5H64: MTORC1

Cryo-EM structure of mTORC1. Determined by electron microscopy at 4.4 Å resolution. Released 25 Jan 2017.

Method: Electron microscopy
Resolution: 4.4 Å
Organism: Homo sapiens
Chains: 6
Atoms: 45,252
Mol. weight: 948.74 kDa
Released: 25 Jan 2017

Explore 5H64 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5H64 contains 350 α-helices and 166 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chains a and A: 133 helices, 15 β-strands

Element	Residues	Length	Sheet
α-helix	211-215	5
α-helix	217-221	5
α-helix	230-241	12
α-helix	248-253	6
α-helix	266-277	12
α-helix	286-293	8
α-helix	301-304	4
α-helix	308-313	6
α-helix	319-325	7
α-helix	337-340	4
α-helix	342-345	4
α-helix	356-366	11
α-helix	378-381	4
α-helix	383-388	6
α-helix	395-399	5
α-helix	414-421	8
α-helix	521-531	11
α-helix	540-547	8
α-helix	550-553	4
α-helix	559-564	6
α-helix	576-586	11
α-helix	594-603	10
α-helix	610-618	9
α-helix	620-625	6
α-helix	632-637	6
α-helix	647-655	9
α-helix	667-675	9
α-helix	683-696	14
α-helix	702-710	9
α-helix	721-730	10
α-helix	736-747	12
α-helix	749-751	3
α-helix	757-768	12
α-helix	769-772	4
α-helix	780-784	5
α-helix	795-800	6
α-helix	803-812	10
α-helix	822-835	14
α-helix	838-841	4
α-helix	844-854	11
α-helix	862-873	12
α-helix	880-883	4
α-helix	887-897	11
α-helix	902-914	13
α-helix	921-924	4
α-helix	935-940	6
α-helix	942-945	4
α-helix	948-965	18
α-helix	973-986	14
α-helix	991-993	3
α-helix	994-1006	13
α-helix	1009-1025	17
α-helix	1031-1045	15
α-helix	1050-1064	15
α-helix	1070-1073	4
α-helix	1077-1084	8
α-helix	1090-1092	3
α-helix	1093-1104	12
α-helix	1111-1120	10
α-helix	1128-1143	16
α-helix	1153-1164	12
α-helix	1168-1183	16
α-helix	1185-1188	4
α-helix	1192-1201	10
α-helix	1208-1221	14
α-helix	1256-1267	12
α-helix	1275-1290	16
α-helix	1297-1308	12
α-helix	1311-1315	5
α-helix	1318-1325	8
α-helix	1330-1348	19
α-helix	1353-1368	16
α-helix	1378-1384	7
α-helix	1394-1406	13
α-helix	1410-1422	13
α-helix	1426-1439	14
α-helix	1446-1453	8
α-helix	1456-1469	14
α-helix	1474-1486	13
α-helix	1491-1499	9
α-helix	1506-1522	17
α-helix	1527-1535	9
α-helix	1541-1553	13
α-helix	1558-1575	18
α-helix	1578-1580	3
α-helix	1586-1602	17
α-helix	1611-1614	4
α-helix	1615-1624	10
α-helix	1630-1643	14
α-helix	1646-1662	17
α-helix	1666-1677	12
β-strand	1682	1	28
β-strand	1684	1	28
α-helix	1687-1690	4
α-helix	1694-1706	13
α-helix	1711-1731	21
α-helix	1738-1762	25
α-helix	1768-1782	15
α-helix	1789-1808	20
α-helix	1868-1895	28
α-helix	1900-1910	11
α-helix	1917-1929	13
α-helix	1935-1944	10
α-helix	1945-1947	3
α-helix	1951-1964	14
α-helix	1969-1979	11
α-helix	1985-2020	36
α-helix	2023-2035	13
α-helix	2036-2040	5
α-helix	2045-2059	15
α-helix	2065-2070	6
α-helix	2071-2075	5
α-helix	2076-2091	16
α-helix	2094-2114	21
β-strand	2120-2122	3	29
α-helix	2128-2132	5
β-strand	2139	1	30
α-helix	2140	1
β-strand	2152	1	30
β-strand	2153	1	31
β-strand	2159-2162	4	29
β-strand	2170-2175	6	29
β-strand	2176	1	31
β-strand	2181-2186	6	29
α-helix	2193-2211	19
α-helix	2213-2214	2
α-helix	2215-2219	5
β-strand	2227-2229	3	29
β-strand	2235-2238	4	29
β-strand	2243-2244	2	32
α-helix	2246-2256	11
α-helix	2263-2271	9
α-helix	2275-2277	3
α-helix	2280-2292	13
α-helix	2298-2306	9
α-helix	2310-2333	24
β-strand	2344-2347	4	32
β-strand	2353-2355	3	32
α-helix	2364-2367	4
α-helix	2381-2386	6
α-helix	2392-2396	5
α-helix	2397-2422	26
α-helix	2425-2428	4
α-helix	2430-2434	5
α-helix	2494-2505	12
α-helix	2506-2510	5
α-helix	2521-2533	13
α-helix	2535-2540	6

