Complex between human RanGAP1-SUMO1, UBC9 and the IR1 domain from RanBP2 containing IR2 Motif II. Determined by X-ray diffraction at 2.29 Å resolution. Released 28 Dec 2011.
Explore 3UIP in 3D Show helices and sheets RCSB PDB PDBe
3UIP contains 26 α-helices and 15 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 5-18 | 14 | |
| β-strand | 25-30 | 6 | 1 |
| α-helix | 31 | 1 | |
| β-strand | 36-46 | 11 | 1 |
| α-helix | 47-48 | 2 | |
| β-strand | 57-63 | 7 | 1 |
| α-helix | 72-73 | 2 | |
| β-strand | 74-77 | 4 | 1 |
| β-strand | 86 | 1 | 2 |
| β-strand | 91 | 1 | 1 |
| β-strand | 92 | 1 | 2 |
| β-strand | 94 | 1 | 3 |
| α-helix | 95-97 | 3 | |
| α-helix | 109-121 | 13 | |
| α-helix | 131-139 | 9 | |
| α-helix | 141-154 | 14 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 21-28 | 8 | 4 |
| β-strand | 33-39 | 7 | 4 |
| α-helix | 45-55 | 11 | |
| α-helix | 59-61 | 3 | |
| β-strand | 62-66 | 5 | 4 |
| β-strand | 69-70 | 2 | 4 |
| α-helix | 71-72 | 2 | |
| α-helix | 77-80 | 4 | |
| β-strand | 87-92 | 6 | 4 |
| β-strand | 96 | 1 | 3 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 433-439 | 7 | |
| α-helix | 443-448 | 6 | |
| α-helix | 453-460 | 8 | |
| α-helix | 466-477 | 12 | |
| α-helix | 484-502 | 19 | |
| α-helix | 509-519 | 11 | |
| α-helix | 536-545 | 10 | |
| α-helix | 553-555 | 3 | |
| α-helix | 556-564 | 9 | |
| α-helix | 568-572 | 5 | |
| α-helix | 574-585 | 12 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 2632-2637 | 6 | 4 |
| α-helix | 2642-2650 | 9 | |
| α-helix | 2657-2661 | 5 | |
| α-helix | 2677-2682 | 6 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| SUMO-conjugating enzyme UBC9 | A | protein | 158 | Homo sapiens | P63279 (AlphaFold model) |
| Small ubiquitin-related modifier 1 | B | protein | 80 | Homo sapiens | P63165 (AlphaFold model) |
| Ran GTPase-activating protein 1 | C | protein | 171 | Homo sapiens | P46060 (AlphaFold model) |
| E3 SUMO-protein ligase RanBP2 | D | protein | 67 | Homo sapiens | P49792 |
>3UIP_1 SUMO-conjugating enzyme UBC9 (chains A) MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKL RMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELL NEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS
>3UIP_2 Small ubiquitin-related modifier 1 (chains B) EGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTP KELGMEEEDVIEVYQEQTGG
>3UIP_3 Ran GTPase-activating protein 1 (chains C) SLTGEPAPVLSSPPPADVSTFLAFPSPEKLLRLGPKSSVLIAQQTDTSDPEKVVSAFLKV SSVFKDEATVRMAVQDAVDALMQKAFNSSSFNSNTFLTRLLVHMGLLKSEDKVKAIANLY GPLMALNHMVQQDYFPKALAPLLLAFVTKPNSALESCSFARHSLLQTLYKV
>3UIP_4 E3 SUMO-protein ligase RanBP2 (chains D) SLDVLIVYELTPTVEEKAKADTLKLPPTFFCYKNRPDYVSEEEEDDEDFETAVKKLNGKL YLDGSEK
Determinants of small ubiquitin-like modifier 1 (SUMO1) protein specificity, E3 ligase, and SUMO-RanGAP1 binding activities of nucleoporin RanBP2. Gareau, J.R., Reverter, D., Lima, C.D. J Biol Chem (2012) 287:4740-4751. DOI 10.1074/jbc.M111.321141 · PubMed
Other PDB entries of the same protein (UniProt P63279 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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