8GXQ: PIC-Mediator
PIC-Mediator in complex with +1 nucleosome (T40N) in MH-binding state. Determined by electron microscopy at 5.04 Å resolution. Released 2 Nov 2022.
- Method
- Electron microscopy
- Resolution
- 5.04 Å
- Organisms
- synthetic construct, Homo sapiens, Xenopus laevis
- Chains
- 87
- Atoms
- 185,972
- Mol. weight
- 4274.92 kDa
- Ligands
- MG, SF4, ZN
- Released
- 2 Nov 2022
Explore 8GXQ in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
8GXQ contains 1,048 α-helices and 794 β-strands across 83 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain a: 15 helices, 22 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 57-69 | 13 | |
| α-helix | 74-87 | 14 | |
| β-strand | 91-95 | 5 | 61 |
| β-strand | 99-104 | 6 | 61 |
| β-strand | 107-111 | 5 | 61 |
| α-helix | 136-143 | 8 | |
| α-helix | 147-160 | 14 | |
| α-helix | 168-194 | 27 | |
| α-helix | 199-205 | 7 | |
| β-strand | 210-212 | 3 | 62 |
| β-strand | 215 | 1 | 63 |
| β-strand | 218 | 1 | 63 |
| α-helix | 219 | 1 | |
| β-strand | 220-225 | 6 | 62 |
| α-helix | 228-231 | 4 | |
| β-strand | 253-261 | 9 | 62 |
| β-strand | 268-269 | 2 | 64 |
| α-helix | 285-287 | 3 | |
| β-strand | 296-297 | 2 | 64 |
| β-strand | 301-306 | 6 | 62 |
| α-helix | 310 | 1 | |
| β-strand | 311-314 | 4 | 65 |
| α-helix | 315-324 | 10 | |
| β-strand | 337-338 | 2 | 65 |
| α-helix | 339-348 | 10 | |
| β-strand | 362-366 | 5 | 66 |
| β-strand | 371-375 | 5 | 66 |
| β-strand | 389-393 | 5 | 65 |
| β-strand | 397-398 | 2 | 62 |
| α-helix | 404-422 | 19 | |
| β-strand | 440-446 | 7 | 66 |
| β-strand | 449-455 | 7 | 66 |
| β-strand | 462-466 | 5 | 66 |
| β-strand | 468 | 1 | 67 |
| β-strand | 476 | 1 | 67 |
| α-helix | 491-500 | 10 | |
| α-helix | 505-517 | 13 | |
Chain b: 3 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 56-89 | 34 | |
| α-helix | 102-135 | 34 | |
| α-helix | 157-185 | 29 | |
Chain BA: 16 helices, 4 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 23 | 1 | |
| β-strand | 24-25 | 2 | 12 |
| β-strand | 32-33 | 2 | 12 |
| β-strand | 39 | 1 | 13 |
| β-strand | 40-41 | 2 | 12 |
| α-helix | 48-51 | 4 | |
| α-helix | 109-127 | 19 | |
| α-helix | 132-148 | 17 | |
| α-helix | 156-171 | 16 | |
| α-helix | 177-183 | 7 | |
| α-helix | 188-202 | 15 | |
| α-helix | 207-209 | 3 | |
| α-helix | 211-222 | 12 | |
| α-helix | 226-242 | 17 | |
| α-helix | 250-264 | 15 | |
| α-helix | 271-278 | 8 | |
| α-helix | 282-292 | 11 | |
| α-helix | 293-295 | 3 | |
| α-helix | 296-299 | 4 | |
| α-helix | 310-312 | 3 | |
Chain c: 7 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 8-37 | 30 | |
| α-helix | 45-76 | 32 | |
| α-helix | 105-130 | 26 | |
| α-helix | 159-171 | 13 | |
| β-strand | 176-181 | 6 | 170 |
| β-strand | 189-194 | 6 | 170 |
| β-strand | 198-206 | 9 | 170 |
| β-strand | 208-216 | 9 | 170 |
| α-helix | 237-252 | 16 | |
| α-helix | 261-271 | 11 | |
| β-strand | 296-298 | 3 | 171 |
