4FZ0: Acid-sensing ion channel

Crystal structure of acid-sensing ion channel in complex with psalmotoxin 1 at low pH. Determined by X-ray diffraction at 2.8 Å resolution. Released 1 Aug 2012.

Method: X-ray diffraction
Resolution: 2.8 Å
Organisms: Gallus gallus, Psalmopoeus cambridgei
Chains: 6
Atoms: 10,557
Mol. weight: 169.5 kDa
Ligands: NAG
Released: 1 Aug 2012

Explore 4FZ0 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4FZ0 contains 73 α-helices and 70 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 25 helices, 19 β-strands

Element	Residues	Length	Sheet
α-helix	51-57	7
α-helix	59-69	11
β-strand	74-81	8	1
β-strand	86-87	2	2
β-strand	90-95	6	3
α-helix	101-103	3
α-helix	106-112	7
α-helix	133-142	10
α-helix	155-162	8
α-helix	164-165	2
α-helix	166-169	4
β-strand	170-175	6	1
β-strand	178-179	2	1
α-helix	182-184	3
β-strand	185-190	6	3
β-strand	193-198	6	3
α-helix	205-208	4
β-strand	209-210	2	2
α-helix	215-217	3
β-strand	219-224	6	1
α-helix	227-229	3
α-helix	231-232	2
β-strand	244	1	4
β-strand	246-251	6	3
α-helix	259-262	4
β-strand	264-266	3	3
β-strand	270-282	13	1
β-strand	292	1	5
α-helix	306-322	17
β-strand	325	1	6
α-helix	334	1
β-strand	335	1	6
α-helix	336-337	2
α-helix	338-340	3
α-helix	341-345	5
α-helix	346-350	5
α-helix	351-355	5
α-helix	363-364	2
β-strand	365	1	5
β-strand	367-379	13	1
α-helix	383-393	11
α-helix	397-403	7
β-strand	404-423	20	1
α-helix	429-452	24

Chain B: 24 helices, 17 β-strands

Element	Residues	Length	Sheet
α-helix	49-68	20
β-strand	74-81	8	4
β-strand	86-87	2	7
β-strand	90-95	6	8
α-helix	101-103	3
α-helix	106-112	7
α-helix	133-142	10
α-helix	155-162	8
α-helix	164-165	2
α-helix	166-169	4
β-strand	170-175	6	4
β-strand	178-179	2	4
α-helix	182-184	3
β-strand	185-190	6	8
β-strand	193-198	6	8
α-helix	205-208	4
β-strand	209-210	2	7
α-helix	215-217	3
β-strand	219-224	6	4
α-helix	227-229	3
α-helix	231-232	2
β-strand	244	1	9
β-strand	246-251	6	8
α-helix	259-262	4
β-strand	264-266	3	8
β-strand	270-282	13	4
α-helix	306-322	17
β-strand	325	1	10
α-helix	334	1
β-strand	335	1	10
α-helix	336-337	2
α-helix	338-340	3
α-helix	341-345	5
α-helix	346-350	5
α-helix	351-355	5
α-helix	363-365	3
β-strand	367-379	13	4
α-helix	383-393	11
α-helix	397-403	7
β-strand	404-423	20	4
α-helix	427-448	22

Chain C: 24 helices, 19 β-strands

Element	Residues	Length	Sheet
α-helix	45-69	25
β-strand	74-81	8	9
β-strand	86-87	2	11
β-strand	90-95	6	12
α-helix	101-103	3
α-helix	106-112	7
α-helix	133-142	10
α-helix	150-152	3
α-helix	155-162	8
α-helix	166-169	4
β-strand	170-175	6	9
β-strand	178-179	2	9
α-helix	182-184	3
β-strand	185-190	6	12
β-strand	193-198	6	12
α-helix	205-208	4
β-strand	209-210	2	11
α-helix	215-217	3
β-strand	219-224	6	9
α-helix	227-229	3
α-helix	231-232	2
β-strand	244	1	1
β-strand	246-251	6	12
α-helix	259-262	4
β-strand	264-266	3	12
β-strand	270-282	13	9
β-strand	292	1	13
α-helix	306-322	17
β-strand	325	1	14
α-helix	334	1
β-strand	335	1	14
α-helix	336-337	2
α-helix	338-340	3
α-helix	341-345	5
α-helix	346-350	5
α-helix	351-355	5
α-helix	363-364	2
β-strand	365	1	13
β-strand	367-379	13	9
α-helix	383-393	11
α-helix	397-403	7
β-strand	404-423	20	9
α-helix	427-448	22

