4LTE: Cysteine-free Human Insulin Degrading Enzyme

Structure of Cysteine-free Human Insulin Degrading Enzyme in Complex with Macrocyclic Inhibitor. Determined by X-ray diffraction at 2.71 Å resolution. Released 21 May 2014.

Method

X-ray diffraction

Resolution

2.71 Å

Organisms

Homo sapiens, synthetic construct

Chains

4

Atoms

16,216

Mol. weight

228.6 kDa

Ligands

FUM, LYN, ZN

Released

21 May 2014

Explore 4LTE in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4LTE contains 119 α-helices and 68 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 60 helices, 34 β-strands

Molecules and chains

>4LTE_1 Insulin-degrading enzyme (chains A, B)
MNNPAIKRIGNHITKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPP
NIAGLSHFLQHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGA
LDRFAQFFLSPLFDESAKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTG
NKYTLETRPNQEGIDVRQELLKFHSAYYSSNLMAVVVLGRESLDDLTNLVVKLFSEVENK
NVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHE
GPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKL
RAEGPQEWVFQELKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRP
DLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGK
FKLPTKNEFIPTNFEILPLEKEATPYPALIKDTAMSKLWFKQDDKFFLPKANLNFEFFSP
FAYVDPLHSNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPIL
LKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELK
EALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPS
QLVRYREVQLPDRGWFVYQQRNEVHNNSGIEIYYQTDMQSTSENMFLELFAQIISEPAFN
TLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEA
FQKHIQALAIRRLDKPKKLSAESAKYWGEIISQQYNFDRDNTEVAYLKTLTKEDIIKFYK
EMLAVDAPRRHKVSVHVLAREMDSNPVVGEFPAQNDINLSQAPALPQPEVIQNMTEFKRG
LPLFPLVKPHINFMAAKL

>4LTE_2 Macrocyclic Inhibitor (chains M, N)
QFX

Ligands and cofactors

Water and common crystallization additives (EPE) are not listed.

Primary citation

Anti-diabetic activity of insulin-degrading enzyme inhibitors mediated by multiple hormones. Maianti, J.P., McFedries, A., Foda, Z.H. et al. Nature (2014) 511:94-98. DOI 10.1038/nature13297 · PubMed

Related structures

Other PDB entries of the same protein (UniProt P14735 (AlphaFold model), which also has an AlphaFold model), best resolution first:

• 3CWW 1.96 Å, Crystal Structure of IDE-bradykinin complex
• 2G47 2.1 Å, Crystal structure of human insulin-degrading enzyme in complex with amyloid-beta (1-40)
• 2G56 2.2 Å, crystal structure of human insulin-degrading enzyme in complex with insulin B chain
• 4PES 2.21 Å, Crystal structure of insulin degrading enzyme complexed with inhibitor tert-butyl…
• 4PFC 2.21 Å, Crystal structure of insulin degrading enzyme complexed with inhibitor
• 2G54 2.25 Å, Crystal structure of Zn-bound human insulin-degrading enzyme in complex with insulin B…
• 3E4Z 2.28 Å, Crystal structure of human insulin degrading enzyme in complex with insulin-like growth…
• 3E50 2.3 Å, Crystal structure of human insulin degrading enzyme in complex with transforming growth…
• 4PF7 2.33 Å, Crystal structure of insulin degrading enzyme complexed with inhibitor
• 3OFI 2.35 Å, Crystal structure of human insulin-degrading enzyme in complex with ubiquitin
• 2G49 2.5 Å, Crystal structure of human insulin-degrading enzyme in complex with glucagon
• 4PF9 2.5 Å, Crystal structure of insulin degrading enzyme complexed with inhibitor

Browse structure collections

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