| β-strand | 301-305 | 5 | 171 |
| α-helix | 307-309 | 3 | |
Chain d: 5 helices, 1 β-strand
| Element | Residues | Length | Sheet |
|---|
| α-helix | 27-51 | 25 | |
| α-helix | 63-141 | 79 | |
| β-strand | 143 | 1 | 70 |
| α-helix | 145-156 | 12 | |
| α-helix | 180-184 | 5 | |
| α-helix | 186-191 | 6 | |
Chain DA: 30 helices, 17 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 347-353 | 7 | |
| α-helix | 399-402 | 4 | |
| α-helix | 404-407 | 4 | |
| α-helix | 410-413 | 4 | |
| α-helix | 471-472 | 2 | |
| α-helix | 473-477 | 5 | |
| α-helix | 480-483 | 4 | |
| α-helix | 570-573 | 4 | |
| α-helix | 593-596 | 4 | |
| α-helix | 609-613 | 5 | |
| α-helix | 621-623 | 3 | |
| β-strand | 634-638 | 5 | 19 |
| α-helix | 641-656 | 16 | |
| α-helix | 668-671 | 4 | |
| β-strand | 679-683 | 5 | 19 |
| α-helix | 692-693 | 2 | |
| β-strand | 697-704 | 8 | 19 |
| α-helix | 705-707 | 3 | |
| α-helix | 714-716 | 3 | |
| β-strand | 721-723 | 3 | 19 |
| α-helix | 734-735 | 2 | |
| β-strand | 739-744 | 6 | 19 |
| β-strand | 748-756 | 9 | 19 |
| α-helix | 757-758 | 2 | |
| β-strand | 761-766 | 6 | 19 |
| β-strand | 771-775 | 5 | 19 |
| β-strand | 778-782 | 5 | 19 |
| β-strand | 786-787 | 2 | 19 |
| α-helix | 790-793 | 4 | |
| α-helix | 797-816 | 20 | |
| β-strand | 824-825 | 2 | 20 |
| α-helix | 827-833 | 7 | |
| α-helix | 839-847 | 9 | |
| β-strand | 850-853 | 4 | 20 |
| β-strand | 861-864 | 4 | 20 |
| α-helix | 865 | 1 | |
| α-helix | 873-879 | 7 | |
| α-helix | 882-900 | 19 | |
| α-helix | 925-928 | 4 | |
| α-helix | 931-943 | 13 | |
| β-strand | 947-948 | 2 | 21 |
| β-strand | 967-968 | 2 | 21 |
| α-helix | 1056-1078 | 23 | |
| α-helix | 1154-1157 | 4 | |
| β-strand | 1165-1170 | 6 | 22 |
| β-strand | 1180-1185 | 6 | 22 |
| α-helix | 1188-1204 | 17 | |
Chain DB: 44 helices, 39 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 26-39 | 14 | 103 |
| β-strand | 44-55 | 12 | 103 |
| β-strand | 63-65 | 3 | 104 |
| β-strand | 66 | 1 | 105 |
| β-strand | 71-77 | 7 | 103 |
| β-strand | 80-81 | 2 | 103 |
| β-strand | 83-86 | 4 | 104 |
| α-helix | 103-116 | 14 | |
| β-strand | 126-129 | 4 | 104 |
| α-helix | 130-131 | 2 | |
| α-helix | 132-134 | 3 | |
| α-helix | 135-138 | 4 | |
| β-strand | 144-152 | 9 | 103 |
| β-strand | 160-162 | 3 | 106 |
| α-helix | 170-173 | 4 | |
| β-strand | 176-178 | 3 | 106 |
| α-helix | 186-188 | 3 | |
| β-strand | 192 | 1 | 105 |
| β-strand | 200-209 | 10 | 103 |
| β-strand | 213-216 | 4 | 106 |
| β-strand | 219-225 | 7 | 103 |
| α-helix | 226 | 1 | |
| β-strand | 231-242 | 12 | 103 |
| α-helix | 244-246 | 3 | |
| β-strand | 249-252 | 4 | 106 |
| α-helix | 254 | 1 | |
| β-strand | 255-258 | 4 | 107 |
| β-strand | 265-269 | 5 | 107 |
| α-helix | 274-280 | 7 | |
| α-helix | 284-294 | 11 | |
| α-helix | 298-299 | 2 | |
| β-strand | 304-309 | 6 | 107 |
| β-strand | 316-319 | 4 | 107 |
| β-strand | 322-326 | 5 | 107 |
