23IW: Cannabinoid Receptor 1-Gi Complex
Cannabinoid Receptor 1-Gi Complex. Determined by electron microscopy at 2.42 Å resolution. Released 20 May 2026.
- Method
- Electron microscopy
- Resolution
- 2.42 Å
- Organisms
- Homo sapiens, Mus musculus
- Chains
- 5
- Atoms
- 8,543
- Mol. weight
- 169.17 kDa
- Ligands
- A1E5T
- Released
- 20 May 2026
Explore 23IW in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
23IW contains 33 α-helices and 59 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 10 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 7-31 | 25 | |
| β-strand | 33-40 | 8 | 1 |
| α-helix | 46-53 | 8 | |
| β-strand | 185-191 | 7 | 1 |
| β-strand | 194-200 | 7 | 1 |
| α-helix | 208-210 | 3 | |
| α-helix | 212-215 | 4 | |
| β-strand | 220-226 | 7 | 1 |
| α-helix | 227-229 | 3 | |
| α-helix | 242-254 | 13 | |
| β-strand | 263-269 | 7 | 1 |
| α-helix | 271-280 | 10 | |
| α-helix | 283-285 | 3 | |
| α-helix | 296-308 | 13 | |
| β-strand | 319-322 | 4 | 1 |
| α-helix | 331-350 | 20 | |
Chain B: 3 helices, 28 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 4-25 | 22 | |
| α-helix | 30-33 | 4 | |
| β-strand | 47-51 | 5 | 2 |
| β-strand | 58-63 | 6 | 3 |
| β-strand | 69-74 | 6 | 3 |
| β-strand | 78-83 | 6 | 3 |
| β-strand | 88-94 | 7 | 3 |
| β-strand | 100-105 | 6 | 4 |
| β-strand | 111-116 | 6 | 4 |
| β-strand | 120-125 | 6 | 4 |
| β-strand | 134-140 | 7 | 4 |
| β-strand | 146-151 | 6 | 5 |
| β-strand | 156-161 | 6 | 5 |
| β-strand | 165-170 | 6 | 5 |
| β-strand | 175-181 | 7 | 5 |
| β-strand | 187-192 | 6 | 6 |
| β-strand | 198-203 | 6 | 6 |
| β-strand | 207-212 | 6 | 6 |
| β-strand | 218-223 | 6 | 6 |
| β-strand | 229-234 | 6 | 7 |
| β-strand | 240-245 | 6 | 7 |
| β-strand | 250-254 | 5 | 7 |
| β-strand | 259-264 | 6 | 7 |
| α-helix | 272 | 1 | |
| β-strand | 273-278 | 6 | 8 |
| β-strand | 284-289 | 6 | 8 |
| β-strand | 294-298 | 5 | 8 |
| β-strand | 304-308 | 5 | 8 |
| β-strand | 315-320 | 6 | 2 |
| β-strand | 327-331 | 5 | 2 |
| β-strand | 336-339 | 4 | 2 |
Chain G: 4 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 7-23 | 17 | |
| α-helix | 30-43 | 14 | |
| α-helix | 53-55 | 3 | |
| α-helix | 56-58 | 3 | |
Chain R: 11 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 105-108 | 4 | |
| α-helix | 113-142 | 30 | |
| α-helix | 145-149 | 5 | |
| α-helix | 151-178 | 28 | |
| α-helix | 189-219 | 31 | |
| α-helix | 224-227 | 4 | |
| α-helix | 230-253 | 24 | |
| α-helix | 273-310 | 38 | |
| α-helix | 337-367 | 31 | |
| α-helix | 373-398 | 26 | |
| α-helix | 402-411 | 10 | |
Chain S: 5 helices, 25 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 3-7 | 5 | 9 |
| β-strand | 11-12 | 2 | 10 |
| β-strand | 18-25 | 8 | 9 |
| α-helix | 29-31 | 3 | |
| β-strand | 33-39 | 7 | 11 |
| β-strand | 45-51 | 7 | 11 |
| β-strand | 58-60 | 3 | 11 |
| β-strand | 68-73 | 6 | 9 |
| β-strand | 78-83 | 6 | 9 |
| α-helix | 88-90 | 3 | |
| β-strand | 92-99 | 8 | 11 |
| β-strand | 110-111 | 2 | 11 |
| β-strand | 115-117 | 3 | 11 |
| β-strand | 118-119 | 2 | 10 |
| α-helix | 125-127 | 3 | |
| β-strand | 128-129 | 2 | 12 |
| β-strand | 134-136 | 3 | 13 |
| β-strand | 143-149 | 7 | 12 |
| β-strand | 154 | 1 | 14 |
| β-strand | 160 | 1 | 14 |
| β-strand | 162-167 | 6 | 13 |
| β-strand | 174-178 | 5 | 13 |
| β-strand | 182-183 | 2 | 13 |
| α-helix | 184 | 1 | |
| β-strand | 191-195 | 5 | 12 |
| β-strand | 199-204 | 6 | 12 |
| β-strand | 214-219 | 6 | 13 |
| α-helix | 225 | 1 | |
| β-strand | 227 | 1 | 13 |
