2XWT: Tsh receptor

Crystal structure of the tsh receptor in complex with a blocking type tshr autoantibody. Determined by X-ray diffraction at 1.9 Å resolution. Released 9 Mar 2011.

Method: X-ray diffraction
Resolution: 1.9 Å
Organism: HOMO SAPIENS
Chains: 3
Atoms: 5,932
Mol. weight: 74.79 kDa
Ligands: NAG
Released: 9 Mar 2011

Explore 2XWT in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2XWT contains 18 α-helices and 69 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 8 helices, 22 β-strands

Element	Residues	Length	Sheet
β-strand	3-6	4	1
α-helix	7-9	3
β-strand	10-12	3	2
β-strand	18-25	8	1
β-strand	33-39	7	2
β-strand	46-52	7	2
β-strand	56-59	4	2
β-strand	68-72	5	1
α-helix	73-75	3
β-strand	77-82	6	1
α-helix	84-86	3
β-strand	88-95	8	2
β-strand	102-103	2	2
β-strand	107-111	5	2
β-strand	117	1	3
α-helix	118-119	2
β-strand	120-124	5	4
β-strand	137-147	11	4
β-strand	148	1	3
β-strand	153-157	4	5
α-helix	162-164	3
β-strand	166	1	5
β-strand	171-173	3	4
α-helix	174-176	3
β-strand	177-178	2	4
β-strand	185-194	10	4
α-helix	195-197	3
β-strand	207-212	6	5
α-helix	213-215	3
β-strand	217-222	6	5

Chain B: 8 helices, 25 β-strands

Element	Residues	Length	Sheet
β-strand	4	1	6
β-strand	5	1	7
β-strand	9-13	4	8
β-strand	19-24	6	7
β-strand	34-38	5	8
β-strand	45-48	4	8
β-strand	49	1	9
β-strand	53	1	9
α-helix	54-55	2
β-strand	62-67	6	7
β-strand	70-75	6	7
α-helix	80-82	3
β-strand	84-92	9	8
β-strand	95B-98	4	8
β-strand	99	1	6
β-strand	102-106	5	8
α-helix	108-110	3
β-strand	111	1	10
α-helix	112-113	2
β-strand	114-118	5	11
α-helix	119-121	3
α-helix	122-126	5
β-strand	130-139	10	11
β-strand	140	1	10
β-strand	145-150	6	12
β-strand	153-155	3	12
β-strand	159-161	3	11
α-helix	162-164	3
β-strand	165-166	2	11
β-strand	173-181	9	11
α-helix	183-188	6
β-strand	192-198	7	12
β-strand	203-209	7	12

Chain C: 2 helices, 22 β-strands

Element	Residues	Length	Sheet
α-helix	29	1
β-strand	31-33	3	13
β-strand	38-41	4	13
β-strand	56-60	5	13
β-strand	66-67	2	14
β-strand	80-84	5	13
β-strand	91-92	2	14
α-helix	93	1
β-strand	97-98	2	15
β-strand	105-111	7	13
β-strand	116-117	2	14
β-strand	122-123	2	15
β-strand	130-136	7	13
β-strand	149	1	16
β-strand	153-159	7	13
β-strand	166-167	2	17
β-strand	176	1	16
β-strand	179-183	5	13
β-strand	191-192	2	17
β-strand	201-206	6	13
β-strand	215-216	2	17
β-strand	226	1	13
β-strand	230-232	3	13
β-strand	251-253	3	13

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Thyroid blocking human autoantibody K1-70 heavy chain	A	protein	221	HOMO SAPIENS
Thyroid blocking human autoantibody K1-70 light chain	B	protein	214	HOMO SAPIENS
Thyrotropin receptor	C	protein	239	HOMO SAPIENS	P16473 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>2XWT_1 THYROID BLOCKING HUMAN AUTOANTIBODY K1-70 HEAVY CHAIN (chains A)
EVQLVQSGAEVKKPGQSLKISCKASGYSLTDNWIGWVRQKPGKGLEWMGIIYPGDSDTRY
SPSFQGQVTISADKSINTAYLQWSSLKASDTAIYYCVGLDWNYNPLRYWGPGTLVTVSSA
STKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSG
LYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKS

Sequence of entity 2 (B), FASTA

>2XWT_2 THYROID BLOCKING HUMAN AUTOANTIBODY K1-70 LIGHT CHAIN (chains B)
QSVLTQPPSVSAAPGQKVTISCSGSSSDIGSNYVSWYQQFPGTAPKLLIYDNNKRPSAIP
DRFSGSKSGTSATLGITGLQTGDEADYYCGTWDSRLGIAVFGGGTQLTVLGQPKAAPSVT
LFPPSSEELQANKATLVCLVSDFYPGAVTVAWKADGSPVKVGVETTKPSKQSNNKYAASS
YLSLTPEQWKSHRSYSCRVTHEGSTVEKTVAPTE

Sequence of entity 3 (C), FASTA

>2XWT_3 THYROTROPIN RECEPTOR (chains C)
MGCSSPPCECHQEEDFRVTCKDIQRIPSLPPSTQTLKLIETHLRTIPSHAFSNLPNISRI
YVSIDVTLQQLESHSFYNLSKVTHIEIRNTRNLTYIDPDALKELPLLKFLGIFNTGLKMF
PDLTKVYSTDIFFILEITDNPYMTSIPVNAFQGLCNETLTLKLYNNGFTSVQGYAFNGTK
LDAVYLNKNKYLTVIDKDAFGGVYSGPSLLDVSQTSVTALPSKGLEHLKELIARNTWTL

Ligands and cofactors

ID	Name	Formula	Copies
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	2

Primary citation

Crystal Structure of the Tsh Receptor (Tshr) Bound to a Blocking-Type Tshr Autoantibody. Sanders, P., Young, S., Sanders, J. et al. J Mol Endocrinol (2011) 46:81. DOI 10.1530/JME-10-0127 · PubMed

Other PDB entries of the same protein (UniProt P16473 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7UTZ 2.4 Å, Human thyrotropin analog TR1402 bound to human Thyrotropin receptor in complex with…
3G04 2.55 Å, Crystal structure of the TSH receptor in complex with a thyroid-stimulating autoantibody
7XW6 2.78 Å, TSHR-Gs-M22 antibody-ML109 complex
7T9I 2.9 Å, Native human TSH bound to human Thyrotropin receptor in complex with miniGs399…
7T9N 2.9 Å, M22 Agonist Autoantibody bound to Human Thyrotropin receptor in complex with miniGs399…
7XW5 2.96 Å, TSHR-thyroid stimulating hormone-Gs-ML109 complex
7T9M 3.1 Å, Human Thyrotropin receptor bound by CS-17 Inverse Agonist Fab/Org 274179-0 Antagonist
7XW7 5.5 Å, TSHR-K1-70 complex

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