4I36: 6-phosphofructokinase

Crystal Structure of the Bacillus stearothermophilus Phosphofructokinase Mutant D12A. Determined by X-ray diffraction at 2.3 Å resolution. Released 31 Jul 2013.

Method: X-ray diffraction
Resolution: 2.3 Å
Organism: Geobacillus stearothermophilus
Chains: 4
Atoms: 9,846
Mol. weight: 136.5 kDa
Released: 31 Jul 2013

Explore 4I36 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4I36 contains 67 α-helices and 57 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 16 helices, 14 β-strands

Element	Residues	Length	Sheet
β-strand	3-9	7	1
α-helix	16-29	14
β-strand	33-37	5	1
α-helix	40-45	6
β-strand	49-51	3	1
α-helix	52-53	2
α-helix	54-57	4
β-strand	70	1	2
α-helix	74-77	4
α-helix	79-91	13
β-strand	97-101	5	1
α-helix	103-114	12
β-strand	119-123	5	1
β-strand	124	1	3
β-strand	137	1	3
α-helix	139-154	16
β-strand	161-168	8	4
α-helix	175-183	9
β-strand	188-190	3	4
α-helix	198-210	13
β-strand	216-221	6	4
α-helix	227-238	12
β-strand	241-246	6	4
α-helix	248-252	5
α-helix	255-257	3
α-helix	258-276	19
β-strand	282-287	6	1
β-strand	290-295	6	1
α-helix	296-300	5
α-helix	308-317	10

Chain B: 16 helices, 13 β-strands

Element	Residues	Length	Sheet
β-strand	3-9	7	5
α-helix	16-29	14
β-strand	33-37	5	5
α-helix	40-46	7
β-strand	49-51	3	5
α-helix	54-56	3
α-helix	75-77	3
α-helix	79-91	13
β-strand	96-101	6	5
α-helix	103-114	12
β-strand	119-123	5	5
β-strand	124-125	2	6
β-strand	137-138	2	6
α-helix	139-155	17
β-strand	163-168	6	7
α-helix	175-184	10
β-strand	188-190	3	7
α-helix	198-210	13
β-strand	216-221	6	7
α-helix	227-238	12
β-strand	242-246	5	7
α-helix	248-252	5
α-helix	255-257	3
α-helix	258-276	19
β-strand	282-287	6	5
β-strand	290-295	6	5
α-helix	296-299	4
α-helix	303-304	2
α-helix	308-317	10

Chain C: 17 helices, 15 β-strands

Element	Residues	Length	Sheet
β-strand	3-9	7	8
α-helix	16-29	14
β-strand	33-37	5	8
α-helix	40-45	6
β-strand	49-51	3	8
α-helix	52-53	2
α-helix	54-56	3
α-helix	75-77	3
α-helix	79-92	14
β-strand	97-101	5	8
α-helix	103-114	12
β-strand	119-123	5	8
β-strand	124-125	2	9
β-strand	137-138	2	9
α-helix	139-154	16
β-strand	158	1	10
β-strand	161	1	10
β-strand	163-168	6	7
α-helix	175-183	9
β-strand	188-190	3	7
α-helix	198-210	13
β-strand	216-221	6	7
α-helix	227-238	12
β-strand	242-246	5	7
α-helix	248-252	5
α-helix	255-257	3
α-helix	258-276	19
β-strand	282-287	6	8
β-strand	290-295	6	8
α-helix	296-301	6
α-helix	303-304	2
α-helix	308-317	10

Chain D: 18 helices, 15 β-strands

Element	Residues	Length	Sheet
β-strand	3-9	7	11
α-helix	16-29	14
β-strand	33-37	5	11
α-helix	38	1
α-helix	40-46	7
β-strand	49-51	3	11
α-helix	52-53	2
α-helix	54-57	4
α-helix	74-77	4
α-helix	79-91	13
β-strand	96-101	6	11
α-helix	103-114	12
β-strand	119-123	5	11
β-strand	124-125	2	12
β-strand	137-138	2	12
α-helix	139-153	15
β-strand	158	1	2
β-strand	161	1	2
β-strand	163-168	6	4
α-helix	175-184	10
β-strand	188-190	3	4
α-helix	198-210	13
β-strand	216-221	6	4
α-helix	227-238	12
β-strand	242-246	5	4
α-helix	248-252	5
α-helix	255-257	3
α-helix	258-276	19
β-strand	282-287	6	11
β-strand	290-295	6	11
α-helix	296-300	5
α-helix	303-304	2
α-helix	308-318	11

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
6-phosphofructokinase	A, B, C, D	protein	319	Geobacillus stearothermophilus	P00512 (AlphaFold model)

Sequence of entity 1 (A, B, C, D), FASTA

>4I36_1 6-phosphofructokinase (chains A, B, C, D)
MKRIGVLTSGGASPGMNAAIRSVVRKAIYHGVEVYGVYHGYAGLIAGNIKKLEVGDVGDI
IHRGGTILYTARCPEFKTEEGQKKGIEQLKKHGIEGLVVIGGDGSYQGAKKLTEHGFPCV
GVPGTIDNDIPGTDFTIGFDTALNTVIDAIDKIRDTATSHERTYVIEVMGRHAGDIALWS
GLAGGAETILIPEADYDMNDVIARLKRGHERGKKHSIIIVAEGVGSGVDFGRQIQEATGF
ETRVTVLGHVQRGGSPTAFDRVLASRLGARAVELLLEGKGGRCVGIQNNQLVDHDIAEAL
ANKHTIDQRMYALSKELSI

Primary citation

Redefining the Role of the Quaternary Shift in Bacillus stearothermophilus Phosphofructokinase. Mosser, R., Reddy, M.C., Bruning, J.B. et al. Biochemistry (2013) 52:5421-5429. DOI 10.1021/bi4002503 · PubMed

Other PDB entries of the same protein (UniProt P00512 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4I7E 2.0 Å, Crystal Structure of the Bacillus stearothermophilus Phosphofructokinase Mutant D12A in…
3PFK 2.4 Å, Phosphofructokinase. Structure and control
4PFK 2.4 Å, Phosphofructokinase. Structure and control
4I4I 2.49 Å, Crystal Structure of Bacillus stearothermophilus Phosphofructokinase mutant T156A bound…
6PFK 2.6 Å, Phosphofructokinase, inhibited T-state
3U39 2.79 Å, Crystal Structure of the apo Bacillus Stearothermophilus phosphofructokinase
1MTO 3.2 Å, Crystal structure of a Phosphofructokinase mutant from Bacillus stearothermophilus bound…

Browse structure collections

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