1YSA: Protein

The GCN4 basic region leucine zipper binds DNA as a dimer of uninterrupted alpha helices: crystal structure of the protein-DNA complex. Determined by X-ray diffraction at 2.9 Å resolution. Released 31 Oct 1993.

Method: X-ray diffraction
Resolution: 2.9 Å
Organism: Saccharomyces cerevisiae
Chains: 4
Atoms: 1,772
Mol. weight: 26.05 kDa
Released: 31 Oct 1993

Explore 1YSA in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

1YSA contains 2 α-helices and 0 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain C: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	227-276	50

Chain D: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	228-277	50

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
DNA (5'-d(tptpcpcptpaptpgpapcptpcpaptpcpcpa pgptpt)-3')	A	DNA	20
DNA (5'-d(apapapcptpgpgpaptpgpapgptpcpaptpa pgpgpa)-3')	B	DNA	20
Protein (GCN4)	C, D	protein	58	Saccharomyces cerevisiae	P03069 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>1YSA_1 DNA (5'-D(*TP*TP*CP*CP*TP*AP*TP*GP*AP*CP*TP*CP*AP*TP*CP*CP*A P*GP*TP*T)-3') (chains A)
TTCCTATGACTCATCCAGTT

Sequence of entity 2 (B), FASTA

>1YSA_2 DNA (5'-D(*AP*AP*AP*CP*TP*GP*GP*AP*TP*GP*AP*GP*TP*CP*AP*TP*A P*GP*GP*A)-3') (chains B)
AAACTGGATGAGTCATAGGA

Sequence of entity 3 (C, D), FASTA

>1YSA_3 PROTEIN (GCN4) (chains C, D)
MKDPAALKRARNTEAARRSRARKLQRMKQLEDKVEELLSKNYHLENEVARLKKLVGER

Primary citation

The GCN4 basic region leucine zipper binds DNA as a dimer of uninterrupted alpha helices: crystal structure of the protein-DNA complex. Ellenberger, T.E., Brandl, C.J., Struhl, K. et al. Cell (1992) 71:1223-1237. DOI 10.1016/S0092-8674(05)80070-4 · PubMed

Other PDB entries of the same protein (UniProt P03069 (AlphaFold model), which also has an AlphaFold model), best resolution first:

3AZD 0.98 Å, Crystal structure of tropomyosin N-terminal fragment at 0.98A resolution
2WQ1 1.08 Å, GCN4 leucine zipper mutant with three IxxNTxx motifs coordinating bromide
2WQ0 1.12 Å, GCN4 leucine zipper mutant with three IxxNTxx motifs coordinating chloride
4OWI 1.2 Å, peptide structure
2WQ3 1.22 Å, GCN4 leucine zipper mutant with three IxxNTxx motifs coordinating chloride and nitrate
2HY6 1.25 Å, A seven-helix coiled coil
2WPZ 1.25 Å, GCN4 leucine zipper mutant with two VxxNxxx motifs coordinating chloride
6PSA 1.3 Å, PIE12 D-peptide against HIV entry (in complex with IQN17 Q577R resistance mutant)
2IPZ 1.35 Å, A Parallel Coiled-Coil Tetramer with Offset Helices
2YNY 1.35 Å, Salmonella enterica SadA 255-302 fused to GCN4 adaptors (SadAK1)
5APU 1.35 Å, Sequence IANKEDKAD inserted between GCN4 adaptors - Structure A9b black
2WQ2 1.36 Å, GCN4 leucine zipper mutant with three IxxNTxx motifs coordinating iodide

Browse more

1YSA is part of these collections:

DNA and RNA

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