3WUS: Vif-Binding Domain of Human APOBEC3F

Crystal Structure of the Vif-Binding Domain of Human APOBEC3F. Determined by X-ray diffraction at 2.54 Å resolution. Released 24 Jun 2015.

Method: X-ray diffraction
Resolution: 2.54 Å
Organism: Homo sapiens
Chains: 2
Atoms: 3,139
Mol. weight: 45.87 kDa
Ligands: ZN
Released: 24 Jun 2015

Explore 3WUS in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3WUS contains 18 α-helices and 20 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 10 β-strands

Element	Residues	Length	Sheet
β-strand	192	1	1
β-strand	195	1	2
α-helix	197-203	7
β-strand	217-225	9	1
β-strand	232-239	8	1
β-strand	241	1	3
β-strand	248	1	3
α-helix	250-261	12
β-strand	268-277	10	1
α-helix	278-280	3
α-helix	281-293	13
β-strand	297-305	9	1
α-helix	312-323	12
β-strand	327-330	4	1
α-helix	333-343	11
β-strand	344	1	2
α-helix	349-351	3
α-helix	353-354	2
α-helix	357-371	15

Chain B: 9 helices, 10 β-strands

Element	Residues	Length	Sheet
β-strand	192	1	4
β-strand	195	1	5
α-helix	197-203	7
β-strand	217-226	10	4
β-strand	232-239	8	4
β-strand	241	1	6
β-strand	248	1	6
α-helix	250-261	12
β-strand	267-277	11	4
α-helix	278-280	3
α-helix	281-292	12
β-strand	297-305	9	4
α-helix	312-323	12
β-strand	327-330	4	4
α-helix	333-343	11
β-strand	344	1	5
α-helix	349-351	3
α-helix	353-354	2
α-helix	357-371	15

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
DNA dC->dU-editing enzyme APOBEC-3F	A, B	protein	190	Homo sapiens	Q8IUX4 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>3WUS_1 DNA dC->dU-editing enzyme APOBEC-3F (chains A, B)
MNPILRNPMEAMYPHIFYFHFKNLRKAYGRNESWLCFTMEVVKHHSPVSWKRGVFRNQVD
PETHCHAERCFLSWFCDDILSPNTNYEVTWYTSWSPCPECAGEVAEFLARHSNVNLTIFT
ARLYYFWDTDYQEGLRSLSQEGASVEIMGYKDFKYCWENFVYNDDEPFKPWKGLKYNFLF
LDSKLQEILE

Ligands and cofactors

ID	Name	Formula	Copies
ZN	Zinc ion	Zn	2

Primary citation

Structural Insights into HIV-1 Vif-APOBEC3F Interaction. Nakashima, M., Ode, H., Kawamura, T. et al. J Virol (2015) 90:1034-1047. DOI 10.1128/JVI.02369-15 · PubMed

Other PDB entries of the same protein (UniProt Q8IUX4 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5HX4 1.92 Å, Zinc-Free APOBEC3F Catalytic Domain Crystal Structure
5ZVB 2.0 Å, APOBEC3F Chimeric Catalytic Domain in Complex with DNA(dT9)
5ZVA 2.3 Å, APOBEC3F Chimeric Catalytic Domain in Complex with DNA(dC9)
5HX5 2.33 Å, APOBEC3F Catalytic Domain Crystal Structure
8VUD 2.6 Å, Crystal structure of APOBEC3F-CD1
4IOU 2.75 Å, Crystal structure of the HIV-1 Vif binding, catalytically active domain of APOBEC3F
4J4J 3.1 Å, Crystal structure of the APOBEC3F Vif binding domain
5W2M 3.7 Å, APOBEC3F Catalytic Domain Complex with a Single-Stranded DNA
6NIL 3.9 Å, cryoEM structure of the truncated HIV-1 Vif/CBFbeta/A3F complex

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