4KY5: Thermonuclease

Crystal structure of Staphylococcal nuclease variant Delta+PHS V23D at pH 7 and cryogenic temperature. Determined by X-ray diffraction at 1.4 Å resolution. Released 12 Jun 2013.

Method
X-ray diffraction
Resolution
1.4 Å
Organism
Staphylococcus aureus
Chains
1
Atoms
1,254
Mol. weight
16.6 kDa
Ligands
THP, CA
Released
12 Jun 2013

Explore 4KY5 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4KY5 contains 5 α-helices and 9 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 5 helices, 9 β-strands

ElementResiduesLengthSheet
β-strand9-19111
β-strand22-2761
β-strand30-3671
β-strand39-4022
α-helix41-433
α-helix55-6814
β-strand72-7541
β-strand8211
β-strand88-9471
β-strand97-9821
α-helix99-1057
β-strand110-11122
α-helix122-13413
α-helix138-1403

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
ThermonucleaseAprotein143Staphylococcus aureusP00644 (AlphaFold model)
Sequence of entity 1 (A), FASTA
>4KY5_1 Thermonuclease (chains A)
ATSTKKLHKEPATLIKAIDGDTDKLMYKGQPMTFRLLLVDTPEFNEKYGPEASAFTKKMV
ENAKKIEVEFDKGQRTDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKGNNTHEQLLR
KAEAQAKKEKLNIWSEDNADSGQ

Ligands and cofactors

IDNameFormulaCopies
THPThymidine-3',5'-diphosphateC10 H16 N2 O11 P21
CACalcium ionCa1

Primary citation

Crystal structure of Staphylococcal nuclease variant Delta+PHS V23D at pH 7 and cryogenic temperature. Robinson, A.C., Schlessman, J.L., Heroux, A. et al. To be published.

Other PDB entries of the same protein (UniProt P00644 (AlphaFold model), which also has an AlphaFold model), best resolution first:

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