2KY6: ARC92VBD/MED25ACID

Structure of ARC92VBD/MED25ACID. Determined by solution NMR. Released 9 Mar 2011.

Method: Solution NMR
Organism: Homo sapiens
Chains: 1
Atoms: 1,334
Mol. weight: 18.98 kDa
Released: 9 Mar 2011

Explore 2KY6 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2KY6 contains 6 α-helices and 7 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 6 helices, 7 β-strands

Element	Residues	Length	Sheet
β-strand	398-409	12	1
α-helix	410-411	2
β-strand	424-434	11	1
α-helix	441-443	3
β-strand	447-454	8	1
α-helix	455-461	7
α-helix	462-465	4
β-strand	468-475	8	1
α-helix	480-493	14
β-strand	494-499	6	1
β-strand	510-515	6	1
β-strand	522-527	6	1
α-helix	530-545	16

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Mediator of RNA polymerase II transcription subunit 25	A	protein	166	Homo sapiens	Q71SY5 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>2KY6_1 Mediator of RNA polymerase II transcription subunit 25 (chains A)
GEFGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQK
LIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCE
VRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLE

Primary citation

Structure of the VP16 transactivator target in the Mediator. Milbradt, A.G., Kulkarni, M., Yi, T. et al. Nat Struct Mol Biol (2011) 18:410-415. DOI 10.1038/nsmb.1999 · PubMed

Other PDB entries of the same protein (UniProt Q71SY5 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9CCV 2.53 Å, Crystal structure of human respiratory syncytial virus NS1 bound to human MED25 ACID
7EMF 3.5 Å, Human Mediator (deletion of MED1-IDR) in a Tail-extended conformation
8GXS 4.16 Å, PIC-Mediator in complex with +1 nucleosome (T40N) in H-binding state
8T9D 4.66 Å, CryoEM structure of TR-TRAP
7LBM 4.8 Å, Structure of the human Mediator-bound transcription pre-initiation complex
8GXQ 5.04 Å, PIC-Mediator in complex with +1 nucleosome (T40N) in MH-binding state
2L23 NMR structure of the ACID (ACtivator Interacting Domain) of the human mediator Med25…
2L6U Solution NMR Structure of Med25(391-543) Comprising the Activator-Interacting Domain…
2XNF The Mediator Med25 activator interaction domain: Structure and cooperative binding of…

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