3SG3: GCaMP3-D380Y

Crystal Structure of GCaMP3-D380Y. Determined by X-ray diffraction at 2.1 Å resolution. Released 20 Jun 2012.

Method: X-ray diffraction
Resolution: 2.1 Å
Organisms: Gallus gallus, Aequorea victoria, Rattus norvegicus
Chains: 1
Atoms: 3,470
Mol. weight: 50.91 kDa
Ligands: CA
Released: 20 Jun 2012

Explore 3SG3 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3SG3 contains 18 α-helices and 18 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 18 helices, 18 β-strands

Element	Residues	Length	Sheet
α-helix	40-56	17
β-strand	62-67	6	1
α-helix	68-70	3
β-strand	72-82	11	1
β-strand	83	1	2
β-strand	88-99	12	1
α-helix	106-109	4
β-strand	111-120	10	1
β-strand	129-139	11	1
α-helix	160-165	6
β-strand	168-179	12	1
β-strand	182-193	12	1
α-helix	194-196	3
β-strand	198-205	8	1
α-helix	214-217	4
α-helix	226-228	3
β-strand	230	1	1
α-helix	233-238	6
α-helix	240-243	4
β-strand	249-257	9	1
β-strand	262-272	11	1
β-strand	275-285	11	1
β-strand	298	1	2
α-helix	308-321	14
β-strand	328-329	2	3
α-helix	331-340	10
α-helix	347-355	9
β-strand	365-366	2	3
α-helix	367-379	13
α-helix	381-383	3
α-helix	384-394	11
β-strand	401-402	2	4
α-helix	404-413	10
α-helix	420-430	11
β-strand	438-439	2	4
α-helix	440-446	7

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Myosin light chain kinase, Green fluorescent protein, Calmodulin-1 chimera	A	protein	449	Gallus gallus, Aequorea victoria, Rattus norvegicus	P0DP31 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>3SG3_1 Myosin light chain kinase, Green fluorescent protein, Calmodulin-1 chimera (chains A)
MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNKTGHAVRAIGRLSSL
ENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSVQSKL
SKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDV
NGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLXVQCFSRYPDHMKQHDFF
KSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYN
TRDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADG
DGTIDFPEFLTMMARKMKYTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDE
EVDEMIREADIDGDGQVNYEEFVQMMTAK

Ligands and cofactors

ID	Name	Formula	Copies
CA	Calcium ion	Ca	4

Primary citation

Optimization of a GCaMP calcium indicator for neural activity imaging. Akerboom, J., Chen, T.W., Wardill, T.J. et al. J Neurosci (2012) 32:13819-13840. DOI 10.1523/JNEUROSCI.2601-12.2012 · PubMed

Other PDB entries of the same protein (UniProt P0DP31 (AlphaFold model), which also has an AlphaFold model), best resolution first:

3SG6 1.7 Å, Crystal Structure of Dimeric GCaMP2-LIA(linker 1)
3EVU 1.75 Å, Crystal structure of Calcium bound dimeric GCAMP2
3EK7 1.85 Å, Calcium-saturated GCaMP2 dimer
3SG5 1.9 Å, Crystal Structure of Dimeric GCaMP3-D380Y, QP(linker 1), LP(linker 2)
3SG7 1.9 Å, Crystal Structure of GCaMP3-KF(linker 1)
3EKH 2.0 Å, Calcium-saturated GCaMP2 T116V/K378W mutant monomer
3SG2 2.0 Å, Crystal Structure of GCaMP2-T116V,D381Y
4I2Y 2.2 Å, Crystal Structure of the genetically encoded calcium indicator RGECO1
3SG4 2.4 Å, Crystal Structure of GCaMP3-D380Y, LP(linker 2)
3WLC 2.49 Å, Crystal structure of dimeric GCaMP6m
3EK4 2.65 Å, Calcium-saturated GCaMP2 Monomer
3WLD 2.7 Å, Crystal structure of monomeric GCaMP6m

Browse structure collections

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