1TCS: Trichosanthin-NADPH complex

Crystal structure of trichosanthin-NADPH complex at 1.7 Å resolution reveals active-site architecture. Determined by X-ray diffraction at 1.7 Å resolution. Released 10 Jul 1995.

Method: X-ray diffraction
Resolution: 1.7 Å
Organism: Trichosanthes kirilowii
Chains: 1
Atoms: 2,107
Mol. weight: 27.91 kDa
Ligands: NDP
Released: 10 Jul 1995

Explore 1TCS in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1TCS contains 14 α-helices and 17 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 14 helices, 17 β-strands

Element	Residues	Length	Sheet
β-strand	2-5	4	1
α-helix	11-23	13
β-strand	27-31	5	2
β-strand	34-35	2	2
β-strand	36	1	3
β-strand	37	1	2
α-helix	38	1
α-helix	43-45	3
β-strand	47-53	7	1
β-strand	59-65	7	1
β-strand	71-76	6	1
β-strand	79-82	4	1
α-helix	86-94	9
β-strand	101-104	4	1
α-helix	111-118	8
α-helix	122-124	3
β-strand	127	1	4
α-helix	129-140	12
α-helix	147-155	9
α-helix	156-160	5
α-helix	161-163	3
β-strand	164	1	5
α-helix	165-172	8
β-strand	179	1	4
α-helix	180-182	3
α-helix	183-202	20
β-strand	208-216	9	6
β-strand	222-227	6	6
α-helix	231-235	5
β-strand	237	1	5
β-strand	240	1	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Trichosanthin	A	protein	247	Trichosanthes kirilowii	P09989 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>1TCS_1 TRICHOSANTHIN (chains A)
DVSFRLSGATSSSYGVFISNLRKALPNERKLYDIPLLRSSLPGSQRYALIHLTNYADETI
SVAIDVTNVYIMGYRAGDTSYFFNEASATEAAKYVFKDAMRKVTLPYSGNYERLQTAAGK
IRENIPLGLPALDSAITTLFYYNANSAASALMVLIQSTSEAARYKFIEQQIGKRVDKTFL
PSLAIISLENSWSALSKQIQIASTNNGQFESPVVLINAQNQRVTITNVDAGVVTSNIALL
LNRNNMA

Ligands and cofactors

ID	Name	Formula	Copies
NDP	NADPH dihydro-nicotinamide-adenine-dinucleotide phosphate	C21 H30 N7 O17 P3	1

Primary citation

Crystal structure of trichosanthin-NADPH complex at 1.7 A resolution reveals active-site architecture. Xiong, J.P., Xia, Z.X., Wang, Y. Nat Struct Biol (1994) 1:695-700. DOI 10.1038/nsb1094-695 · PubMed

Other PDB entries of the same protein (UniProt P09989 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1MRJ 1.6 Å, Studies on crystal structures active center geometry and depurine mechanism of two…
1MRK 1.6 Å, Studies on crystal structures active center geometry and depurine mechanism of two…
1GIS 1.7 Å, A trichosanthin(tcs) MUTANT(E85Q) complex structure with…
1GIU 1.8 Å, A trichosanthin(tcs) MUTANT(E85R) complex structure with adenine
1QD2 1.86 Å, Crystal structure of the complex of trichosanthin with adenine, obtained from…
1NLI 1.93 Å, Complex of [E160A-E189A] trichosanthin and adenine
1J4G 2.0 Å, crystal structure analysis of the trichosanthin delta C7
2JDL 2.2 Å, Structure of C-terminal region of acidic P2 ribosomal protein complexed with trichosanthin
2JJR 2.3 Å, V232K, N236D-trichosanthin
2VS6 2.4 Å, K173A, R174A, K177A-trichosanthin

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