3HO4: Hedgehog-interacting protein

Crystal structure of Hedgehog-interacting protein (HHIP). Determined by X-ray diffraction at 3.1 Å resolution. Released 23 Jun 2009.

Method: X-ray diffraction
Resolution: 3.1 Å
Organism: Homo sapiens
Chains: 2
Atoms: 6,868
Mol. weight: 108.83 kDa
Released: 23 Jun 2009

Explore 3HO4 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3HO4 contains 24 α-helices and 86 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 12 helices, 43 β-strands

Element	Residues	Length	Sheet
β-strand	217	1	1
β-strand	218-226	9	2
β-strand	229-233	5	2
β-strand	242-246	5	2
β-strand	250-254	5	2
β-strand	260	1	2
β-strand	266-267	2	2
β-strand	284-289	6	3
α-helix	293-296	4
β-strand	298-304	7	3
β-strand	317-326	10	3
β-strand	334-347	14	3
β-strand	354-359	6	4
α-helix	360	1
α-helix	361-363	3
β-strand	365-369	5	4
α-helix	377-381	5
β-strand	391-395	5	4
β-strand	396	1	5
β-strand	407	1	5
α-helix	408-409	2
β-strand	424-427	4	4
β-strand	432	1	6
β-strand	434	1	7
β-strand	436-438	3	6
β-strand	448-454	7	6
α-helix	460-461	2
β-strand	462-468	7	6
α-helix	472-474	3
α-helix	477-479	3
β-strand	480-483	4	6
β-strand	493-496	4	7
β-strand	509-512	4	7
β-strand	518-523	6	7
α-helix	524	1
β-strand	530-535	6	7
β-strand	537	1	1
β-strand	540	1	8
β-strand	543	1	8
β-strand	544	1	2
β-strand	550-556	7	2
β-strand	562-567	6	2
β-strand	577-583	7	2
β-strand	588	1	9
α-helix	592-594	3
α-helix	607-611	5
β-strand	615-617	3	10
β-strand	623-625	3	10
β-strand	629-630	2	11
β-strand	636-637	2	11
α-helix	642-643	2
β-strand	648-651	4	12
β-strand	654-656	3	12
β-strand	662	1	13
β-strand	668	1	13

Chain B: 12 helices, 43 β-strands

Element	Residues	Length	Sheet
β-strand	217	1	14
β-strand	218-226	9	15
β-strand	229-233	5	15
β-strand	242-246	5	15
β-strand	250-254	5	15
β-strand	260	1	15
β-strand	266-267	2	15
β-strand	284-289	6	16
α-helix	293-296	4
β-strand	298-304	7	16
β-strand	317-326	10	16
β-strand	334-347	14	16
β-strand	354-359	6	17
α-helix	361-363	3
β-strand	365-369	5	17
α-helix	377-381	5
β-strand	391-395	5	17
β-strand	396	1	18
β-strand	407	1	18
α-helix	408-409	2
β-strand	424-427	4	17
β-strand	432	1	19
β-strand	434	1	20
α-helix	435	1
β-strand	436-438	3	19
β-strand	448-454	7	19
α-helix	460-461	2
β-strand	462-468	7	19
α-helix	477-479	3
β-strand	482-483	2	19
β-strand	493-496	4	20
β-strand	509-512	4	20
β-strand	518-521	4	20
β-strand	532-535	4	20
β-strand	537	1	14
β-strand	540	1	21
β-strand	542-543	2	21
β-strand	544	1	15
β-strand	550-556	7	15
β-strand	562-567	6	15
α-helix	569-573	5
β-strand	577-583	7	15
β-strand	588	1	9
α-helix	592-594	3
α-helix	607-611	5
β-strand	617	1	22
β-strand	623	1	22
α-helix	624-626	3
β-strand	629-630	2	23
β-strand	636-637	2	23
α-helix	638	1
β-strand	648-651	4	24
β-strand	654-656	3	24
β-strand	662	1	25
β-strand	668	1	25

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Hedgehog-interacting protein	A, B	protein	481	Homo sapiens	Q96QV1 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>3HO4_1 Hedgehog-interacting protein (chains A, B)
SNYLDQMEEYDKVEEISRKHKHNCFCIQEVVSGLRQPVGALHSGDGSQRLFILEKEGYVK
ILTPEGEIFKEPYLDIHKLVQSGIKGGDERGLLSLAFHPNYKKNGKLYVSYTTNQERWAI
GPHDHILRVVEYTVSRKNPHQVDLRTARVFLEVAELHRKHLGGQLLFGPDGFLYIILGDG
MITLDDMEEMDGLSDFTGSVLRLDVDTDMCNVPYSIPRSNPHFNSTNQPPEVFAHGLHDP
GRCAVDRHPTDININLTILCSDSNGKNRSSARILQIIKGKDYESEPSLLEFKPFSNGPLV
GGFVYRGCQSERLYGSYVFGDRNGNFLTLQQSPVTKQWQEKPLCLGTSGSCRGYFSGHIL
GFGEDELGEVYILSSSKSMTQTHNGKLYKIVDPKRPLMPEECRATVQPAQTLTSECSRLC
RNGYCTPTGKCCCSPGWEGDFCRTAKCEPACRHGGVCVRPNKCLCKKGYLGPQCEHHHHH
H

Primary citation

The structure of SHH in complex with HHIP reveals a recognition role for the Shh pseudo active site in signaling. Bosanac, I., Maun, H.R., Scales, S.J. et al. Nat Struct Mol Biol (2009) 16:691-697. DOI 10.1038/nsmb.1632 · PubMed

Other PDB entries of the same protein (UniProt Q96QV1 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7PGN 2.4 Å, HHP-C in complex with glycosaminoglycan mimic SOS
2WG3 2.6 Å, Crystal structure of the complex between human hedgehog-interacting protein HIP and…
7PGL 2.63 Å, HHIP-N, the N-terminal domain of the Hedgehog-Interacting Protein (HHIP), apo-form
7PGM 2.7 Å, HHIP-C in complex with heparin
7PGK 2.75 Å, HHIP-N, the N-terminal domain of the Hedgehog-Interacting Protein (HHIP), in complex…
2WFT 2.8 Å, Crystal structure of the human HIP ectodomain
3HO3 2.9 Å, Crystal structure of Hedgehog-interacting protein (HHIP)
3HO5 3.01 Å, Crystal structure of Hedgehog-interacting protein (HHIP) and Sonic hedgehog (SHH) complex
2WG4 3.15 Å, Crystal structure of the complex between human hedgehog-interacting protein HIP and…
2WFX 3.2 Å, Crystal structure of the complex between human hedgehog-interacting protein HIP and…

Browse structure collections

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