4PJJ: Unconventional myosin-VI

MYOSIN VI (MD-INSERT2-CAM, DELTA-INSERT1) post-rigor state - long soaking with PO4. Determined by X-ray diffraction at 2.4 Å resolution. Released 29 Apr 2015.

Method: X-ray diffraction
Resolution: 2.4 Å
Organisms: Sus scrofa, Drosophila melanogaster
Chains: 2
Atoms: 7,479
Mol. weight: 107.62 kDa
Ligands: MG, PO4, CA, ADP
Released: 29 Apr 2015

Explore 4PJJ in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4PJJ contains 47 α-helices and 33 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 39 helices, 29 β-strands

Element	Residues	Length	Sheet
β-strand	7-11	5	1
β-strand	15-24	10	1
β-strand	28-33	6	1
β-strand	41-44	4	1
α-helix	45-47	3
β-strand	49-50	2	1
β-strand	61	1	2
α-helix	62-64	3
α-helix	70-82	13
β-strand	87-90	4	2
β-strand	93-97	5	2
α-helix	109-115	7
α-helix	118-119	2
α-helix	127-141	15
β-strand	145-150	6	2
α-helix	157-172	16
α-helix	177-193	17
β-strand	194-195	2	3
β-strand	203-204	2	3
β-strand	207-214	8	2
β-strand	220-228	9	2
α-helix	233-235	3
β-strand	245	1	3
α-helix	246-254	9
α-helix	257-262	6
α-helix	268-270	3
α-helix	313-327	15
α-helix	331-347	17
β-strand	352-353	2	4
β-strand	363-364	2	4
α-helix	366-368	3
α-helix	369-379	11
α-helix	383-389	7
β-strand	392	1	5
β-strand	410	1	5
α-helix	413-440	28
β-strand	450-456	7	2
β-strand	466	1	6
α-helix	468-498	31
α-helix	512-519	8
α-helix	525-533	9
α-helix	540-550	11
β-strand	557-558	2	6
α-helix	560-562	3
α-helix	566-569	4
α-helix	573-575	3
β-strand	576-581	6	6
β-strand	584-589	6	6
α-helix	593-596	4
β-strand	598	1	7
α-helix	600-602	3
α-helix	603-610	8
α-helix	615-620	6
β-strand	642	1	7
α-helix	643-659	17
β-strand	662-669	8	2
α-helix	682-691	10
α-helix	694-702	9
β-strand	707-710	4	8
α-helix	711-718	8
α-helix	719-721	3
α-helix	724-727	4
α-helix	731-742	12
β-strand	749-751	3	8
β-strand	755-758	4	8
α-helix	762-771	10
α-helix	774-783	10
α-helix	785-813	29

Chain B: 8 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	6-19	14
β-strand	27	1	9
α-helix	29-38	10
α-helix	45-53	9
β-strand	63	1	9
α-helix	65-72	8
α-helix	78-92	15
β-strand	99-100	2	10
α-helix	102-111	10
α-helix	118-128	11
β-strand	136-137	2	10
α-helix	138-145	8

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Unconventional myosin-VI	A	protein	788	Sus scrofa	F1RQI7 (AlphaFold model)
Calmodulin	B	protein	149	Drosophila melanogaster	P62152 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>4PJJ_1 Unconventional myosin-VI (chains A)
EDGKPVWAPHPTDGFQVGNIVDIGPDSLTIEPLNQKGKTFLALINQVFPAEEDSKKDVED
NCSLMYLNEATLLHNIKVRYSKDRIYTYVANILIAVNPYFDIPKIYSSETIKSYQGKSLG
TMPPHVFAIADKAFRDMKVLKLSQSIIVSGESGAGKTENTKFVLRYLTESYGTGQDIDDR
IVEANPLLEAFGNAKTVRNNNSSRFGKFVEIHFNEKSSVVGGFVSHYLLEKSRICVQGKE
ERNYHIFYRLCAGASEDIRERLHLSSPDNFRYLNRGGSLKDPLLDDHGDFIRMCTAMKKI
GLDDEEKLDLFRVVAGVLHLGNIDFEEAGSTSGGCNLKNKSTQALEYCAELLGLDQDDLR
VSLTTMVMLTTAGGAKGTVIKVPLKVEQANNARDALAKTVYSHLFDHVVNRVNQCFPFET
SSYFIGVLDIAGFEYFEHNSFEQFCINYCNEKLQQFFNERILKEEQELYQKEGLGVNEVH
YVDNQDCIDLIEARLVGILDILDEENRLPQPSDQHFTSAVHQKHKDHFRLSIPRKSKLAI
HRNIRDDEGFIIRHFAGAVCYETTQFVEKNNDALHMSLESLICESRDKFIRELFESSTNN
NKDTKQKAGKLSFISVGNKFKTQLNLLLDKLRSTGASFIRCIKPNLKMTSHHFEGAQILS
QLQCSGMVSVLDLMQGGFPSRASFHELYNMYKKYMPDKLARLDPRLFCKALFKALGLNEI
DYKFGLTKVFFRPGKFAEFDQIMKSDPDHLAELVKRVNHWLICSRWKKVQWCSLSVIKLK
NKIKYRAE

Sequence of entity 2 (B), FASTA

>4PJJ_2 Calmodulin (chains B)
MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADG
NGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGEKLTDE
EVDEMIRESDIDGDGQVNYEEFVTMMTSK

Ligands and cofactors

ID	Name	Formula	Copies
MG	Magnesium ion	Mg	1
PO4	Phosphate ion	O4 P	2
CA	Calcium ion	Ca	2
ADP	Adenosine-5'-diphosphate	C10 H15 N5 O10 P2	1

Water and common crystallization additives (GOL) are not listed.

Primary citation

How actin initiates the motor activity of Myosin. Llinas, P., Isabet, T., Song, L. et al. Dev Cell (2015) 33:401-412. DOI 10.1016/j.devcel.2015.03.025 · PubMed

Other PDB entries of the same protein (UniProt F1RQI7 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4PFO 1.75 Å, Myosin VI motor domain in the Pi release state, space group P212121
4PJN 2.0 Å, Myosin VI motor domain in the Pi release state, space group P212121 - shortly soaked…
4PJM 2.05 Å, Myosin VI motor domain in the Pi release state, space group P212121 - soaked with PO4 -…
4PJL 2.1 Å, Myosin VI motor domain A458E mutant in the Pi release state, space group P212121 -
4E7S 2.25 Å, Myosin VI D23R I24R R569E (MD) pre-powerstroke state
4E7Z 2.3 Å, Myosin VI (MD) pre-powerstroke state, P21 crystal form
4PFP 2.32 Å, Myosin VI motor domain in the Pi release state (with Pi) space group P21
4PK4 2.78 Å, Myosin VI motor domain in the PPS state - from a Pi release state crystal, space group…

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