Chains b and B: 38 helices, 32 β-strands

Element	Residues	Length	Sheet
β-strand	83-85	3	33
α-helix	99-112	14
β-strand	116-118	3	33
α-helix	125-137	13
β-strand	146-148	3	33
α-helix	162-166	5
β-strand	189-192	4	33
α-helix	199-211	13
α-helix	212-216	5
α-helix	218-220	3
β-strand	263-265	3	33
α-helix	346-350	5
α-helix	354-362	9
α-helix	367-369	3
α-helix	383-401	19
α-helix	410-419	10
α-helix	420-424	5
α-helix	449-465	17
α-helix	475-481	7
α-helix	483-485	3
α-helix	486-490	5
α-helix	500-507	8
α-helix	512-523	12
α-helix	530-536	7
α-helix	538	1
α-helix	539-543	5
α-helix	544-545	2
α-helix	586-600	15
α-helix	607-609	3
α-helix	611-620	10
α-helix	621-623	3
α-helix	627-629	3
α-helix	630-635	6
α-helix	637-642	6
α-helix	649-665	17
α-helix	668-679	12
α-helix	688-693	6
α-helix	695-703	9
α-helix	710-723	14
α-helix	730-740	11
α-helix	749-757	9
β-strand	779-782	4	34
α-helix	787	1
β-strand	788-793	6	35
β-strand	799-804	6	35
β-strand	809-813	5	35
β-strand	880-882	3	35
β-strand	892	1	36
β-strand	900-902	3	37
β-strand	903	1	36
β-strand	920-922	3	37
β-strand	927-928	2	37
β-strand	954-956	3	38
β-strand	1037-1039	3	38
β-strand	1049-1050	2	39
β-strand	1055-1056	2	39
β-strand	1085-1086	2	40
β-strand	1092-1096	5	40
α-helix	1099-1102	4
β-strand	1115-1120	6	40
β-strand	1128	1	41
β-strand	1138-1141	4	41
β-strand	1147-1152	6	41
β-strand	1156-1158	3	41
β-strand	1169-1171	3	42
β-strand	1180-1185	6	42
β-strand	1190-1194	5	42
β-strand	1215-1218	4	42
β-strand	1239	1	34
β-strand	1248-1250	3	34

Chains c and C: 4 helices, 36 β-strands

Element	Residues	Length	Sheet
β-strand	14-19	6	43
β-strand	24-27	4	43
β-strand	34-38	5	43
β-strand	48-50	3	44
β-strand	57-59	3	44
β-strand	62	1	45
β-strand	67-68	2	44
β-strand	87	1	45
β-strand	90-93	4	46
β-strand	99-103	5	46
β-strand	109-113	5	46
β-strand	123-125	3	46
α-helix	130	1
β-strand	131	1	47
β-strand	135-136	2	48
β-strand	142-145	4	48
β-strand	147	1	47
β-strand	152-156	5	48
β-strand	162-165	4	48
β-strand	173-177	5	49
β-strand	184-189	6	49
β-strand	194-199	6	49
α-helix	200	1
α-helix	203-205	3
β-strand	210-215	6	49
β-strand	226-228	3	50
β-strand	235-237	3	50
β-strand	239	1	51
β-strand	243-244	2	52
β-strand	245-246	2	50
β-strand	247	1	53
β-strand	254	1	53
β-strand	258-259	2	52
α-helix	267-269	3
β-strand	273	1	51
β-strand	276	1	54
β-strand	285-288	4	54
β-strand	294-297	4	54
β-strand	306-307	2	54
β-strand	315-321	7	43

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Serine/threonine-protein kinase mTOR	A, a	protein	2549	Homo sapiens	P42345 (AlphaFold model)
Regulatory-associated protein of mTOR	B, b	protein	1335	Homo sapiens	Q8N122 (AlphaFold model)
Target of rapamycin complex subunit LST8	C, c	protein	326	Homo sapiens	Q9BVC4 (AlphaFold model)

