4UMO: Kv7.1 proximal C-terminal Domain

Crystal Structure of the Kv7.1 proximal C-terminal Domain in Complex with Calmodulin. Determined by X-ray diffraction at 3.0 Å resolution. Released 5 Nov 2014.

Method: X-ray diffraction
Resolution: 3.0 Å
Organism: HOMO SAPIENS
Chains: 4
Atoms: 3,518
Mol. weight: 60.96 kDa
Ligands: SCN, CA
Released: 5 Nov 2014

Explore 4UMO in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4UMO contains 25 α-helices and 10 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	357-365	9
α-helix	367-383	17
α-helix	390-392	3
α-helix	396-533	33

Chain B: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	353-365	13
α-helix	367-383	17
α-helix	390-392	3
α-helix	396-529	29

Chain C: 8 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	6-19	14
β-strand	26-27	2	1
α-helix	29-38	10
α-helix	45-55	11
β-strand	63-64	2	1
α-helix	65-72	8
α-helix	82-90	9
β-strand	99-101	3	2
α-helix	102-111	10
α-helix	118-128	11
β-strand	132	1	2
β-strand	135-137	3	2
α-helix	138-145	8

Chain D: 9 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	6-19	14
β-strand	26-27	2	3
α-helix	29-38	10
α-helix	45-55	11
β-strand	63-64	2	3
α-helix	65-73	9
α-helix	82-88	7
α-helix	90-92	3
β-strand	99-101	3	4
α-helix	102-111	10
α-helix	118-128	11
β-strand	132	1	4
β-strand	135-137	3	4
α-helix	138-145	8

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Potassium voltage-gated channel subfamily kqt member 1	A, B	protein	112	HOMO SAPIENS	P51787 (AlphaFold model)
Calmodulin	C, D	protein	149	HOMO SAPIENS	P0DP23 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>4UMO_1 POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY KQT MEMBER 1 (chains A, B)
MGSHHHHHHHHGSDYDDIPTTENLYFQGSALKVQQKQRQKHFNRQIPAAASLIQTAWRCY
AAENPDSSTWKIYIEFSQLREHHRATIKVIRRMQYFVAKKKFQQARKPYDVR

Sequence of entity 2 (C, D), FASTA

>4UMO_2 CALMODULIN (chains C, D)
MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADG
NGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDE
EVDEMIREADIDGDGQVNYEEFVQMMTAK

Ligands and cofactors

ID	Name	Formula	Copies
SCN	Thiocyanate ion	C N S	1
CA	Calcium ion	Ca	4

Water and common crystallization additives (K) are not listed.

Primary citation

Structural Basis of a Kv7.1 Potassium Channel Gating Module: Studies of the Intracellular C-Terminal Domain in Complex with Calmodulin. Sachyani, D., Dvir, M., Strulovich, R. et al. Structure (2014) 22:1582. DOI 10.1016/J.STR.2014.07.016 · PubMed

Other PDB entries of the same protein (UniProt P51787 (AlphaFold model), which also has an AlphaFold model), best resolution first:

3HFE 1.7 Å, A trimeric form of the Kv7.1 A domain Tail
3BJ4 2.0 Å, The KCNQ1 (Kv7.1) C-terminus, a multi-tiered scaffold for subunit assembly and protein…
7VVH 2.3 Å, Crystal Structure of the Kv7.1 C-terminal Domain in Complex with Calmodulin disease…
3HFC 2.45 Å, A trimeric form of the Kv7.1 A domain Tail, L602M/L606M mutant Semet
7XNN 2.5 Å, human KCNQ1-CaM-ML277-PIP2 complex in state B
9UJ4 2.59 Å, Structure of human KCNQ1-CaM complex
7XNK 2.6 Å, human KCNQ1-CaM in complex with ML277
7VUO 2.68 Å, Crystal Structure of the Kv7.1 C-terminal Domain in Complex with Calmodulin disease…
9VEC 2.7 Å, structure of human KCNQ1-KCNE1-CaM complex
4V0C 2.86 Å, Crystal Structure of the Kv7.1 proximal C-terminal Domain in Complex with Calmodulin
8SIK 2.9 Å, KCNQ1 with voltage sensor in the up conformation
9U7F 2.9 Å, structure of human KCNQ1-KCNE1-CaM complex

Browse structure collections

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