2IBM: Preprotein translocase secA subunit

A novel dimer interface and conformational changes revealed by an X-ray structure of B. subtilis SecA. Determined by X-ray diffraction at 3.2 Å resolution. Released 14 Nov 2006.

Method

X-ray diffraction

Resolution

3.2 Å

Organism

Bacillus subtilis

Chains

2

Atoms

12,403

Mol. weight

178.26 kDa

Ligands

ADP

Released

14 Nov 2006

Explore 2IBM in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2IBM contains 83 α-helices and 43 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 42 helices, 22 β-strands

Molecules and chains

>2IBM_1 Preprotein translocase secA subunit (chains A, B)
MLGILNKMFDPTKRTLNRYEKIANDIDAIRGDYENLSDDALKHKTIEFKERLEKGATTDD
LLVEAFAVVREASRRVTGMFPFKVQLMGGVALHDGNIAEMKTGEGKTLTSTLPVYLNALT
GKGVHVVTVNEYLASRDAEQMGKIFEFLGLTVGLNLNSMSKDEKREAYAADITYSTNNEL
GFDYLRDNMVLYKEQMVQRPLHFAVIDEVDSILIDEARTPLIISGQAAKSTKLYVQANAF
VRTLKAEKDYTYDIKTKAVQLTEEGMTKAEKAFGIDNLFDVKHVALNHHINQALKAHVAM
QKDVDYVVEDGQVVIVDSFTGRLMKGRRYSEGLHQAIEAKEGLEIQNESMTLATITFQNY
FRMYEKLAGMTGTAKTEEEEFRNIYNMQVVTIPTNRPVVRDDRPDLIYRTMEGKFKAVAE
DVAQRYMTGQPVLVGTVAVETSELISKLLKNKGIPHQVLNAKNHEREAQIIEEAGQKGAV
TIATNMAGRGTDIKLGEGVKELGGLAVVGTERHESRRIDNQLRGRSGRQGDPGITQFYLS
MEDELMRRFGAERTMAMLDRFGMDDSTPIQSKMVSRAVESSQKRVEGNNFDSRKQLLQYD
DVLRQQREVIYKQRFEVIDSENLREIVENMIKSSLERAIAAYTPREELPEEWKLDGLVDL
INTTYLDEGALEKSDIFGKEPDEMLELIMDRIITKYNEKEEQFGKEQMREFEKVIVLRAV
DSKWMDHIDAMDQLRQGIHLRAYAQTNPLREYQMEGFAMFEHMIESIEDEVAKFVMKAEI

Ligands and cofactors

Primary citation

A Novel Dimer Interface and Conformational Changes Revealed by an X-ray Structure of B. subtilis SecA. Zimmer, J., Li, W., Rapoport, T.A. J Mol Biol (2006) 364:259-265. DOI 10.1016/j.jmb.2006.08.044 · PubMed

Related structures

Other PDB entries of the same protein (UniProt P28366 (AlphaFold model), which also has an AlphaFold model), best resolution first:

• 1TF5 2.18 Å, Crystal structure of SecA in an open conformation from Bacillus Subtilis
• 3JV2 2.5 Å, Crystal Structure of B. subtilis SecA with bound peptide
• 1M6N 2.7 Å, Crystal structure of the SecA translocation ATPase from Bacillus subtilis
• 1TF2 2.9 Å, Crystal structure of SecA:ADP in an open conformation from Bacillus Subtilis
• 8YA0 2.97 Å, Structure of the SecA-SecY complex with the substrate FtsQ-LacY(+7C)
• 1M74 3.0 Å, Crystal structure of Mg-ADP-bound SecA from Bacillus subtilis
• 8Y9Y 3.29 Å, Structure of the SecA-SecY complex with the substrate FtsQ-LacY(+1C)
• 3IQY 3.3 Å, Active site mutants of B. subtilis SecA
• 7XHB 3.33 Å, Structure of the SecA/SecYE/proOmpA(4Y)-sfGFP complex with ADP
• 7XHA 3.35 Å, Structure of the SecA/SecYE/proOmpA(4Y)-sfGFP complex with ADP.BeF3-.
• 3IQM 3.4 Å, Active site mutants of B. subtilis SecA
• 8Y9Z 3.41 Å, Structure of the SecA-SecY complex with the substrate HmBRI-3TM

Browse structure collections

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