5GYZ: Luciferase AMP/7-cy-L complex

luciferase AMP/7-cy-L complex. Determined by X-ray diffraction at 2.4 Å resolution. Released 27 Sept 2017.

Method: X-ray diffraction
Resolution: 2.4 Å
Organism: Photinus pyralis
Chains: 1
Atoms: 3,681
Mol. weight: 49.51 kDa
Ligands: AMP, 7BV
Released: 27 Sept 2017

Explore 5GYZ in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5GYZ contains 19 α-helices and 27 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 19 helices, 27 β-strands

Element	Residues	Length	Sheet
β-strand	8-9	2	1
α-helix	22-34	13
β-strand	40-44	5	2
β-strand	50-52	3	2
α-helix	53-70	18
β-strand	77-81	5	3
α-helix	89-97	9
β-strand	101-105	5	3
α-helix	111-121	11
β-strand	125-128	4	3
α-helix	130-132	3
α-helix	133-142	10
β-strand	148-151	4	3
β-strand	158	1	4
β-strand	161	1	4
β-strand	162-163	2	3
α-helix	164-171	8
α-helix	172-173	2
β-strand	192-197	6	3
α-helix	198	1
β-strand	207-211	5	3
α-helix	212-223	12
β-strand	236-239	4	2
α-helix	246-257	12
β-strand	261-264	4	2
α-helix	270-279	10
β-strand	284-287	4	2
α-helix	289-297	9
α-helix	301-303	3
β-strand	311-315	5	2
α-helix	321-330	10
β-strand	337-341	5	2
α-helix	343-345	3
β-strand	348-351	4	2
β-strand	363	1	1
β-strand	364-365	2	2
β-strand	370-374	5	1
α-helix	380	1
β-strand	381	1	1
α-helix	382	1
β-strand	388-393	6	1
β-strand	400-401	2	3
α-helix	405-411	7
β-strand	412	1	1
β-strand	418-426	9	1
β-strand	432-435	4	1

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Luciferin 4-monooxygenase	A	protein	435	Photinus pyralis	P08659 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>5GYZ_1 Luciferin 4-monooxygenase (chains A)
AKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVNITYAEYFEMSVRL
AEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMNISQP
TVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVP
ESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPF
HHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDK
YDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVG
KVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDI
AYWDEDEHFFIVDRL

Ligands and cofactors

ID	Name	Formula	Copies
AMP	Adenosine monophosphate	C10 H14 N5 O7 P	1
7BV	(4S)-2-[6-(azepan-1-yl)-1,3-benzothiazol-2-yl]-4,5-dihydro-1,3-thiazole-4-carbo…	C17 H19 N3 O2 S2	1

Water and common crystallization additives (PEG, GOL) are not listed.

Primary citation

Structure of luciferase with AMP/7-cy-L at 2.4 Angstroms resolution. Chao, T.Z., Su, J. To be published.

Other PDB entries of the same protein (UniProt P08659 (AlphaFold model), which also has an AlphaFold model), best resolution first:

3RIX 1.7 Å, 1.7A resolution structure of a firefly luciferase-Aspulvinone J inhibitor complex
1LCI 2.0 Å, Firefly luciferase
3IES 2.0 Å, Firefly luciferase inhibitor complex
5GZ2 2.0 Å, luciferase complex with 7-cy-L
5KYT 2.0 Å, Structure of Photinus pyralis Luciferase red light emitting variant
3IER 2.05 Å, Firefly luciferase apo structure (P41 form) with PEG 400 bound
3IEP 2.1 Å, Firefly luciferase apo structure (P41 form)
1BA3 2.2 Å, Firefly luciferase in complex with bromoform
4E5D 2.2 Å, 2.2A resolution structure of a firefly luciferase-benzothiazole inhibitor complex
5DWV 2.3 Å, Crystal structure of the Luciferase complexed with substrate analogue
4G37 2.4 Å, Structure of cross-linked firefly luciferase in second catalytic conformation
5DV9 2.4 Å, Crystal structure of the Luciferase

Browse structure collections

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