2GH7: Epi-biotin complex with core streptavidin

Epi-biotin complex with core streptavidin. Determined by X-ray diffraction at 1.0 Å resolution. Released 18 Apr 2006.

Method: X-ray diffraction
Resolution: 1.0 Å
Organism: Streptomyces avidinii
Chains: 2
Atoms: 2,209
Mol. weight: 27.82 kDa
Ligands: BTQ, BTN
Released: 18 Apr 2006

Explore 2GH7 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2GH7 contains 4 α-helices and 16 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 3 helices, 8 β-strands

Element	Residues	Length	Sheet
β-strand	19-23	5	1
β-strand	28-33	6	1
β-strand	38-44	7	1
β-strand	54-60	7	1
α-helix	69-70	2
β-strand	71-80	10	1
β-strand	85-97	13	1
β-strand	103-112	10	1
α-helix	113	1
α-helix	116-121	6
β-strand	123-133	11	1

Chain B: 1 helix, 8 β-strands

Element	Residues	Length	Sheet
β-strand	19-23	5	2
β-strand	28-33	6	2
β-strand	38-44	7	2
β-strand	54-60	7	2
β-strand	71-80	10	2
β-strand	85-97	13	2
β-strand	103-112	10	2
α-helix	116-121	6
β-strand	123-131	9	2

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Streptavidin	A, B	protein	127	Streptomyces avidinii	P22629 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>2GH7_1 Streptavidin (chains A, B)
AEAGITGTWYNQLGSTFIVTAGADGALTGTYESAVGNAESRYVLTGRYDSAPATDGSGTA
LGWTVAWKNNYRNAHSATTWSGQYVGGAEARINTQWLLTSGTTEANAWKSTLVGHDTFTK
VKPSAAS

Ligands and cofactors

ID	Name	Formula	Copies
BTQ	Epi-biotin	C10 H16 N2 O3 S	2
BTN	Biotin	C10 H16 N2 O3 S	2

Water and common crystallization additives (GOL) are not listed.

Primary citation

The high-resolution structure of (+)-epi-biotin bound to streptavidin. Le Trong, I., Aubert, D.G., Thomas, N.R. et al. Acta Crystallogr D Biol Crystallogr (2006) 62:576-581. DOI 10.1107/S0907444906011887 · PubMed

Other PDB entries of the same protein (UniProt P22629 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2F01 0.85 Å, Epi-biotin complex with core streptavidin
9PUA 0.94 Å, L-Biotin-streptavidin binding
3RY2 0.95 Å, Wild-type core streptavidin-biotin complex at atomic resolution
9PUB 0.95 Å, Biotin-streptavidin binding
1LUQ 0.96 Å, Full Matrix Error Analysis of Streptavidin
3WYQ 1.0 Å, Crystal structure of the low-immunogenic core streptavidin mutant LISA-314…
4GDA 1.0 Å, Circular Permuted Streptavidin A50/N49
6T2L 1.0 Å, Streptavidin variants harbouring an artificial organocatalyst based cofactor
3RY1 1.03 Å, Wild-type core streptavidin at atomic resolution
5N8B 1.03 Å, Crystal structure of streptavidin with peptide afpdylaeyhgg
5N8J 1.05 Å, CRYSTAL STRUCTURE OF STREPTAVIDIN WITH PEPTIDE D-amino acid containing peptide…
4CPE 1.06 Å, Wild-type streptavidin in complex with love-hate ligand 1 (LH1)

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