| α-helix | 327-329 | 3 | |
| α-helix | 339-352 | 14 | |
| α-helix | 353-358 | 6 | |
| β-strand | 360-361 | 2 | 108 |
| α-helix | 367-408 | 42 | |
| α-helix | 438-440 | 3 | |
| α-helix | 443-463 | 21 | |
| α-helix | 466-483 | 18 | |
| α-helix | 493-495 | 3 | |
| β-strand | 497-498 | 2 | 108 |
| α-helix | 500-511 | 12 | |
| α-helix | 516-522 | 7 | |
| β-strand | 528 | 1 | 109 |
| β-strand | 529-538 | 10 | 110 |
| β-strand | 543-550 | 8 | 110 |
| β-strand | 557 | 1 | 109 |
| β-strand | 563-570 | 8 | 111 |
| β-strand | 573-580 | 8 | 111 |
| β-strand | 587-591 | 5 | 110 |
| α-helix | 596-599 | 4 | |
| α-helix | 603 | 1 | |
| β-strand | 604-605 | 2 | 112 |
| β-strand | 611-612 | 2 | 112 |
| β-strand | 625-630 | 6 | 111 |
| β-strand | 638-643 | 6 | 110 |
| α-helix | 646-655 | 10 | |
| α-helix | 659-668 | 10 | |
| α-helix | 669-671 | 3 | |
| α-helix | 675-685 | 11 | |
| β-strand | 688 | 1 | 113 |
| β-strand | 690 | 1 | 113 |
| α-helix | 692-708 | 17 | |
| α-helix | 717 | 1 | |
| α-helix | 720-726 | 7 | |
| β-strand | 728 | 1 | 114 |
| β-strand | 736 | 1 | 114 |
| α-helix | 737-739 | 3 | |
| α-helix | 746-757 | 12 | |
| α-helix | 769-780 | 12 | |
| α-helix | 792-804 | 13 | |
| α-helix | 807-809 | 3 | |
| α-helix | 824-842 | 19 | |
| α-helix | 849-863 | 15 | |
| α-helix | 872-877 | 6 | |
| α-helix | 884-900 | 17 | |
| α-helix | 904-915 | 12 | |
| α-helix | 920-932 | 13 | |
| α-helix | 947-958 | 12 | |
| α-helix | 967-979 | 13 | |
Chain Dc: 5 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 2-15 | 14 | |
| α-helix | 17-20 | 4 | |
| β-strand | 25-27 | 3 | 8 |
| α-helix | 28-55 | 28 | |
| α-helix | 63-73 | 11 | |
| α-helix | 77-86 | 10 | |
| β-strand | 101 | 1 | 9 |
75 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| DNA (228-mer) | X | DNA | 228 | synthetic construct | |
| DNA (228-mer) | Y | DNA | 228 | synthetic construct | |
| Transcription initiation factor IIB | BA | protein | 316 | Homo sapiens | Q00403 (AlphaFold model) |
| Transcription initiation factor TFIID subunit 1 | DA | protein | 1872 | Homo sapiens | P21675 (AlphaFold model) |
| General transcription factor IIE subunit 1 | EA | protein | 439 | Homo sapiens | P29083 (AlphaFold model) |
| General transcription factor IIF subunit 1 | FA | protein | 517 | Homo sapiens | P35269 (AlphaFold model) |
| General transcription and DNA repair factor IIH helicase subunit XPD | HA | protein | 760 | Homo sapiens | P18074 |
| Histone H3 | NA, NE | protein | 136 | Xenopus laevis | A0A310TTQ1 |
| DNA-directed RNA polymerase subunit | PA | protein | 1970 | Sus scrofa | A0A8D1DPV6 |
| Transcription initiation factor TFIID subunit 2 | DB | protein | 1199 | Homo sapiens | Q6P1X5 |
| Transcription initiation factor IIE subunit beta | EB | protein | 291 | Homo sapiens | P29084 |
| General transcription factor IIF subunit 2 | FB | protein | 249 | Homo sapiens | P13984 |
65 more molecules are not listed.
