2SNS: Thermonuclease precursor

Staphylococcal nuclease. Proposed mechanism of action based on structure of enzyme-thymidine 3(PRIME),5(PRIME)-biphosphate-calcium ion complex at 1.5-Å resolution. Determined by X-ray diffraction at 1.5 Å resolution. Released 29 Jul 1982.

Method: X-ray diffraction
Resolution: 1.5 Å
Organism: Staphylococcus aureus
Chains: 1
Atoms: 1,151
Mol. weight: 17.28 kDa
Ligands: THP, CA
Released: 29 Jul 1982

Explore 2SNS in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2SNS contains 4 α-helices and 12 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 4 helices, 12 β-strands

Element	Residues	Length	Sheet
β-strand	9-12	4	1
β-strand	14-17	4	2
β-strand	22-25	4	2
β-strand	32-36	5	2
β-strand	40	1	3
α-helix	57-59	3
α-helix	62-67	6
β-strand	72-75	4	1
β-strand	76	1	2
β-strand	82	1	2
β-strand	88-90	3	2
β-strand	93-94	2	1
β-strand	97-98	2	1
α-helix	99-105	7
β-strand	110	1	3
α-helix	122-134	13

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Thermonuclease precursor	A	protein	149	Staphylococcus aureus	P00644 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>2SNS_1 THERMONUCLEASE PRECURSOR (chains A)
ATSTKKLHKEPATLIKAIDGDTVKLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASA
FTKKMVENAKKIEVEFNKGQRTDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNT
HEQHLRKSEAQAKKEKLNIWSENDADSGQ

Ligands and cofactors

ID	Name	Formula	Copies
THP	Thymidine-3',5'-diphosphate	C10 H16 N2 O11 P2	1
CA	Calcium ion	Ca	1

Primary citation

STAPHYLOCOCCAL NUCLEASE. PROPOSED MECHANISM OF ACTION BASED ON STRUCTURE OF ENZYME-THYMIDINE 3(PRIME),5(PRIME)-BIPHOSPHATE-CALCIUM ION COMPLEX AT 1.5-ANGSTROMS RESOLUTION. Cotton, F.A., Hazen Jr., E.E., Legg, M.J. Int Congr Ser (1977). PubMed

Other PDB entries of the same protein (UniProt P00644 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4EQP 1.35 Å, Crystal structure of Staphylococcal nuclease variant Delta+PHS I72D at cryogenic…
4HMJ 1.35 Å, Crystal structure of Staphylococcal nuclease variant Delta+PHS L36D at cryogenic…
3SXH 1.4 Å, Crystal structure of Staphylococcal nuclease variant Delta+PHS I92AL103A at cryogenic…
3TME 1.4 Å, Crystal structure of Staphylococcal nuclease variant Delta+NVIAGLA V23E at cryogenic…
4KY5 1.4 Å, Crystal structure of Staphylococcal nuclease variant Delta+PHS V23D at pH 7 and…
4N9P 1.4 Å, Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/L25A/V99T at…
4DFA 1.4 Å, Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/L36A at cryogenic…
3SR1 1.45 Å, Crystal structure of Staphylococcal nuclease variant Delta+PHS A69G bound to Ca2+ and…
5JOB 1.45 Å, Crystal structure of Staphylococcal nuclease variant Delta+PHS L25K/I92A at cryogenic…
4DGZ 1.47 Å, Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/L125A at cryogenic…
3SHL 1.48 Å, Crystal structure of Staphylococcal nuclease variant Delta+PHS V74KL25A at cryogenic…
4NDX 1.5 Å, Crystal structure of Staphylococcal nuclease variant Delta+PHS I92N at cryogenic…

Browse structure collections

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