Chains M, N and O: 0 helices, 5 β-strands

Element	Residues	Length	Sheet
β-strand	4	1	15
β-strand	9	1	16
β-strand	17	1	15
β-strand	21-24	4	16
β-strand	32-35	4	16

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Acid-sensing ion channel 1	A, B, C	protein	450	Gallus gallus	Q1XA76 (AlphaFold model)
Pi-theraphotoxin-Pc1a	M, N, O	protein	40	Psalmopoeus cambridgei	P60514 (AlphaFold model)

Sequence of entity 1 (A, B, C), FASTA

>4FZ0_1 Acid-sensing ion channel 1 (chains A, B, C)
GQPVSIQAFASSSTLHGISHIFSYERLSLKRVVWALCFMGSLALLALVCTNRIQYYFLYP
HVTKLDEVAATRLTFPAVTFCNLNEFRFSRVTKNDLYHAGELLALLNNRYEIPDTQTADE
KQLEILQDKANFRNFKPKPFNMLEFYDRAGHDIREMLLSCFFRGEQCSPEDFKVVFTRYG
KCYTFNAGQDGKPRLITMKGGTGNGLEIMLDIQQDEYLPVWGETDETSFEAGIKVQIHSQ
DEPPLIDQLGFGVAPGFQTFVSCQEQRLIYLPPPWGDCKATTGDSEFYDTYSITACRIDC
ETRYLVENCNCRMVHMPGDAPYCTPEQYKECADPALDFLVEKDNEYCVCEMPCNVTRYGK
ELSMVKIPSKASAKYLAKKYNKSEQYIGENILVLDIFFEALNYETIEQKKAYEVAGLLGD
IGGQMGLFIGASILTVLELFDYAYEVIKHR

Sequence of entity 2 (M, N, O), FASTA

>4FZ0_2 Pi-theraphotoxin-Pc1a (chains M, N, O)
EDCIPKWKGCVNRHGDCCEGLECWKRRRSFEVCVPKTPKT

Ligands and cofactors

ID	Name	Formula	Copies
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	5

Water and common crystallization additives (GOL, CL) are not listed.

Primary citation

Structural plasticity and dynamic selectivity of acid-sensing ion channel-spider toxin complexes. Baconguis, I., Gouaux, E. Nature (2012) 489:400-405. DOI 10.1038/nature11375 · PubMed

Other PDB entries of the same protein (UniProt Q1XA76 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2QTS 1.9 Å, Structure of an acid-sensing ion channel 1 at 1.9 A resolution and low pH
4NTW 2.07 Å, Structure of acid-sensing ion channel in complex with snake toxin
4NTX 2.27 Å, Structure of acid-sensing ion channel in complex with snake toxin and amiloride
3S3W 2.6 Å, Structure of chicken acid-sensing ion channel 1 at 2.6 a resolution and ph 7.5
4NTY 2.65 Å, Cesium sites in the crystal structure of acid-sensing ion channel in complex with snake…
6VTK 2.82 Å, Structure of an acid-sensing ion channel solubilized by styrene maleic acid and in a…
5WKU 2.95 Å, Structure of an acid sensing ion channel in a resting state with barium
3S3X 2.99 Å, Structure of chicken acid-sensing ion channel 1 AT 3.0 A resolution in complex with…
3IJ4 3.0 Å, Cesium sites in the crystal structure of a functional acid sensing ion channel in the…
4NYK 3.0 Å, Structure of a membrane protein
6X9H 3.01 Å, Molecular mechanism and structural basis of small-molecule modulation of acid-sensing…
5WKV 3.2 Å, Structure of an acid sensing ion channel in a resting state with calcium

Browse structure collections

About this viewer

MolViewer shows 4FZ0 directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.