3G04: TSH receptor

Crystal structure of the TSH receptor in complex with a thyroid-stimulating autoantibody. Determined by X-ray diffraction at 2.55 Å resolution. Released 4 Aug 2009.

Method: X-ray diffraction
Resolution: 2.55 Å
Organism: Homo sapiens
Chains: 3
Atoms: 5,414
Mol. weight: 76.13 kDa
Ligands: NAG, ZN
Released: 4 Aug 2009

Explore 3G04 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3G04 contains 21 α-helices and 72 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 8 helices, 25 β-strands

Element	Residues	Length	Sheet
β-strand	4	1	1
β-strand	5	1	2
β-strand	9-13	4	3
β-strand	19-24	6	2
β-strand	34-38	5	3
β-strand	45-48	4	3
β-strand	49	1	4
β-strand	53	1	4
α-helix	54-55	2
β-strand	62-67	6	2
β-strand	70-75	6	2
α-helix	80-82	3
β-strand	85-92	8	3
β-strand	95B-98	4	3
β-strand	99	1	1
β-strand	102-106	5	3
α-helix	108-110	3
β-strand	111	1	5
α-helix	112-113	2
β-strand	114-118	5	6
α-helix	119-121	3
α-helix	122-126	5
β-strand	130-139	10	6
β-strand	140	1	5
β-strand	145-150	6	7
β-strand	153-155	3	7
β-strand	159-161	3	6
α-helix	162-164	3
β-strand	165-166	2	6
β-strand	172-180	9	6
α-helix	182-187	6
β-strand	191-197	7	7
β-strand	200-206	7	7

Chain B: 10 helices, 24 β-strands

Element	Residues	Length	Sheet
β-strand	3-6	4	8
α-helix	7-9	3
β-strand	10-12	3	9
β-strand	18-25	8	8
α-helix	29-31	3
β-strand	33-39	7	9
β-strand	45-52	8	9
β-strand	56-59	4	9
β-strand	64	1	10
β-strand	67	1	10
β-strand	68-72	5	8
α-helix	73-75	3
β-strand	77-82	6	8
α-helix	84-86	3
β-strand	88-95	8	9
α-helix	96	1
β-strand	100D-103	4	9
β-strand	107-111	5	9
β-strand	117	1	11
α-helix	118-119	2
β-strand	120-124	5	12
β-strand	135-145	11	12
β-strand	146	1	11
β-strand	151-154	4	13
α-helix	155-157	3
β-strand	159	1	13
β-strand	163-165	3	12
α-helix	166-168	3
β-strand	169-170	2	12
β-strand	176-185	10	12
α-helix	186-188	3
β-strand	195-200	6	13
α-helix	201-203	3
β-strand	205-210	6	13

Chain C: 3 helices, 23 β-strands

Element	Residues	Length	Sheet
β-strand	31-34	4	14
β-strand	38-41	4	14
α-helix	49-51	3
β-strand	56-60	5	14
β-strand	66-67	2	15
β-strand	80-84	5	14
β-strand	91-92	2	15
α-helix	93	1
β-strand	97-98	2	16
β-strand	105-111	7	14
β-strand	116-117	2	15
β-strand	122-123	2	16
β-strand	130-136	7	14
α-helix	141-143	3
β-strand	149	1	17
β-strand	153-159	7	14
β-strand	166-167	2	18
β-strand	176	1	17
β-strand	179-183	5	14
β-strand	191-192	2	18
β-strand	201	1	19
β-strand	202-206	5	14
β-strand	215-216	2	18
β-strand	226	1	19
β-strand	230-232	3	14
β-strand	251-253	3	14

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Human thyroid stimulating autoantibody M22 light chain	A	protein	216	Homo sapiens
Human thyroid stimulating autoantibody M22 heavy chain	B	protein	228	Homo sapiens
Thyrotropin receptor	C	protein	239	Homo sapiens	P16473 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>3G04_1 HUMAN THYROID STIMULATING AUTOANTIBODY M22 LIGHT CHAIN (chains A)
LTVLTQPPSVSGAPRQRVTISCSGNSSNIGNNAVNWYQQLPGKAPKLLIYYDDQLPSGVS
DRFSGSRSGTSASLAIRGLQSEDEADYYCTSWDDSLDSQLFGGGTRLTVLGQPKAAPSVT
LFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASS
YLSLTPEQWKSHKSYSCQVTHEGSTVEKTVAPTECS

Sequence of entity 2 (B), FASTA

>3G04_2 HUMAN THYROID STIMULATING AUTOANTIBODY M22 HEAVY CHAIN (chains B)
QVQLVQSGAEVKKPGESLKISCRGSGYRFTSYWINWVRQLPGKGLEWMGRIDPTDSYTNY
SPSFKGHVTVSADKSINTAYLQWSSLKASDTGMYYCARLEPGYSSTWSVNWGQGTLVTVS
SASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQS
SGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTS

Sequence of entity 3 (C), FASTA

>3G04_3 Thyrotropin receptor (chains C)
MGCSSPPCECHQEEDFRVTCKDIQRIPSLPPSTQTLKLIETHLRTIPSHAFSNLPNISRI
YVSIDVTLQQLESHSFYNLSKVTHIEIRNTRNLTYIDPDALKELPLLKFLGIFNTGLKMF
PDLTKVYSTDIFFILEITDNPYMTSIPVNAFQGLCNETLTLKLYNNGFTSVQGYAFNGTK
LDAVYLNKNKYLTVIDKDAFGGVYSGPSLLDVSQTSVTALPSKGLEHLKELIARNTWTL

Ligands and cofactors

ID	Name	Formula	Copies
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	6
ZN	Zinc ion	Zn	5

Primary citation

Crystal structure of the TSH receptor in complex with a thyroid-stimulating autoantibody. Sanders, J., Chirgadze, D.Y., Sanders, P. et al. Thyroid (2007) 17:395-410. DOI 10.1089/thy.2007.0034 · PubMed

Other PDB entries of the same protein (UniProt P16473 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2XWT 1.9 Å, Crystal structure of the tsh receptor in complex with a blocking type tshr autoantibody
7UTZ 2.4 Å, Human thyrotropin analog TR1402 bound to human Thyrotropin receptor in complex with…
7XW6 2.78 Å, TSHR-Gs-M22 antibody-ML109 complex
7T9I 2.9 Å, Native human TSH bound to human Thyrotropin receptor in complex with miniGs399…
7T9N 2.9 Å, M22 Agonist Autoantibody bound to Human Thyrotropin receptor in complex with miniGs399…
7XW5 2.96 Å, TSHR-thyroid stimulating hormone-Gs-ML109 complex
7T9M 3.1 Å, Human Thyrotropin receptor bound by CS-17 Inverse Agonist Fab/Org 274179-0 Antagonist
7XW7 5.5 Å, TSHR-K1-70 complex

Browse structure collections

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