3NEZ: MRojoA

Name: PDB 3NEZ: mRojoA
Published: 2010-10-13
License: https://creativecommons.org/publicdomain/zero/1.0/
Keywords: fluorescent protein

mRojoA. Determined by X-ray diffraction at 1.7 Å resolution. Released 13 Oct 2010.

Method: X-ray diffraction
Resolution: 1.7 Å
Organism: Discosoma Sp.
Chains: 4
Atoms: 7,612
Mol. weight: 110.81 kDa
Released: 13 Oct 2010

Explore 3NEZ in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3NEZ contains 31 α-helices and 53 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 7 helices, 14 β-strands

Element	Residues	Length	Sheet
β-strand	12-22	11	1
β-strand	25-36	12	1
β-strand	41-50	10	1
α-helix	52	1
α-helix	54	1
α-helix	58-60	3
α-helix	62-64	3
β-strand	74	1	1
α-helix	82-85	4
β-strand	91-99	9	1
β-strand	104-114	11	1
β-strand	117-127	11	1
β-strand	140-143	4	1
α-helix	144-145	2
β-strand	146-153	8	1
β-strand	156-167	12	1
β-strand	172-183	12	1
α-helix	188-190	3
β-strand	192	1	2
β-strand	193-204	12	1
β-strand	210-220	11	1

Chain B: 9 helices, 13 β-strands

Element	Residues	Length	Sheet
β-strand	12-22	11	3
β-strand	25-36	12	3
β-strand	41-50	10	3
α-helix	52	1
α-helix	54	1
α-helix	58-60	3
α-helix	62-64	3
α-helix	70-72	3
β-strand	74	1	3
α-helix	82-85	4
β-strand	91-99	9	3
β-strand	104-114	11	3
β-strand	117-127	11	3
β-strand	143	1	3
α-helix	144-145	2
β-strand	146-153	8	3
β-strand	156-166	11	3
α-helix	168-170	3
β-strand	172-183	12	3
α-helix	188-190	3
β-strand	193-204	12	3
β-strand	210-220	11	3

Chain C: 8 helices, 13 β-strands

Element	Residues	Length	Sheet
β-strand	12-22	11	4
β-strand	25-36	12	4
β-strand	41-50	10	4
α-helix	52	1
α-helix	54	1
α-helix	58-60	3
α-helix	62-64	3
α-helix	70-72	3
β-strand	74	1	4
α-helix	82-85	4
β-strand	91-99	9	4
β-strand	104-114	11	4
β-strand	117-127	11	4
β-strand	140-143	4	4
α-helix	144-145	2
β-strand	146-153	8	4
β-strand	156-167	12	4
β-strand	172-183	12	4
α-helix	188-192	5
β-strand	193-204	12	4
β-strand	210-220	11	4

Chain D: 7 helices, 13 β-strands

Element	Residues	Length	Sheet
β-strand	12-22	11	2
β-strand	25-36	12	2
β-strand	41-50	10	2
α-helix	52	1
α-helix	54	1
α-helix	58-60	3
α-helix	62-64	3
β-strand	74	1	2
α-helix	82-85	4
β-strand	91-99	9	2
β-strand	104-114	11	2
β-strand	117-127	11	2
β-strand	140-143	4	2
α-helix	144-145	2
β-strand	146-153	8	2
β-strand	156-167	12	2
β-strand	172-183	12	2
α-helix	188-190	3
β-strand	193-204	12	2
β-strand	210-220	11	2

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
mRojoA	A, B, C, D	protein	242	Discosoma Sp.	D1MPT3 (AlphaFold model)

Sequence of entity 1 (A, B, C, D), FASTA

>3NEZ_1 mRojoA (chains A, B, C, D)
MGHHHHHHGVSKGEEDNMAIIKEFMRFKTHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLK
VTKGGPLPFAWDILSPQFXSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQ
DSSLQDGEFIYKVKLHGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKLRLKLKD
GGHYDAEVKTTYKAKKPVQLPGAYNANYKLDITSHNEDYTIVEQYERCEGRHSTGGMDEL
YK

Primary citation

Generation of longer emission wavelength red fluorescent proteins using computationally designed libraries. Chica, R.A., Moore, M.M., Allen, B.D. et al. Proc Natl Acad Sci U S A (2010) 107:20257-20262. DOI 10.1073/pnas.1013910107 · PubMed

Other PDB entries of the same protein (UniProt D1MPT3 (AlphaFold model), which also has an AlphaFold model), best resolution first:

3NED 0.95 Å, mRouge
6MZ3 1.09 Å, mCherry pH sensitive mutant - M66T (mCherryTYG)
3LF3 1.15 Å, Crystal Structure of Fast Fluorescent Timer Fast-FT
7SAK 1.15 Å, Crystal Structure of LaM4 Nanobody bound to mCherry
31AL 1.5 Å, Synchrotron structure of non-photoactivated PAmCherry1
3KCS 1.5 Å, Crystal structure of PAmCherry1 in the dark state
9FEL 1.5 Å, LSSmCherry1 - Directionality of Optical Properties of Fluorescent Proteins
6YLM 1.6 Å, mCherry
31JT 1.63 Å, SFX structure of non-photoactivated PAmCherry1
3KCT 1.65 Å, CRYSTAL STRUCTURE OF PAmCherry1 in the photoactivated state
5H89 1.76 Å, Crystal structure of mRojoA mutant - T16V - P63Y - W143G - L163V
3LF4 1.81 Å, Crystal Structure of Fluorescent Timer Precursor Blue102

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