Sequence of entity 1 (X), FASTA
>8GXQ_1 DNA (228-mer) (chains X)
GAAGGGCGCCTATAAAAGGGGGTGGGGGCGCGTTCGTCCGCACGGCCGATCGAACACTCG
AGCCGAGCAGACGTGCCTACGCTGGAGAATCCCGGTGCCGAGGCCGCTCAATTGGTCGTA
GACAGCTCTAGCACCGCTTAAACGCACGTACGCGCTGTCCCCCGCGTTTTAACCGCCAAG
GGGATTACTCCCTAGTCTCCAGGCACGTGTCACATATATACATCCTGT
Sequence of entity 2 (Y), FASTA
>8GXQ_2 DNA (228-mer) (chains Y)
ACAGGATGTATATATGTGACACGTGCCTGGAGACTAGGGAGTAATCCCCTTGGCGGTTAA
AACGCGGGGGACAGCGCGTACGTGCGTTTAAGCGGTGCTAGAGCTGTCTACGACCAATTG
AGCGGCCTCGGCACCGGGATTCTCCAGCGTAGGCACGTCTGCTCGGCTCGAGTGTTCGAT
CGGCCGTGCGGACGAACGCGCCCCCACCCCCTTTTATAGGCGCCCTTC
Sequence of entity 3 (BA), FASTA
>8GXQ_3 Transcription initiation factor IIB (chains BA)
MASTSRLDALPRVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKA
TKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKE
ITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEI
CAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLCLPKQVQMAATHIARKAV
ELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLF
PTDFKFDTPVDKLPQL
Sequence of entity 4 (DA), FASTA
>8GXQ_4 Transcription initiation factor TFIID subunit 1 (chains DA)
MGPGCDLLLRTAATITAAAIMSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDD
ECKKHLAGLGALGLGSLITELTANEELTGTDGALVNDEGWVRSTEDAVDYSDINEVAEDE
SRRYQQTMGSLQPLCHSDYDEDDYDADCEDIDCKLMPPPPPPPGPMKKDKDQDSITGEKV
DFSSSSDSESEMGPQEATQAESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLR
FLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQEVECSVESEVSQKSLWNYDYAPPP
PPEQCLSDDEITMMAPVESKFSQSTGDIDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGS
GFDYGFKLRKTEHEPVIKSRMIEEFRKLEENNGTDLLADENFLMVTQLHWEDDIIWDGED
VKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEDLVYGRW
EDNIIWDAQAMPRLLEPPVLTLDPNDENLILEIPDEKEEATSNSPSKESKKESSLKKSRI
LLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQ
PFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGG
EMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYG
ETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIF
VVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSES
SIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKD
AGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCG
EGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKL
SRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSST
EVLSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMLLAAGSAA
SGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTT
KDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDL
KLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEG
TKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTD
PMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYP
SREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLD
DDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVNPMDLETIRKNISKH
KYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICT
AKEAALEEAELESLDPMTPGPYTPQPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPSAT
PEKQVTQEGEDGDGDLADEEEGTVQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSA
IQLSESGSDSDVGSGGIRPKQPRMLQENTRMDMENEESMMSYEGDGGEASHGLEDSNISY
GSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEEQRSGPSVLSQVHLSEDEEDSEDFH
SIAGDSDLDSDE
Sequence of entity 5 (EA), FASTA
>8GXQ_5 General transcription factor IIE subunit 1 (chains EA)
MADPDVLTEVPAALKRLAKYVIRGFYGIEHALALDILIRNSCVKEEDMLELLKFDRKQLR
SVLNNLKGDKFIKCRMRVETAADGKTTRHNYYFINYRTLVNVVKYKLDHMRRRIETDERD
STNRASFKCPVCSSTFTDLEANQLFDPMTGTFRCTFCHTEVEEDESAMPKKDARTLLARF
NEQIEPIYALLRETEDVNLAYEILEPEPTEIPALKQSKDHAATTAGAASLAGGHHREAWA
TKGPSYEDLYTQNVVINMDDQEDLHRASLEGKSAKERPIWLRESTVQGAYGSEDMKEGGI
DMDAFQEREEGHAGPDDNEEVMRALLIHEKKTSSAMAGSVGAAAPVTAANGSDSESETSE
SDDDSPPRPAAVAVHKREEDEEEDDEFEEVADDPIVMVAGRPFSYSEVSQRPELVAQMTP
EEKEAYIAMGQRMFEDLFE
Sequence of entity 6 (FA), FASTA
>8GXQ_6 General transcription factor IIF subunit 1 (chains FA)
MAALGPSSQNVTEYVVRVPKNTTKKYNIMAFNAADKVNFATWNQARLERDLSNKKIYQEE
EMPESGAGSEFNRKLREEARRKKYGIVLKEFRPEDQPWLLRVNGKSGRKFKGIKKGGVTE
NTSYYIFTQCPDGAFEAFPVHNWYNFTPLARHRTLTAEEAEEEWERRNKVLNHFSIMQQR
RLKDQDQDEDEEEKEKRGRRKASELRIHDLEDDLEMSSDASDASGEEGGRVPKAKKKAPL
AKGGRKKKKKKGSDDEAFEDSDDGDFEGQEVDYMSDGSSSSQEEPESKAKAPQQEEGPKG
VDEQSDSSEESEEEKPPEEDKEEEEEKKAPTPQEKKRRKDSSEESDSSEESDIDSEASSA
LFMAKKKTPPKRERKPSGGSSRGNSRPGTPSAEGGSTSSTLRAAASKLEQGKRVSEMPAA
KRLRLDTGPQSLSGKSTPQPPSGKTTPNSGDVQVTEDAVRRYLTRKPMTTKDLLKKFQTK
KTGLSSEQTVNVLAQILKRLNPERKMINDKMHFSLKE
Sequence of entity 7 (HA), FASTA
>8GXQ_7 General transcription and DNA repair factor IIH helicase subunit XPD (chains HA)
MKLNVDGLLVYFPYDYIYPEQFSYMRELKRTLDAKGHGVLEMPSGTGKTVSLLALIMAYQ
RAYPLEVTKLIYCSRTVPEIEKVIEELRKLLNFYEKQEGEKLPFLGLALSSRKNLCIHPE
