Structure of active human M2 muscarinic acetylcholine receptor bound to the agonist iperoxo and allosteric modulator LY2119620. Determined by X-ray diffraction at 3.7 Å resolution. Released 27 Nov 2013.
Explore 4MQT in 3D Show helices and sheets RCSB PDB PDBe
4MQT contains 20 α-helices and 12 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 22-50 | 29 | |
| α-helix | 52-54 | 3 | |
| α-helix | 58-71 | 14 | |
| α-helix | 72-76 | 5 | |
| α-helix | 77-86 | 10 | |
| α-helix | 93-126 | 34 | |
| α-helix | 131-134 | 4 | |
| α-helix | 137-160 | 24 | |
| α-helix | 162-166 | 5 | |
| α-helix | 179-182 | 4 | |
| α-helix | 184-191 | 8 | |
| α-helix | 192-196 | 5 | |
| α-helix | 197-213 | 17 | |
| α-helix | 380-410 | 31 | |
| α-helix | 420-430 | 11 | |
| α-helix | 432-436 | 5 | |
| α-helix | 437-441 | 5 | |
| α-helix | 445-455 | 11 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 4-6 | 3 | 1 |
| β-strand | 11-12 | 2 | 2 |
| β-strand | 18-24 | 7 | 1 |
| α-helix | 32-34 | 3 | |
| β-strand | 35-42 | 8 | 3 |
| β-strand | 49-55 | 7 | 3 |
| β-strand | 60-63 | 4 | 3 |
| β-strand | 71-76 | 6 | 1 |
| β-strand | 81-86 | 6 | 1 |
| α-helix | 91-93 | 3 | |
| β-strand | 95-103 | 9 | 3 |
| β-strand | 107-111 | 5 | 3 |
| β-strand | 115-117 | 3 | 3 |
| β-strand | 118-119 | 2 | 2 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Muscarinic acetylcholine receptor M2 | A | protein | 351 | Homo sapiens | P08172 (AlphaFold model) |
| Nanobody 9-8 | B | protein | 125 | Lama glama |
>4MQT_1 Muscarinic acetylcholine receptor M2 (chains A) DYKDDDENLYFQGLEMDDSTDSSDNSLALTSPYLEKTFEVVFIVLVAGSLSLVTIIGNIL VMVSIKVNRHLQTVNNYFLFSLACADLIIGVFSMNLYTLYTVIGYWPLGPVVCDLWLALD YVVSNASVMNLLIISFDRYFCVTKPLTYPVKRTTKMAGMMIAAAWVLSFILWAPAILFWQ FIVGVRTVEDGECYIQFFSNAAVTFGTAIAAFYLPVIIMTVLYWHISRASKSRIKKDKKE PVANQDPVSTRKKPPPSREKKVTRTILAILLAFIITWAPYNVMVLINTFCAPCIPNTVWT IGYWLCYINSTINPACYALCNATFKKTFKHLLMCHYKNIGATRHHHHHHHH
>4MQT_2 Nanobody 9-8 (chains B) GPGSQVQLQESGGGLVQAGDSLRLSCAASGFDFDNFDDYAIGWFRQAPGQEREGVSCIDP SDGSTIYADSAKGRFTISSDNAENTVYLQMNSLKPEDTAVYVCSAWTLFHSDEYWGQGTQ VTVSS
| ID | Name | Formula | Copies |
|---|---|---|---|
| IXO | 4-(4,5-dihydro-1,2-oxazol-3-yloxy)-N,N,N-trimethylbut-2-yn-1-aminium | C10 H17 N2 O2 | 1 |
| 2CU | 3-amino-5-chloro-N-cyclopropyl-4-methyl-6-[2-(4-methylpiperazin-1-yl)-2-oxoetho… | C19 H24 Cl N5 O3 S | 1 |
Activation and allosteric modulation of a muscarinic acetylcholine receptor. Kruse, A.C., Ring, A.M., Manglik, A. et al. Nature (2013) 504:101-106. DOI 10.1038/nature12735 · PubMed
Other PDB entries of the same protein (UniProt P08172 (AlphaFold model), which also has an AlphaFold model), best resolution first:
MolViewer shows 4MQT directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.