2UXX: Human LSD1 Histone Demethylase-CoREST

Human LSD1 Histone Demethylase-CoREST in complex with an FAD- tranylcypromine adduct. Determined by X-ray diffraction at 2.74 Å resolution. Released 21 Aug 2007.

Method: X-ray diffraction
Resolution: 2.74 Å
Organism: HOMO SAPIENS
Chains: 2
Atoms: 6,379
Mol. weight: 101.69 kDa
Ligands: FAJ
Released: 21 Aug 2007

Explore 2UXX in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2UXX contains 41 α-helices and 40 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 33 helices, 38 β-strands

Element	Residues	Length	Sheet
α-helix	173-180	8
α-helix	190-195	6
α-helix	197-201	5
α-helix	204-223	20
β-strand	227	1	1
α-helix	231-236	6
α-helix	238-239	2
α-helix	246-258	13
β-strand	268	1	1
α-helix	272-274	3
β-strand	280-284	5	2
β-strand	287	1	3
α-helix	288-299	12
β-strand	303-307	5	2
β-strand	314	1	3
β-strand	319-322	4	4
β-strand	325-328	4	4
β-strand	333-334	2	5
β-strand	338	1	6
α-helix	342-348	7
β-strand	353-355	3	5
α-helix	356-357	2
β-strand	362-363	2	7
α-helix	368	1
β-strand	369	1	7
α-helix	370-371	2
α-helix	372-394	23
β-strand	400-401	2	8
β-strand	404-405	2	8
α-helix	406	1
β-strand	407	1	9
α-helix	408-466	59
α-helix	469	1
α-helix	474-513	40
α-helix	523-539	17
β-strand	547	1	10
β-strand	548	1	9
α-helix	556-558	3
α-helix	559-560	2
β-strand	561	1	6
β-strand	565-567	3	5
α-helix	573-578	6
β-strand	583-585	3	2
β-strand	588-595	8	11
β-strand	600-606	7	11
β-strand	613-618	6	11
β-strand	620-623	4	2
α-helix	627-631	5
β-strand	638-640	3	11
α-helix	642-644	3
α-helix	645-653	9
β-strand	655-656	2	12
β-strand	660-665	6	7
β-strand	677-680	4	7
β-strand	693-695	3	7
β-strand	702-707	6	7
α-helix	710-715	6
α-helix	720-735	16
α-helix	741-743	3
β-strand	745-748	4	7
β-strand	762-763	2	12
β-strand	765	1	10
β-strand	766	1	13
β-strand	768	1	13
α-helix	771-777	7
β-strand	780	1	2
α-helix	782-784	3
α-helix	792-794	3
β-strand	796-798	3	2
α-helix	801-803	3
α-helix	811-830	20

Chain B: 8 helices, 2 β-strands

Element	Residues	Length	Sheet
β-strand	312	1	14
β-strand	314	1	14
α-helix	318-324	7
α-helix	330-361	32
α-helix	368-370	3
α-helix	383-384	2
α-helix	385-398	14
α-helix	402-409	8
α-helix	414-423	10
α-helix	430-438	9

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Lysine-specific histone demethylase 1	A	protein	666	HOMO SAPIENS	O60341 (AlphaFold model)
Rest corepressor 1	B	protein	235	HOMO SAPIENS	Q9UKL0 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>2UXX_1 LYSINE-SPECIFIC HISTONE DEMETHYLASE 1 (chains A)
PSGVEGAAFQSRLPHDRMTSQEAACFPDIISGPQQTQKVFLFIRNRTLQLWLDNPKIQLT
FEATLQQLEAPYNSDTVLVHRVHSYLERHGLINFGIYKRIKPLPTKKTGKVIIIGSGVSG
LAAARQLQSFGMDVTLLEARDRVGGRVATFRKGNYVADLGAMVVTGLGGNPMAVVSKQVN
MELAKIKQKCPLYEANGQAVPKEKDEMVEQEFNRLLEATSYLSHQLDFNVLNNKPVSLGQ
ALEVVIQLQEKHVKDEQIEHWKKIVKTQEELKELLNKMVNLKEKIKELHQQYKEASEVKP
PRDITAEFLVKSKHRDLTALCKEYDELAETQGKLEEKLQELEANPPSDVYLSSRDRQILD
WHFANLEFANATPLSTLSLKHWDQDDDFEFTGSHLTVRNGYSCVPVALAEGLDIKLNTAV
RQVRYTASGCEVIAVNTRSTSQTFIYKCDAVLCTLPLGVLKQQPPAVQFVPPLPEWKTSA
VQRMGFGNLNKVVLCFDRVFWDPSVNLFGHVGSTTASRGELFLFWNLYKAPILLALVAGE
AAGIMENISDDVIVGRCLAILKGIFGSSAVPQPKETVVSRWRADPWARGSYSYVAAGSSG
NDYDLMAQPITPGPSIPGAPQPIPRLFFAGEHTIRNYPATVHGALLSGLREAGRIADQFL
GAMYTL

Sequence of entity 2 (B), FASTA

>2UXX_2 REST COREPRESSOR 1 (chains B)
MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSEFGRPTETVPQVKKEKHSTQAKNRAK
RKPPKGMFLSQEDVEAVSANATAATTVLRQLDMELVSVKRQIQNIKQTNSALKEKLDGGI
EPYRLPEVIQKCNARWTTEEQLLAVQAIRKYGRDFQAISDVIGNKSVVQVKNFFVNYRRR
FNIDEVLQEWEAEHGKEETNGPSNQKPVKSPDNSIKMPEEEDEAPVLDVRYASAS

Ligands and cofactors

ID	Name	Formula	Copies
FAJ	FAD-trans-2-Phenylcyclopropylamine Adduct	C36 H43 N9 O16 P2	1

Water and common crystallization additives (GOL, CL) are not listed.

Primary citation

Structural Basis for the Inhibition of the Lsd1 Histone Demethylase by the Antidepressant Trans-2-Phenylcyclopropylamine. Yang, M., Culhane, J.C., Szewczuk, L.M. et al. Biochemistry (2007) 46:8058. DOI 10.1021/BI700664Y · PubMed

Other PDB entries of the same protein (UniProt O60341 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6E1F 1.16 Å, Crystal structure of the SWIRM domain of human histone lysine-specific demethylase LSD1
5IT3 1.4 Å, Swirm domain of human Lsd1
5AFW 1.6 Å, Assembly of methylated LSD1 and CHD1 drives AR-dependent transcription and translocation
7JK7 1.96 Å, Crystal structure of Importin alpha 2 in complex with LSD1 NLS S111E mutant
7JJM 2.06 Å, Crystal structure of Importin alpha 2 in complex with LSD1 NLS
2Z3Y 2.25 Å, Crystal structure of Lysine-specific demethylase1
2Z5U 2.25 Å, Crystal structure of Lysine-specific histone demethylase 1
6KGO 2.25 Å, LSD1-S2157 five-membered ring adduct model
6KGP 2.25 Å, LSD1-S2157 N5 adduct model
7E0G 2.25 Å, Crystal structure of Lysine Specific Demethylase 1 (LSD1) with TAK-418, FAD-adduct
7XW8 2.28 Å, Crystal structure of Lysine Specific Demethylase 1 (LSD1) with TAK-418 distomer,…
2DW4 2.3 Å, Crystal structure of human LSD1 at 2.3 A resolution

Browse structure collections

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