| β-strand | 231-234 | 4 | 13 |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Cannabinoid receptor 1 | R | protein | 504 | Homo sapiens | P21554 (AlphaFold model) |
| Guanine nucleotide-binding protein G(i) subunit alpha-1 | A | protein | 354 | Homo sapiens | P63096 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | B | protein | 339 | Homo sapiens | P62873 (AlphaFold model) |
| Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | G | protein | 71 | Homo sapiens | P59768 (AlphaFold model) |
| Scfv16 | S | protein | 250 | Mus musculus | |
Sequence of entity 1 (R), FASTA
>23IW_1 Cannabinoid receptor 1 (chains R)
MKTIIALSYIFCLVFADYKDDDDKLEVLFQGPMKSILDGLADTTFRTITTDLLYVGSNDI
QYEDIKGDMASKLGYFPQKFPLTSFRGSPFQEKMTAGDNPQLVPADQVNITEFYNKSLSS
FKENEENIQCGENFMDIECFMVLNPSQQLAIAVLSLTLGTFTVLENLLVLCVILHSRSLR
CRPSYHFIGSLAVADLLGSVIFVYSFIDFHVFHRKDSRNVFLFKLGGVTASFTASVGSLF
LTAIDRYISIHRPLAYKRIVTRPKAVVAFCLMWTIAIVIAVLPLLGWNCEKLQSVCSDIF
PHIDETYLMFWIGVTSVLLLFIVYAYMYILWKAHSHAVRMIQRGTQKSIIIHTSEDGKVQ
VTRPDQARMDIRLAKTLVLILVVLIICWGPLLAIMVYDVFGKMNKLIKTVFAFCSMLCLL
NSTVNPIIYALRSKDLRHAFRSMFPSCEGTAQPLDNSMGDSDCLHKHANNAASVHRAAES
CIKSTVKIAKVTMSVSTDTSAEAL
Sequence of entity 2 (A), FASTA
>23IW_2 Guanine nucleotide-binding protein G(i) subunit alpha-1 (chains A)
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKNTIVKQMKIIHEAG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TTGIVETHFTFKDLHFKMFDVGAQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM
NRMHASMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAA
AYIQCQFEDLNKRKDTKEIYTHFTCSTDTKNVQFVFDAVTDVIIKNNLKDCGLF
Sequence of entity 3 (B), FASTA
>23IW_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
SELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAM
HWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNIC
SIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFT
GHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAF
ATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALK
ADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN
Sequence of entity 4 (G), FASTA
>23IW_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL
Sequence of entity 5 (S), FASTA
>23IW_5 Scfv16 (chains S)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKGS
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| A1E5T | methyl (2~{S},3~{R})-2-[[1-[(4-fluorophenyl)methyl]indazol-3-yl]carbonylamino]-… | C20 H20 F N3 O4 | 1 |
Primary citation
Rational design of G i -biased CB1 agonist with reduced side effects. Liao, Y.Y., Che, J., Gao, Y.T. et al. Cell (2026) 189:3432. DOI 10.1016/j.cell.2026.03.020 · PubMed
Other PDB entries of the same protein (UniProt P21554 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 23IV 2.42 Å, Cannabinoid Receptor 1-Gi Complex
- 5U09 2.6 Å, High-resolution crystal structure of the human CB1 cannabinoid receptor
- 7FEE 2.7 Å, Crystal structure of the allosteric modulator ZCZ011 binding to CP55940-bound…
- 5TGZ 2.8 Å, Crystal Structure of the Human Cannabinoid Receptor CB1
- 5XRA 2.8 Å, Crystal structure of the human CB1 in complex with agonist AM11542
- 8GHV 2.8 Å, Cannabinoid Receptor 1-G Protein Complex
- 9B54 2.86 Å, Biased agonist bound CB1-Gi structure
- 8K8J 2.88 Å, Cannabinoid Receptor 1 bound to Fenofibrate coupling MiniGsq and Nb35 Complex
- 9ERX 2.9 Å, Structural basis of D9-THC analog activity at the Cannabinoid 1 receptor
- 5XR8 2.95 Å, Crystal structure of the human CB1 in complex with agonist AM841
- 6KPG 3.0 Å, Cryo-EM structure of CB1-G protein complex
- 6N4B 3.0 Å, Cannabinoid Receptor 1-G Protein Complex
Browse structure collections
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