VTPLRFGKDVDGKCHSLTASYVRAQYQHDTSLPHCRFYEEFDAHGREVPLPAGIYNLDDL
KALGRRQGWCPYFLARYSILHANVVVYSYHYLLDPKIADLVSKELARKAVVVFDEAHNID
NVCIDSMSVNLTRRTLDRCQGNLETLQKTVLRIKETDEQRLRDEYRRLVEGLREASAARE
TDAHLANPVLPDEVLQEAVPGSIRTAEHFLGFLRRLLEYVKWRLRVQHVVQESPPAFLSG
LAQRVCIQRKPLRFCAERLRSLLHTLEITDLADFSPLTLLANFATLVSTYAKGFTIIIEP
FDDRTPTIANPILHFSCMDASLAIKPVFERFQSVIITSGTLSPLDIYPKILDFHPVTMAT
FTMTLARVCLCPMIIGRGNDQVAISSKFETREDIAVIRNYGNLLLEMSAVVPDGIVAFFT
SYQYMESTVASWYEQGILENIQRNKLLFIETQDGAETSVALEKYQEACENGRGAILLSVA
RGKVSEGIDFVHHYGRAVIMFGVPYVYTQSRILKARLEYLRDQFQIRENDFLTFDAMRHA
AQCVGRAIRGKTDYGLMVFADKRFARGDKRGKLPRWIQEHLTDANLNLTVDEGVQVAKYF
LRQMAQPFHREDQLGLSLLSLEQLESEETLKRIEQIAQQL
Sequence of entity 8 (NA, NE), FASTA
>8GXQ_8 Histone H3 (chains NA, NE)
MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTE
LLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEASEAYLVALFEDTNLCAIHAKRVTI
MPKDIQLARRIRGERA
Sequence of entity 9 (PA), FASTA
>8GXQ_9 DNA-directed RNA polymerase subunit (chains PA)
MHGGGPPSGDSACPLRTIKRVQFGVLSPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDP
RQGVIERTGRCQTCAGNMTECPGHFGHIELAKPVFHVGFLVKTMKVLRCVCFFCSKLLVD
SNNPKIKDILAKSKGQPKKRLTHVYDLCKGKNICEGGEEMDNKFGVEQPEGDEDLTKEKG
HGGCGRYQPRIRRSGLELYAEWKHVNEDSQEKKILLSPERVHEIFKRISDEECFVLGMEP
RYARPEWMIVTVLPVPPLSVRPAVVMQGSARNQDDLTHKLADIVKINNQLRRNEQNGAAA
HVIAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPLKSLKQRLKGKEGRVRGNLMGKRVD
FSARTVITPDPNLSIDQVGVPRSIAANMTFAEIVTPFNIDRLQELVRRGNSQYPGAKYII
RDNGDRIDLRFHPKPSDLHLQTGYKVERHMCDGDIVIFNRQPTLHKMSMMGHRVRILPWS
TFRLNLSVTTPYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQD
TLTAVRKFTKRDVFLERGEVMNLLMFLSTWDGKVPQPAILKPRPLWTGKQIFSLIIPGHI
NCIRTHSTHPDDEDSGPYKHISPGDTKVVVENGELIMGILCKKSLGTSAGSLVHISYLEM
GHDITRLFYSNIQTVINNWLLIEGHTIGIGDSIADSKTYQDIQNTIKKAKQDVIEVIEKA
HNNELEPTPGNTLRQTFENQVNRILNDARDKTGSSAQKSLSEYNNFKSMVVSGAKGSKIN
ISQVIAVVGQQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHA
MGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGLAGES
VEFQNLATLKPSNKAFEKKFRFDYTNERALRRTLQEDLVKDVLSNAHIQNELEREFERMR
EDREVLRVIFPTGDSKVVLPCNLLRMIWNAQKIFHINPRLPSDLHPIKVVEGVKELSKKL
VIVNGDDPLSRQAQENATLLFNIHLRSTLCSRRMAEEFRLSGEAFDWLLGEIESKFNQAI
AHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKELINISKKPKTPSLT
VFLLGQSARDAERAKDILCRLEHTTLRKVTANTAIYYDPNPQSTVVAEDQEWVNVYYEMP
