophosphate. Determined by X-ray diffraction at 3.2 Å resolution. Released 8 Jun 2004.

Method: X-ray diffraction
Resolution: 3.2 Å
Organisms: Bacillus anthracis, Homo sapiens
Chains: 6
Atoms: 15,050
Mol. weight: 229.91 kDa
Ligands: CA, POP, CMP, YB
Released: 8 Jun 2004

Explore 1SK6 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1SK6 contains 93 α-helices and 78 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 20 helices, 27 β-strands

Element	Residues	Length	Sheet
β-strand	296-297	2	1
α-helix	299-305	7
α-helix	309-321	13
β-strand	324-328	5	1
α-helix	333-335	3
α-helix	336-341	6
β-strand	344-345	2	2
β-strand	363	1	2
β-strand	365	1	3
α-helix	377-393	17
β-strand	395	1	3
β-strand	398-402	5	3
β-strand	404	1	4
α-helix	407-415	9
β-strand	420-421	2	5
β-strand	425-427	3	5
β-strand	430-436	7	5
β-strand	442-447	6	5
β-strand	452	1	4
β-strand	453-457	5	5
α-helix	458	1
β-strand	472-473	2	5
β-strand	475-481	7	3
β-strand	484-487	4	3
β-strand	488-489	2	2
β-strand	492	1	6
β-strand	494-499	6	1
β-strand	500	1	7
α-helix	501-505	5
α-helix	512-517	6
α-helix	524-533	10
α-helix	534-538	5
β-strand	541-542	2	8
β-strand	548-549	2	8
α-helix	554-565	12
β-strand	575	1	6
α-helix	580-582	3
β-strand	593-596	4	1
β-strand	602-605	4	1
α-helix	608-618	11
β-strand	624	1	7
α-helix	648-651	4
α-helix	660-668	9
α-helix	697-704	8
α-helix	714-731	18
α-helix	743-763	21
α-helix	788-796	9

Chain B: 19 helices, 20 β-strands

Element	Residues	Length	Sheet
β-strand	296-297	2	10
α-helix	299-301	3
α-helix	309-319	11
β-strand	324-329	6	10
α-helix	330-332	3
α-helix	333-340	8
β-strand	344-345	2	11
β-strand	365	1	12
α-helix	368-370	3
α-helix	377-393	17
β-strand	398-402	5	12
β-strand	404	1	13
α-helix	409-414	6
β-strand	420-426	7	14
β-strand	431-436	6	14
β-strand	442-447	6	14
β-strand	452	1	13
β-strand	453-457	5	14
β-strand	472-473	2	14
α-helix	474	1
β-strand	475-479	5	12
β-strand	486-487	2	12
β-strand	488-489	2	11
β-strand	493-499	7	10
α-helix	502-504	3
α-helix	512-516	5
α-helix	526-535	10
β-strand	541-542	2	15
β-strand	548-549	2	15
α-helix	551-565	15
α-helix	580-582	3
β-strand	594-596	3	10
β-strand	602-604	3	10
α-helix	608-615	8
α-helix	616-620	5
α-helix	648-650	3
α-helix	714-724	11
α-helix	745-761	17
α-helix	790-793	4

Chain C: 25 helices, 25 β-strands

Element	Residues	Length	Sheet
β-strand	297	1	17
α-helix	299-305	7
α-helix	309-321	13
β-strand	324-328	5	17
α-helix	333-340	8
α-helix	343	1
β-strand	344-345	2	18
α-helix	346	1
α-helix	352-355	4
β-strand	363	1	18
β-strand	365	1	19
α-helix	368-370	3
α-helix	377-392	16
β-strand	398-402	5	19
β-strand	404	1	20
α-helix	407-416	10
β-strand	420-427	8	21
β-strand	430-436	7	21
β-strand	442-447	6	21
β-strand	452	1	20
β-strand	453-457	5	21
β-strand	472-473	2	21
α-helix	474	1
β-strand	475-481	7	19
β-strand	484-487	4	19
β-strand	488-489	2	18
β-strand	492	1	22
β-strand	494-499	6	17
β-strand	500	1	23
α-helix	501-505	5
α-helix	512-517	6
α-helix	522-533	12
β-strand	541-542	2	24
β-strand	548-549	2	24
α-helix	552-567	16
β-strand	575	1	22
α-helix	580-582	3
β-strand	593-596	4	17
β-strand	602-605	4	17
α-helix	613-615	3
α-helix	616-620	5
β-strand	624	1	23
α-helix	650-652	3
α-helix	657-659	3
α-helix	666-669	4
α-helix	696-704	9
α-helix	707-709	3
α-helix	714-730	17
α-helix	743-764	22
α-helix	787-797	11

