27XK: A cryo-EM structure of GLP1-Y11-GLP1R-Gs complex

A cryo-EM structure of GLP1-Y11-GLP1R-Gs complex. Determined by electron microscopy at 2.64 Å resolution. Released 1 Jul 2026.

Method: Electron microscopy
Resolution: 2.64 Å
Organisms: Bos taurus, Rattus norvegicus, Homo sapiens
Chains: 6
Atoms: 9,440
Mol. weight: 159.53 kDa
Released: 1 Jul 2026

Explore 27XK in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

27XK contains 43 α-helices and 52 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 13 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	7-31	25
β-strand	33-40	8	1
α-helix	42-44	3
α-helix	46-53	8
β-strand	184-191	8	1
β-strand	194-201	8	1
α-helix	211-214	4
β-strand	220-226	7	1
α-helix	233-244	12
α-helix	247-249	3
β-strand	253-259	7	1
α-helix	261-269	9
α-helix	275-278	4
α-helix	280-283	4
α-helix	286-288	3
α-helix	293-294	2
α-helix	299-316	18
β-strand	328-330	3	1
α-helix	338-357	20

Chain B: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-25	22
α-helix	30-33	4
α-helix	38-40	3
β-strand	47-50	4	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	88-94	7	3
β-strand	100-105	6	4
β-strand	111-116	6	4
β-strand	121-125	5	4
β-strand	134-139	6	4
β-strand	146-151	6	5
β-strand	156-161	6	5
β-strand	165-170	6	5
β-strand	176-180	5	5
β-strand	187-192	6	6
β-strand	198-203	6	6
β-strand	208-212	5	6
β-strand	218-222	5	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	249-254	6	7
β-strand	259-264	6	7
β-strand	273-278	6	8
β-strand	284-289	6	8
β-strand	294-298	5	8
β-strand	304-308	5	8
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	336-339	4	2

Chain C: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	8-34	27

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	6-22	17
α-helix	30-43	14
α-helix	53-55	3

Chain N: 2 helices, 11 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	9
β-strand	10-11	2	10
β-strand	17-25	9	9
α-helix	29-31	3
β-strand	34-39	6	10
β-strand	45-51	7	10
β-strand	58-60	3	10
β-strand	68-73	6	9
β-strand	78-84	7	9
α-helix	88-90	3
β-strand	92-98	7	10
β-strand	118	1	10
β-strand	122-125	4	10

Chain R: 21 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	32-52	21
α-helix	54-56	3
α-helix	61	1
β-strand	62	1	11
α-helix	63-64	2
β-strand	66	1	12
β-strand	71	1	12
α-helix	72-74	3
β-strand	75	1	11
β-strand	79-84	6	13
α-helix	85-86	2
α-helix	92-94	3
β-strand	99-104	6	13
β-strand	110	1	13
α-helix	123-126	4
α-helix	138-168	31
α-helix	175-204	30
α-helix	224-256	33
α-helix	263-270	8
α-helix	271-275	5
α-helix	276-279	4
α-helix	281-291	11
α-helix	307-337	31
α-helix	346-360	15
α-helix	362-366	5
α-helix	382-399	18
α-helix	400-404	5
α-helix	407-421	15

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(s) subunit alpha	A	protein	361	Bos taurus	A0A5A9NRD5 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	371	Rattus norvegicus	P54311 (AlphaFold model)
Glucagon-like peptide 1(7-36)	C	protein	31	Homo sapiens	P01275 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	71	Bos taurus	P63212 (AlphaFold model)
Nanobody-35	N	protein	140	Lama glama
Glucagon-like peptide 1 receptor	R	protein	440	Homo sapiens	P43220

Sequence of entity 1 (A), FASTA

>27XK_1 Guanine nucleotide-binding protein G(s) subunit alpha (chains A)
MGCTLSAEDKAAVERSKMIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQMRIYHVNG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNDF
KSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPR
VTRAKYFIRDEFLRISTASGDGRHYCYPHFTCSVDTENARRIFNDCRDIIQRMHLRQYEL
L

Sequence of entity 2 (B), FASTA

>27XK_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHL
AKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACG
GLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQ
QTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFF
PNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCN
VWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWNGSSGGGGSGGGGSSG
VSGWRLFKKIS

Sequence of entity 3 (C), FASTA

>27XK_3 Glucagon-like peptide 1(7-36) (chains C)
HAEGYFTSDVSSYLEGQAAKEFIAWLVKGRX

Sequence of entity 4 (G), FASTA

>27XK_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 5 (N), FASTA

>27XK_5 Nanobody-35 (chains N)
MAQVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVRQAPGKGLEWVSDISQSGASI
SYTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCARCPAPFTRDCFDVTSTTYAYR
GQGTQVTVSSHHHHHHEPEA

Sequence of entity 6 (R), FASTA

>27XK_6 Glucagon-like peptide 1 receptor (chains R)
RPQGATVSLWETVQKWREYRRQCQRSLTEDPPPATDLFCNRTFDEYACWPDGEPGSFVNV
SCPWYLPWASSVPQGHVYRFCTAEGLWLQKDNSSLPWRDLSECEESKRGERSSPEEQLLF
LYIIYTVGYALSFSALVIASAILLGFRHLHCTRNYIHLNLFASFILRALSVFIKDAALKW
MYSTAAQQHQWDGLLSYQDSLSCRLVFLLMQYCVAANYYWLLVEGVYLYTLLAFSVLSEQ
WIFRLYVSIGWGVPLLFVVPWGIVKYLYEDEGCWTRNSNMNYWLIIRLPILFAIGVNFLI
FVRVICIVVSKLKANLMCKTDIKCRLAKSTLTLIPLLGTHEVIFAFVMDEHARGTLRFIK
LFTELSFTSFQGLMVAILYCFVNNEVQLEFRKSWERWRLEHLHIQRDSSMKPLKCPTSSL
SSGATAGSSMYTATCQASCS

Primary citation

N-terminally modified GLP1R agonists drive G protein bias via extracellular loop 3 displacement. Zhao, L., Yuan, Q. To be published.

Other PDB entries of the same protein (UniProt A0A5A9NRD5 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9JCO 2.36 Å, Cryo-EM structure of the proton-sensing GPCR (GPR4)-Gs protein complex at pH 6.5
9X3Z 2.54 Å, NPFF bound Mas1 Receptor Complex
9UWM 3.1 Å, A combined cryo_EM structure of Cagrilintide-CTR-Gs complex
9UWQ 3.1 Å, A combined Cryo_EM structure of Cagrilintide-AMY1R-Gs complex

Browse structure collections

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