3TRV: L-Villin-1

Crystal structure of quasiracemic villin headpiece subdomain containing (F5Phe17) substitution. Determined by X-ray diffraction at 1.0 Å resolution. Released 25 Jan 2012.

Method: X-ray diffraction
Resolution: 1.0 Å
Organism: Gallus gallus
Chains: 2
Atoms: 723
Mol. weight: 8.67 kDa
Ligands: IPA
Released: 25 Jan 2012

Explore 3TRV in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3TRV contains 6 α-helices and 0 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chains A and B: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	3-10	8
α-helix	14-18	5
α-helix	22-30	9

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
L-Villin-1	A	protein	35	Gallus gallus	P02640 (AlphaFold model)
D-Villin-1	B	protein	35	Gallus gallus	P02640 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>3TRV_1 L-Villin-1 (chains A)
LSDEDFKAVFGMTRSAFANLPLWKQQHLKKEKGLF

Sequence of entity 2 (B), FASTA

>3TRV_2 D-Villin-1 (chains B)
LSDEDFKAVFGMTRSAFANLPLWKQQHLKKEKGLF

Ligands and cofactors

ID	Name	Formula	Copies
IPA	Isopropyl alcohol	C3 H8 O	2

Water and common crystallization additives (SO4) are not listed.

Primary citation

Quasiracemic crystallization as a tool to assess the accommodation of noncanonical residues in nativelike protein conformations. Mortenson, D.E., Satyshur, K.A., Guzei, I.A. et al. J Am Chem Soc (2012) 134:2473-2476. DOI 10.1021/ja210045s · PubMed

Other PDB entries of the same protein (UniProt P02640 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1WY3 0.95 Å, Chicken villin subdomain HP-35, K65(NLE), N68H, pH7.0
1YRI 1.0 Å, Chicken villin subdomain HP-35, N68H, pH6.4
2F4K 1.05 Å, Chicken villin subdomain HP-35, K65(NLE), N68H, K70(NLE), PH9
1YRF 1.07 Å, Chicken villin subdomain HP-35, N68H, pH6.7
5I1S 1.12 Å, Villin headpiece subdomain with a Lys30 to APC substitution
5I1N 1.3 Å, Villin headpiece subdomain with a Gln26 to beta-3-homoglutamine substitution
5I1O 1.35 Å, Villin headpiece subdomain with a Gln26 to ACPC substitution
1YU5 1.4 Å, Crystal Structure of the Headpiece Domain of Chicken Villin
2RJY 1.4 Å, Crystal structure of villin headpiece, P21 21 21 space group
5I1P 1.4 Å, Villin headpiece subdomain with a Lys30 to beta-3-homolysine substitution
1YU8 1.45 Å, Crystal Structure of the R37A Mutant of Villin Headpiece
2RJV 1.45 Å, Crystal structure of the H41Y mutant of villin headpiece, P 21 21 21 space group

Browse structure collections

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