2XNS: Human G alpha i1

Crystal Structure Of Human G alpha i1 Bound To A Designed Helical Peptide Derived From The Goloco Motif Of RGS14. Determined by X-ray diffraction at 3.41 Å resolution. Released 8 Jun 2011.

Method: X-ray diffraction
Resolution: 3.41 Å
Organism: HOMO SAPIENS
Chains: 4
Atoms: 5,862
Mol. weight: 85.52 kDa
Ligands: GDP, SRT
Released: 8 Jun 2011

Explore 2XNS in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2XNS contains 38 α-helices and 12 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chains A and B: 17 helices, 6 β-strands

Element	Residues	Length	Sheet
β-strand	33-40	8	1
α-helix	46-57	12
α-helix	63-90	28
α-helix	93-95	3
α-helix	100-111	12
α-helix	121-131	11
α-helix	134-140	7
α-helix	152-156	5
α-helix	159-162	4
α-helix	171-175	5
β-strand	185-191	7	1
β-strand	194-200	7	1
α-helix	208-215	8
β-strand	220-226	7	1
α-helix	227-231	5
α-helix	232-235	4
α-helix	242-254	13
β-strand	263-269	7	1
α-helix	271-277	7
α-helix	283-285	3
α-helix	296-308	13
β-strand	319-323	5	1
α-helix	329-344	16

Chains C and D: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	499-507	9
α-helix	521-531	11

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein g(i) subunit alpha-1	A, B	protein	327	HOMO SAPIENS	P63096 (AlphaFold model)
Regulator of G-protein signaling 14	C, D	protein	40	HOMO SAPIENS	O43566 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>2XNS_1 GUANINE NUCLEOTIDE-BINDING PROTEIN G(I) SUBUNIT ALPHA-1 (chains A, B)
SNAAREVKLLLLGAGESGKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRA
MGRLKIDFGDSARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQ
LNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSE
RKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILF
LNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCAT
DTKNVQFVFDAVTDVIIKNNLKDCGLF

Sequence of entity 2 (C, D), FASTA

>2XNS_2 REGULATOR OF G-PROTEIN SIGNALING 14 (chains C, D)
DIEGLVELLNRVQSSGAHDQRGLLSNEEVFRALRDFDRWF

Ligands and cofactors

ID	Name	Formula	Copies
GDP	Guanosine-5'-diphosphate	C10 H15 N5 O11 P2	2
SRT	S,r meso-tartaric acid	C4 H6 O6	2

Water and common crystallization additives (SO4) are not listed.

Primary citation

Computational Design of the Sequence and Structure of a Protein-Binding Peptide. Sammond, D.W., Bosch, D.E., Butterfoss, G.L. et al. J Am Chem Soc (2011) 133:4190. DOI 10.1021/JA110296Z · PubMed

Other PDB entries of the same protein (UniProt P63096 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6CRK 2.0 Å, Heterotrimeric G-protein in complex with an antibody fragment
3UMR 2.04 Å, Crystal structure of the G202D mutant of human G-alpha-i1
9P7Z 2.1 Å, NTSR1-Gi-NTS(8-13) Complex in the Canonical, AHD Open State (C-Open-Apo)
2OM2 2.2 Å, Crystal Structure Of Human G[alpha]i1 Bound To The Goloco Motif Of Rgs14
8YN9 2.3 Å, Cryo-EM structure of histamine H4 receptor in complex with histamine and Gi
9HYI 2.3 Å, DP81-bound serotonin 5-HT1A receptor - Gi Protein Complex
9O36 2.3 Å, CryoEM structure of mu-opioid receptor - Gi protein complex bound to fluornitrazene (FNZ)
9P80 2.3 Å, NTSR1-Gi-NTS(8-13) Complex in the Non-Canonical, AHD Open State (NC-Open-Apo)
8XXV 2.33 Å, Cryo-EM Structure of the Prostaglandin D2 Receptor 2-indomethacin Coupled to G Protein
3UMS 2.34 Å, Crystal structure of the G202A mutant of human G-alpha-i1
3ONW 2.38 Å, Structure of a G-alpha-i1 mutant with enhanced affinity for the RGS14 GoLoco motif.
20ZG 2.4 Å, Cryo-EM structure of the human neurotensin receptor 1 (hNTSR1)-Gi1 complex in the…

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