4B1V: Phactr1 RPEL-N domain

Structure of the Phactr1 RPEL-N domain bound to G-actin. Determined by X-ray diffraction at 1.75 Å resolution. Released 7 Nov 2012.

Method: X-ray diffraction
Resolution: 1.75 Å
Organisms: ORYCTOLAGUS CUNICULUS, MUS MUSCULUS
Chains: 4
Atoms: 6,803
Mol. weight: 93.9 kDa
Ligands: ATP, LAB, MG
Released: 7 Nov 2012

Explore 4B1V in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4B1V contains 54 α-helices and 38 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 24 helices, 19 β-strands

Element	Residues	Length	Sheet
β-strand	8-12	5	1
β-strand	16-21	6	1
β-strand	29-32	4	1
β-strand	35-36	2	2
β-strand	53-54	2	2
α-helix	56-59	4
β-strand	67-68	2	2
β-strand	71-72	2	3
β-strand	75-76	2	3
α-helix	79-88	10
α-helix	89-94	6
α-helix	98-100	3
β-strand	103-107	5	1
α-helix	113-121	9
α-helix	122-126	5
β-strand	131-136	6	1
α-helix	137-144	8
β-strand	150-155	6	4
β-strand	160-166	7	4
β-strand	169-170	2	4
α-helix	172-174	3
β-strand	176-178	3	4
α-helix	182-193	12
α-helix	194-196	3
α-helix	203-216	14
α-helix	223-232	10
β-strand	238-241	4	5
β-strand	247-250	4	5
α-helix	253-259	7
α-helix	264-267	4
α-helix	274-283	10
α-helix	287-294	8
β-strand	297-300	4	4
α-helix	302-304	3
α-helix	309-320	12
α-helix	325-327	3
β-strand	329-330	2	4
α-helix	335-337	3
α-helix	338-347	10
α-helix	350-352	3
β-strand	357-358	2	1
α-helix	359-365	7
α-helix	369-373	5

Chain B: 24 helices, 19 β-strands

Element	Residues	Length	Sheet
α-helix	6-7	2
β-strand	8-12	5	6
β-strand	16-21	6	6
β-strand	29-32	4	6
β-strand	35-37	3	7
β-strand	53-54	2	7
α-helix	56-59	4
β-strand	66-68	3	7
β-strand	71-72	2	8
β-strand	75-76	2	8
α-helix	79-87	9
α-helix	88-94	7
α-helix	98-100	3
β-strand	103-107	5	6
α-helix	113-125	13
β-strand	131-136	6	6
α-helix	137-144	8
β-strand	150-155	6	9
β-strand	160-166	7	9
β-strand	169-170	2	9
α-helix	172-174	3
β-strand	176-178	3	9
α-helix	182-193	12
α-helix	194-196	3
α-helix	203-216	14
α-helix	223-232	10
β-strand	238-241	4	10
β-strand	247-250	4	10
α-helix	253-259	7
α-helix	264-267	4
α-helix	274-283	10
α-helix	287-294	8
β-strand	297-300	4	9
α-helix	302-305	4
α-helix	309-320	12
α-helix	325-327	3
β-strand	329-330	2	9
α-helix	335-337	3
α-helix	338-347	10
α-helix	350-352	3
β-strand	357-358	2	6
α-helix	359-365	7
α-helix	369-373	5

Chains M and N: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	138-145	8
α-helix	147-149	3
α-helix	150-155	6

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Actin, alpha skeletal muscle	A, B	protein	376	ORYCTOLAGUS CUNICULUS	P68135 (AlphaFold model)
Phosphatase and actin regulator 1	M, N	protein	32	MUS MUSCULUS	Q2M3X8 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>4B1V_1 ACTIN, ALPHA SKELETAL MUSCLE (chains A, B)
CDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQ
SKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKM
TQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLD
LAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKS
YELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVM
SGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITK
QEYDEAGPSIVHRKCF

Sequence of entity 2 (M, N), FASTA

>4B1V_2 PHOSPHATASE AND ACTIN REGULATOR 1 (chains M, N)
FKHTSAALERKISMRQSREELIKRGVLKEIYD

Ligands and cofactors

ID	Name	Formula	Copies
ATP	Adenosine-5'-triphosphate	C10 H16 N5 O13 P3	2
LAB	Latrunculin B	C20 H29 N O5 S	2
MG	Magnesium ion	Mg	2

Water and common crystallization additives (EDO, GOL) are not listed.

Primary citation

Structures of the Phactr1 RPEL domain and RPEL motif complexes with G-actin reveal the molecular basis for actin binding cooperativity. Mouilleron, S., Wiezlak, M., O'Reilly, N. et al. Structure (2012) 20:1960-1970. DOI 10.1016/j.str.2012.08.031 · PubMed

Other PDB entries of the same protein (UniProt P68135 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4B1Y 1.29 Å, Structure of the Phactr1 RPEL-3 bound to G-actin
2FXU 1.35 Å, X-ray Structure of Bistramide A- Actin Complex at 1.35 A resolution.
4K41 1.4 Å, Crystal structure of actin in complex with marine macrolide kabiramide C
1QZ5 1.45 Å, Structure of rabbit actin in complex with kabiramide C
1WUA 1.45 Å, The structure of Aplyronine A-actin complex
2Q0U 1.45 Å, Structure of Pectenotoxin-2 and Latrunculin B Bound to Actin
2V52 1.45 Å, Structure of MAL-RPEL2 complexed to G-actin
3MN5 1.5 Å, Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation
2PBD 1.5 Å, Ternary complex of profilin-actin with the poly-PRO-GAB domain of VASP*
5ZZA 1.53 Å, OdinProfilin/Rabbit Actin Complex
1J6Z 1.54 Å, Uncomplexed actin
1QZ6 1.6 Å, Structure of rabbit actin in complex with jaspisamide A

Browse structure collections

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