3ZMF: PDB entry 3ZMF

Salmonella enterica SadA 303-358 fused to GCN4 adaptors (SadAK2). Determined by X-ray diffraction at 1.85 Å resolution. Released 20 Feb 2013.

Method: X-ray diffraction
Resolution: 1.85 Å
Organisms: SACCHAROMYCES CEREVISIAE, SALMONELLA ENTERICA SUBSP. ENTERICA SEROVAR TYPHIMURIUM
Chains: 3
Atoms: 2,550
Mol. weight: 37.45 kDa
Released: 20 Feb 2013

Explore 3ZMF in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3ZMF contains 10 α-helices and 12 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 4 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	279-290	12
α-helix	293-316	24
β-strand	319-321	3	1
β-strand	326-328	3	1
β-strand	330-331	2	2
β-strand	334-335	2	2
α-helix	341-345	5
α-helix	356-386	31

Chain B: 3 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	278-316	39
β-strand	319-321	3	3
β-strand	326-328	3	3
β-strand	330-331	2	4
β-strand	334-335	2	4
α-helix	341-345	5
α-helix	356-386	31

Chain C: 3 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	278-316	39
β-strand	319-321	3	5
β-strand	326-328	3	5
β-strand	331	1	6
β-strand	334	1	6
α-helix	341-345	5
α-helix	356-386	31

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
General control protein GCN4, putative inner membrane protein, general control protein GCN4	A, B, C	protein	113	SACCHAROMYCES CEREVISIAE, SALMONELLA ENTERICA SUBSP. ENTERICA SEROVAR TYPHIMURIUM	P03069 (AlphaFold model), Q8ZL64 (AlphaFold model)

Sequence of entity 1 (A, B, C), FASTA

>3ZMF_1 GENERAL CONTROL PROTEIN GCN4, PUTATIVE INNER MEMBRANE PROTEIN, GENERAL CONTROL PROTEIN GCN4 (chains A, B, C)
MKQIEDKIEEILSKIYHIENEIARIKKLITNTEASVAGLAEDALLWDESISAFSASHTGN
ASKITNLAAGTLAADSTDAVNGSQMKQIEDKIEEILSKIYHIENEIARIKKLI

Primary citation

A New Expression System for Protein Crystallization Using Trimeric Coiled-Coil Adaptors. Hernandez Alvarez, B., Hartmann, M.D., Albrecht, R. et al. Protein Eng Des Sel (2008) 21:11. DOI 10.1093/PROTEIN/GZM071 · PubMed

Other PDB entries of the same protein (UniProt P03069 (AlphaFold model), which also has an AlphaFold model), best resolution first:

3AZD 0.98 Å, Crystal structure of tropomyosin N-terminal fragment at 0.98A resolution
2WQ1 1.08 Å, GCN4 leucine zipper mutant with three IxxNTxx motifs coordinating bromide
2WQ0 1.12 Å, GCN4 leucine zipper mutant with three IxxNTxx motifs coordinating chloride
4OWI 1.2 Å, peptide structure
2WQ3 1.22 Å, GCN4 leucine zipper mutant with three IxxNTxx motifs coordinating chloride and nitrate
2HY6 1.25 Å, A seven-helix coiled coil
2WPZ 1.25 Å, GCN4 leucine zipper mutant with two VxxNxxx motifs coordinating chloride
6PSA 1.3 Å, PIE12 D-peptide against HIV entry (in complex with IQN17 Q577R resistance mutant)
2IPZ 1.35 Å, A Parallel Coiled-Coil Tetramer with Offset Helices
2YNY 1.35 Å, Salmonella enterica SadA 255-302 fused to GCN4 adaptors (SadAK1)
5APU 1.35 Å, Sequence IANKEDKAD inserted between GCN4 adaptors - Structure A9b black
2WQ2 1.36 Å, GCN4 leucine zipper mutant with three IxxNTxx motifs coordinating iodide

Browse structure collections

About this viewer

MolViewer shows 3ZMF directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.