DFDVARISPWLLRVELDRKHMTDRKLTMEQIAEKINAGFGDDLNCIFNDDNAEKLVLRIR
IMNSDENKMQEEEEVVDKMDDDVFLRCIESNMLTDMTLQGIEQISKVYMHLPQTDNKKKI
IITEDGEFKALQEWILETDGVSLMRVLSEKDVDPVRTTSNDIVEIFTVLGIEAVRKALER
ELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGVNRQDTGPLMKCSFEETVDVLM
EAAAHGESDPMKGVSENIMLGQLAPAGTGCFDLLLDAEKCKYGMEIPTNIPGLGAAGPTG
MFFGSAPSPMGGISPAMTPWNQGATPAYGAWSPSVGSGMTPGAAGFSPSAASDASGFSPG
YSPAWSPTPGSPGSPGPSSPYIPSPGGAMSPSYSPTSPAYEPRSPGGYTPQSPSYSPTSP
SYSPTSPSYSPTSPNYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSP
TSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPS
YSPTSPNYSPTSPNYTPTSPSYSPTSPSYSPTSPNYTPTSPNYSPTSPSYSPTSPSYSPT
SPSYSPSSPRYTPQSPTYTPSSPSYSPSSPSYSPTSPKYTPTSPSYSPSSPEYTPTSPKY
SPTSPKYSPTSPKYSPTSPTYSPTTPKYSPTSPTYSPTSPVYTPTSPKYSPTSPTYSPTS
PKYSPTSPTYSPTSPKGSTYSPTSPGYSPTSPTYSLTSPAISPDDSDEEN
Sequence of entity 10 (DB), FASTA
>8GXQ_10 Transcription initiation factor TFIID subunit 2 (chains DB)
MPLTGVEPARMNRKKGDKGFESPRPYKLTHQVVCINNINFQRKSVVGFVELTIFPTVANL
NRIKLNSKQCRIYRVRINDLEAAFIYNDPTLEVCHSESKQRNLNYFSNAYAAAVSAVDPD
AGNGELCIKVPSELWKHVDELKVLKIHINFSLDQPKGGLHFVVPSVEGSMAERGAHVFSC
GYQNSTRFWFPCVDSYSELCTWKLEFTVDAAMVAVSNGDLVETVYTHDMRKKTFHYMLTI
PTAASNISLAIGPFEILVDPYMHEVTHFCLPQLLPLLKHTTSYLHEVFEFYEEILTCRYP
YSCFKTVFIDEAYVEVAAYASMSIFSTNLLHSAMIIDETPLTRRCLAQSLAQQFFGCFIS
RMSWSDEWVLKGISGYIYGLWMKKTFGVNEYRHWIKEELDKIVAYELKTGGVLLHPIFGG
GKEKDNPASHLHFSIKHPHTLSWEYYSMFQCKAHLVMRLIENRISMEFMLQVFNKLLSLA
STASSQKFQSHMWSQMLVSTSGFLKSISNVSGKDIQPLIKQWVDQSGVVKFYGSFAFNRK
RNVLELEIKQDYTSPGTQKYVGPLKVTVQELDGSFNHTLQIEENSLKHDIPCHSKSRRNK
KKKIPLMNGEEVDMDLSAMDADSPLLWIRIDPDMSVLRKVEFEQADFMWQYQLRYERDVV
AQQESILALEKFPTPASRLALTDILEQEQCFYRVRMSACFCLAKIANSMVSTWTGPPAMK
SLFTRMFCCKSCPNIVKTNNFMSFQSYFLQKTMPVAMALLRDVHNLCPKEVLTFILDLIK
YNDNRKNKFSDNYYRAEMIDALANSVTPAVSVNNEVRTLDNLNPDVRLILEEITRFLNME
KLLPSYRHTITVSCLRAIRVLQKNGHVPSDPALFKSYAEYGHFVDIRIAALEAVVDYTKV
DRSYEELQWLLNMIQNDPVPYVRHKILNMLTKNPPFTKNMESPLCNEALVDQLWKLMNSG
TSHDWRLRCGAVDLYFTLFGLSRPSCLPLPELGLVLNLKEKKAVLNPTIIPESVAGNQEA
ANNPSSHPQLVGFQNPFSSSQDEEEIDMDTVHDSQAFISHHLNMLERPSTPGLSKYRPAS
SRSALIPQHSAGCDSTPTTKPQWSLELARKGTGKEQAPLEMSMHPAASAPLSVFTKESTA
SKHSDHHHHHHHEHKKKKKKHKHKHKHKHKHDSKEKDKEPFTFSSPASGRSIRSPSLSD
Sequence of entity 11 (EB), FASTA
>8GXQ_11 Transcription initiation factor IIE subunit beta (chains EB)
