2MRE: Rad18-UBZ/ubiquitin complex

NMR structure of the Rad18-UBZ/ubiquitin complex. Determined by solution NMR. Released 22 Oct 2014.

Method: Solution NMR
Organism: Homo sapiens
Chains: 2
Atoms: 873
Mol. weight: 12.55 kDa
Ligands: ZN
Released: 22 Oct 2014

Explore 2MRE in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2MRE contains 4 α-helices and 7 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 2 helices, 5 β-strands

Element	Residues	Length	Sheet
β-strand	1-7	7	1
β-strand	12-17	6	1
α-helix	23-33	11
β-strand	41-45	5	1
β-strand	48-49	2	1
α-helix	57-59	3
β-strand	66-71	6	1

Chain B: 2 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	199-200	2
β-strand	201-204	4	2
β-strand	209-212	4	2
α-helix	213-223	11

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Polyubiquitin-C	A	protein	79	Homo sapiens	P0CG48 (AlphaFold model)
E3 ubiquitin-protein ligase RAD18	B	protein	33	Homo sapiens	Q9NS91 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>2MRE_1 Polyubiquitin-C (chains A)
GSHMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLS
DYNIQKESTLHLVLRLRGG

Sequence of entity 2 (B), FASTA

>2MRE_2 E3 ubiquitin-protein ligase RAD18 (chains B)
GSRQVTKVDCPVCGVNIPESHINKHLDSCLSRE

Ligands and cofactors

ID	Name	Formula	Copies
ZN	Zinc ion	Zn	1

Primary citation

NMR Structure of the Human Rad18 Zinc Finger in Complex with Ubiquitin Defines a Class of UBZ Domains in Proteins Linked to the DNA Damage Response. Rizzo, A.A., Salerno, P.E., Bezsonova, I. et al. Biochemistry (2014) 53:5895-5906. DOI 10.1021/bi500823h · PubMed

Other PDB entries of the same protein (UniProt P0CG48 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6Q00 0.85 Å, TDP2 UBA Domain Bound to Ubiquitin at 0.85 Angstroms Resolution, Crystal Form 1
1OGW 1.32 Å, Synthetic Ubiquitin with fluoro-Leu at 50 and 67
5NL4 1.32 Å, Crystal structure of Zn1.3-E16V human ubiquitin (hUb) mutant adduct, from a solution 35…
2GBJ 1.35 Å, Crystal Structure of the 9-10 8 Glycine Insertion Mutant of Ubiquitin.
4HK2 1.4 Å, U7Ub25.2540
9FJ3 1.4 Å, Structure of ubiquitin bound of coiled-coil UIM form 2
1XD3 1.45 Å, Crystal structure of UCHL3-UbVME complex
4IUM 1.45 Å, Equine arteritis virus papain-like protease 2 (PLP2) covalently bound to ubiquitin
7UV5 1.45 Å, The crystal structure of Papain-Like Protease of SARS CoV-2, C111S/D286N mutant, in…
5NLF 1.5 Å, Crystal structure of Zn2.7-E16V human ubiquitin (hUb) mutant adduct, from a solution 100…
9F6G 1.5 Å, Human USP30 chimera bound to Ubiquitin-PA
9OVX 1.5 Å, Crystal structure of ubiquitin K27M mutant

Browse structure collections

About this viewer

MolViewer shows 2MRE directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.