2IHB: PDB entry 2IHB

Crystal structure of the heterodimeric complex of human RGS10 and activated Gi alpha 3. Determined by X-ray diffraction at 2.71 Å resolution. Released 21 Nov 2006.

Method: X-ray diffraction
Resolution: 2.71 Å
Organism: Homo sapiens
Chains: 2
Atoms: 3,451
Mol. weight: 55.79 kDa
Ligands: ALF, MG, GDP
Released: 21 Nov 2006

Explore 2IHB in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2IHB contains 30 α-helices and 8 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 21 helices, 8 β-strands

Element	Residues	Length	Sheet
β-strand	34-39	6	1
α-helix	46-57	12
α-helix	63-67	5
α-helix	70-91	22
α-helix	93-95	3
α-helix	100-109	10
α-helix	111-114	4
α-helix	121-132	12
α-helix	134-141	8
α-helix	143-145	3
α-helix	152-156	5
α-helix	159-163	5
α-helix	171-175	5
β-strand	184-191	8	1
β-strand	194-201	8	1
α-helix	205-208	4
α-helix	211-214	4
β-strand	220-226	7	1
α-helix	227-231	5
β-strand	233	1	2
β-strand	241	1	2
α-helix	242-254	13
α-helix	257-259	3
β-strand	263-269	7	1
α-helix	271-280	10
α-helix	283-285	3
α-helix	296-308	13
β-strand	319-323	5	1
α-helix	329-345	17

Chain B: 9 helices, 0 β-strands

Element	Residues	Length
α-helix	25-32	8
α-helix	34-39	6
α-helix	41-53	13
α-helix	57-71	15
α-helix	75-85	11
α-helix	86-90	5
α-helix	120-129	10
α-helix	130-134	5
α-helix	135-138	4

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(k) subunit alpha	A	protein	323	Homo sapiens	P08754 (AlphaFold model)
Regulator of G-protein signalling 10	B	protein	153	Homo sapiens	O43665 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>2IHB_1 Guanine nucleotide-binding protein G(k) subunit alpha (chains A)
KEVKLLLLGAGESGKSTIVKQMKIIHEDGYSEDECKQYKVVVYSNTIQSIIAIIRAMGRL
KIDFGEAARADDARQLFVLAGSAEEGVMTPELAGVIKRLWRDGGVQACFSRSREYQLNDS
ASYYLNDLDRISQSNYIPTQQDVLRTRVKTTGIVETHFTFKDLYFKMFDVGGQRSERKKW
IHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTETSIILFLNKK
DLFEEKIKRSPLTICYPEYTGSNTYEEAAAYIQCQFEDLNRRKDTKEIYTHFTCATDTKN
VQFVFDAVTDVIIKNNLKECGLY

Sequence of entity 2 (B), FASTA

>2IHB_2 Regulator of G-protein signalling 10 (chains B)
SMSRKRPPSDIHDSDGSSSSSHQSLKSTAKWAASLENLLEDPEGVKRFREFLKKEFSEEN
VLFWLACEDFKKMQDKTQMQEKAKEIYMTFLSSKASSQVNVEGQSRLNEKILEEPHPLMF
QKLQDQIFNLMKYDSYSRFLKSDLFLKHKRTEE

Ligands and cofactors

ID	Name	Formula	Copies
ALF	Tetrafluoroaluminate ion	Al F4	1
MG	Magnesium ion	Mg	1
GDP	Guanosine-5'-diphosphate	C10 H15 N5 O11 P2	1

Primary citation

Structural diversity in the RGS domain and its interaction with heterotrimeric G protein alpha-subunits. Soundararajan, M., Willard, F.S., Kimple, A.J. et al. Proc Natl Acad Sci U S A (2008) 105:6457-6462. DOI 10.1073/pnas.0801508105 · PubMed

Other PDB entries of the same protein (UniProt P08754 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2ODE 1.9 Å, Crystal structure of the heterodimeric complex of human RGS8 and activated Gi alpha 3
9YCX 2.1 Å, Neurotensin Receptor 1 (NTSR1) bound to Octotensin in complex with Gi3 in the…
9YCY 2.2 Å, Neurotensin Receptor 1 (NTSR1) bound to Octotensin in complex with Gi3 in the Canonical…
8IKL 2.33 Å, Cryo-EM structure of the CD97-G13 complex
8YK0 2.4 Å, Cryo-EM structure of human GPR156-Gi3 complex
7T10 2.5 Å, CryoEM structure of somatostatin receptor 2 in complex with somatostatin-14 and Gi3
9VJ6 2.62 Å, Cryo-EM structure of 5-HT1AR-Gi3 in complex with F-15599
7T11 2.7 Å, CryoEM structure of somatostatin receptor 2 in complex with Octreotide and Gi3.
9VJF 2.7 Å, Cryo-EM structure of 5-HT1AR-Gi3 in complex with buspirone
2V4Z 2.8 Å, The crystal structure of the human G-protein subunit alpha (GNAI3) in complex with an…
7KH0 2.8 Å, Cryo-EM structure of the human arginine vasopressin AVP-vasopressin receptor V2R-Gs…
4G5O 2.9 Å, Structure of LGN GL4/Galphai3(Q147L) complex

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