MDPSLLRERELFKKRALSTPVVEKRSASSESSSSSSKKKKTKVEHGGSSGSKQNSDHSNG
SFNLKALSGSSGYKFGVLAKIVNYMKTRHQRGDTHPLTLDEILDETQHLDIGLKQKQWLM
TEALVNNPKIEVIDGKYAFKPKYNVRDKKALLRLLDQHDQRGLGGILLEDIEEALPNSQK
AVKALGDQILFVNRPDKKKILFFNDKSCQFSVDEEFQKLWRSVTVDSMDEEKIEEYLKRQ
GISSMQESGPKKVAPIQRRKKPASQKKRRFKTHNEHLAGVLKDYSDITSSK
Sequence of entity 12 (FB), FASTA
>8GXQ_12 General transcription factor IIF subunit 2 (chains FB)
MAERGELDLTGAKQNTGVWLVKVPKYLSQQWAKASGRGEVGKLRIAKTQGRTEVSFTLNE
DLANIHDIGGKPASVSAPREHPFVLQSVGGQTLTVFTESSSDKLSLEGIVVQRAECRPAA
SENYMRLKRLQIEESSKPVRLSQQLDKVVTTNYKPVANHQYNIEYERKKKEDGKRARADK
QHVLDMLFSAFEKHQYYNLKDLVDITKQPVVYLKEILKEIGVQNVKGIHKNTWELKPEYR
HYQGEEKSD
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| MG | Magnesium ion | Mg | 1 |
| SF4 | Iron/sulfur cluster | Fe4 S4 | 1 |
| ZN | Zinc ion | Zn | 19 |
Primary citation
Structures of +1 nucleosome-bound PIC-Mediator complex. Chen, X., Wang, X., Liu, W. et al. Science (2022) 378:62-68. DOI 10.1126/science.abn8131 · PubMed
Other PDB entries of the same protein (UniProt Q00403 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 7NVU 2.5 Å, RNA polymerase II core pre-initiation complex with open promoter DNA
- 1C9B 2.65 Å, Crystal structure of a human tbp core domain-human tfiib core domain complex bound to an…
- 1VOL 2.7 Å, Tfiib (human core domain)/tbp (a.thaliana)/tata element ternary complex
- 7NVS 2.8 Å, RNA polymerase II core pre-initiation complex with closed promoter DNA in proximal…
- 7NVT 2.9 Å, RNA polymerase II core pre-initiation complex with closed promoter DNA in distal position
- 8S52 2.9 Å, RNA polymerase II core initially transcribing complex with an ordered RNA of 10 nt
- 7ZWD 3.0 Å, Structure of SNAPc containing Pol II pre-initiation complex bound to U5 snRNA promoter…
- 7ZX8 3.0 Å, Structure of SNAPc containing Pol II pre-initiation complex bound to U1 snRNA promoter…
- 8S51 3.1 Å, RNA polymerase II core initially transcribing complex with an ordered RNA of 8 nt
- 7ZWC 3.2 Å, Structure of SNAPc:TBP-TFIIA-TFIIB sub-complex bound to U5 snRNA promoter
- 7EGB 3.3 Å, TFIID-based holo PIC on SCP promoter
- 5WH1 3.39 Å, Apo form of the C-terminal region of human Transcription Factor IIB
Browse structure collections
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