Chain D: 8 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	7-13	7
α-helix	33-38	6
α-helix	49-55	7
α-helix	68-78	11
α-helix	83-92	10
β-strand	99-100	2	9
α-helix	102-112	11
α-helix	120-128	9
β-strand	136-137	2	9
α-helix	138-144	7

Chain E: 10 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	7-10	4
α-helix	13-15	3
α-helix	29-31	3
α-helix	32-37	6
α-helix	45-55	11
α-helix	75-78	4
α-helix	83-92	10
β-strand	99-100	2	16
α-helix	102-111	10
α-helix	120-128	9
β-strand	136-137	2	16
α-helix	138-143	6

Chain F: 11 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	7-10	4
α-helix	11-17	7
α-helix	30-32	3
α-helix	33-36	4
α-helix	52-55	4
α-helix	65-75	11
α-helix	83-86	4
α-helix	89-92	4
β-strand	100	1	25
α-helix	102-110	9
α-helix	118-128	11
β-strand	136	1	25
α-helix	138-143	6

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Calmodulin-sensitive adenylate cyclase	A, B, C	protein	510	Bacillus anthracis	P40136 (AlphaFold model)
Calmodulin	D, E, F	protein	148	Homo sapiens	P0DP23 (AlphaFold model)

Sequence of entity 1 (A, B, C), FASTA

>1SK6_1 Calmodulin-sensitive adenylate cyclase (chains A, B, C)
DRIDVLKGEKALKASGLVPEHADAFKKIARELNTYILFRPVNKLATNLIKSGVATKGLNV
HGKSSDWGPVAGYIPFDQDLSKKHGQQLAVEKGNLENKKSITEHEGEIGKIPLKLDHLRI
EELKENGIILKGKKEIDNGKKYYLLESNNQVYEFRISDENNEVQYKTKEGKITVLGEKFN
WRNIEVMAKNVEGVLKPLTADYDLFALAPSLTEIKKQIPQKEWDKVVNTPNSLEKQKGVT
NLLIKYGIERKPDSTKGTLSNWQKQMLDRLNEAVKYTGYTGGDVVNHGTEQDNEEFPEKD
NEIFIINPEGEFILTKNWEMTGRFIEKNITGKDYLYYFNRSYNKIAPGNKAYIEWTDPIT
KAKINTIPTSAEFIKNLSSIRRSSNVGVYKDSGDKDEFAKKESVKKIAGYLSDYYNSANH
IFSQEKKRKISIFRGIQAYNEIENVLKSKQIAPEYKNYFQYLKERITNQVQLLLTHQKSN
IEFKLLYKQLNFTENETDNFEVFQKIIDEK

Sequence of entity 2 (D, E, F), FASTA

>1SK6_2 Calmodulin (chains D, E, F)
ADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGN
GTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEE
VDEMIREADIDGDGQVNYEEFVQMMTAK

Ligands and cofactors

ID	Name	Formula	Copies
CA	Calcium ion	Ca	6
POP	Pyrophosphate 2-	H2 O7 P2	3
CMP	Adenosine-3',5'-cyclic-monophosphate	C10 H12 N5 O6 P	3
YB	Ytterbium (III) ion	Yb	9

Primary citation

Structural and kinetic analyses of the interaction of anthrax adenylyl cyclase toxin with reaction products cAMP and pyrophosphate. Guo, Q., Shen, Y., Zhukovskaya, N.L. et al. J Biol Chem (2004) 279:29427-29435. DOI 10.1074/jbc.M402689200 · PubMed

Other PDB entries of the same protein (UniProt P40136 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1K8T 2.6 Å, Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF)
1K90 2.75 Å, Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF) in complex…
1K93 2.95 Å, Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF) in complex…
1S26 3.0 Å, Structure of Anthrax Edema Factor-Calmodulin-alpha,beta-methyleneadenosine…
1XFX 3.2 Å, Crystal structure of anthrax edema factor (EF) in complex with calmodulin in the…
6UZB 3.2 Å, Anthrax toxin protective antigen channels bound to edema factor
1XFZ 3.25 Å, Crystal structure of anthrax edema factor (EF) in complex with calmodulin in the…
1PK0 3.3 Å, Crystal Structure of the EF3-CaM complexed with PMEApp
1XFY 3.3 Å, Crystal structure of anthrax edema factor (EF) in complex with calmodulin
6VRA 3.3 Å, Anthrax octamer prechannel bound to full-length edema factor
1XFU 3.35 Å, Crystal structure of anthrax edema factor (EF) truncation mutant, EF-delta 64 in complex…
1XFV 3.35 Å, Crystal structure of anthrax edema factor (EF) in complex with calmodulin and 3' deoxy-ATP

Browse structure collections

About this viewer

MolViewer shows 1SK6 directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.