3FIL: Immunoglobulin G-binding protein G

Structural and energetic determinants for hyperstable variants of GB1 obtained from in-vitro evolution. Determined by X-ray diffraction at 0.88 Å resolution. Released 18 Aug 2009.

Method: X-ray diffraction
Resolution: 0.88 Å
Organism: Streptococcus sp. 'group G'
Chains: 2
Atoms: 1,185
Mol. weight: 12.51 kDa
Ligands: CA
Released: 18 Aug 2009

Explore 3FIL in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3FIL contains 3 α-helices and 8 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 2 helices, 4 β-strands

Element	Residues	Length	Sheet
β-strand	2-8	7	1
β-strand	13-19	7	1
α-helix	23-36	14
β-strand	42-46	5	1
α-helix	47-49	3
β-strand	51-55	5	1

Chain B: 1 helix, 4 β-strands

Element	Residues	Length	Sheet
β-strand	2-8	7	1
β-strand	13-19	7	1
α-helix	23-37	15
β-strand	42-46	5	1
β-strand	51-55	5	1

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Immunoglobulin G-binding protein G	A	protein	56	Streptococcus sp. 'group G'	P19909 (AlphaFold model)
Immunoglobulin G-binding protein G	B	protein	56	Streptococcus sp. 'group G'	P19909 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>3FIL_1 Immunoglobulin G-binding protein G (chains A)
MQYKLILNGKTLKGVLTIEAVDAATAEKVFKQYANDLGVDGEWTYDDATKTFTVTE

Sequence of entity 2 (B), FASTA

>3FIL_2 Immunoglobulin G-binding protein G (chains B)
MQYKLILNGKTLKGVLTIEAVDAATAEKVFKQYANDLGVDGEWTYDDATKTFTVTE

Ligands and cofactors

ID	Name	Formula	Copies
CA	Calcium ion	Ca	1

Primary citation

Dimer Formation of a Stabilized Gbeta1 Variant: A Structural and Energetic Analysis. Thoms, S., Max, K.E.A., Wunderlich, M. et al. J Mol Biol (2009) 391:918-932. DOI 10.1016/j.jmb.2009.06.031 · PubMed

Other PDB entries of the same protein (UniProt P19909 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2QMT 1.05 Å, Crystal Polymorphism of Protein GB1 Examined by Solid-state NMR and X-ray Diffraction
9I2I 1.08 Å, X-ray structure of the B1 domain of streptococcal protein G triple mutant T2Q, N8D, and…
6CHE 1.1 Å, Selenomethionine mutant (A34Sem) of protein GB1 examined by X-ray diffraction
6CPZ 1.12 Å, Selenomethionine mutant (I6Sem) of protein GB1 examined by X-ray diffraction
2GI9 1.14 Å, Backbone Conformational Constraints in a Microcrystalline U-15N-Labeled Protein by 3D…
6C9O 1.2 Å, Selenomethionine mutant (V29Sem) of protein GB1 examined by X-ray diffraction
6CTE 1.2 Å, 77Se-NMR probes the protein environment of selenomethionine
6OC7 1.3 Å, HMP42 Fab in complex with Protein G
6NLA 1.34 Å, Crystal structure of de novo designed metal-controlled dimer of B1…
2ON8 1.35 Å, Gbeta1 stabilization by in vitro evolution and computational design
12LO 1.37 Å, X-ray crystal structure of Protein GB1 mutant K13A
6NL6 1.4 Å, Crystal structure of mutant B1 immunoglobulin-binding domain of Streptococcal Protein G…

Browse structure collections

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