Sequence of entity 1 (A, a), FASTA

>5H64_1 Serine/threonine-protein kinase mTOR (chains A, a)
MLGTGPAAATTAATTSSNVSVLQQFASGLKSRNEETRAKAAKELQHYVTMELREMSQEES
TRFYDQLNHHIFELVSSSDANERKGGILAIASLIGVEGGNATRIGRFANYLRNLLPSNDP
VVMEMASKAIGRLAMAGDTFTAEYVEFEVKRALEWLGADRNEGRRHAAVLVLRELAISVP
TFFFQQVQPFFDNIFVAVWDPKQAIREGAVAALRACLILTTQREPKEMQKPQWYRHTFEE
AEKGFDETLAKEKGMNRDDRIHGALLILNELVRISSMEGERLREEMEEITQQQLVHDKYC
KDLMGFGTKPRHITPFTSFQAVQPQQSNALVGLLGYSSHQGLMGFGTSPSPAKSTLVESR
CCRDLMEEKFDQVCQWVLKCRNSKNSLIQMTILNLLPRLAAFRPSAFTDTQYLQDTMNHV
LSCVKKEKERTAAFQALGLLSVAVRSEFKVYLPRVLDIIRAALPPKDFAHKRQKAMQVDA
TVFTCISMLARAMGPGIQQDIKELLEPMLAVGLSPALTAVLYDLSRQIPQLKKDIQDGLL
KMLSLVLMHKPLRHPGMPKGLAHQLASPGLTTLPEASDVGSITLALRTLGSFEFEGHSLT
QFVRHCADHFLNSEHKEIRMEAARTCSRLLTPSIHLISGHAHVVSQTAVQVVADVLSKLL
VVGITDPDPDIRYCVLASLDERFDAHLAQAENLQALFVALNDQVFEIRELAICTVGRLSS
MNPAFVMPFLRKMLIQILTELEHSGIGRIKEQSARMLGHLVSNAPRLIRPYMEPILKALI
LKLKDPDPDPNPGVINNVLATIGELAQVSGLEMRKWVDELFIIIMDMLQDSSLLAKRQVA
LWTLGQLVASTGYVVEPYRKYPTLLEVLLNFLKTEQNQGTRREAIRVLGLLGALDPYKHK
VNIGMIDQSRDASAVSLSESKSSQDSSDYSTSEMLVNMGNLPLDEFYPAVSMVALMRIFR
DQSLSHHHTMVVQAITFIFKSLGLKCVQFLPQVMPTFLNVIRVCDGAIREFLFQQLGMLV
SFVKSHIRPYMDEIVTLMREFWVMNTSIQSTIILLIEQIVVALGGEFKLYLPQLIPHMLR
VFMHDNSPGRIVSIKLLAAIQLFGANLDDYLHLLLPPIVKLFDAPEAPLPSRKAALETVD
RLTESLDFTDYASRIIHPIVRTLDQSPELRSTAMDTLSSLVFQLGKKYQIFIPMVNKVLV
RHRINHQRYDVLICRIVKGYTLADEEEDPLIYQHRMLRSGQGDALASGPVETGPMKKLHV
STINLQKAWGAARRVSKDDWLEWLRRLSLELLKDSSSPSLRSCWALAQAYNPMARDLFNA
AFVSCWSELNEDQQDELIRSIELALTSQDIAEVTQTLLNLAEFMEHSDKGPLPLRDDNGI
VLLGERAAKCRAYAKALHYKELEFQKGPTPAILESLISINNKLQQPEAAAGVLEYAMKHF
GELEIQATWYEKLHEWEDALVAYDKKMDTNKDDPELMLGRMRCLEALGEWGQLHQQCCEK
WTLVNDETQAKMARMAAAAAWGLGQWDSMEEYTCMIPRDTHDGAFYRAVLALHQDLFSLA
QQCIDKARDLLDAELTAMAGESYSRAYGAMVSCHMLSELEEVIQYKLVPERREIIRQIWW
ERLQGCQRIVEDWQKILMVRSLVVSPHEDMRTWLKYASLCGKSGRLALAHKTLVLLLGVD
PSRQLDHPLPTVHPQVTYAYMKNMWKSARKIDAFQHMQHFVQTMQQQAQHAIATEDQQHK
QELHKLMARCFLKLGEWQLNLQGINESTIPKVLQYYSAATEHDRSWYKAWHAWAVMNFEA
VLHYKHQNQARDEKKKLRHASGANITNATTAATTAATATTTASTEGSNSESEAESTENSP
TPSPLQKKVTEDLSKTLLMYTVPAVQGFFRSISLSRGNNLQDTLRVLTLWFDYGHWPDVN
EALVEGVKAIQIDTWLQVIPQLIARIDTPRPLVGRLIHQLLTDIGRYHPQALIYPLTVAS
KSTTTARHNAANKILKNMCEHSNTLVQQAMMVSEELIRVAILWHEMWHEGLEEASRLYFG
ERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQA
WDLYYHVFRRISKQLPQLTSLELQYVSPKLLMCRDLELAVPGTYDPNQPIIRIQSIAPSL
QVITSKQRPRKLTLMGSNGHEFVFLLKGHEDLRQDERVMQLFGLVNTLLANDPTSLRKNL
SIQRYAVIPLSTNSGLIGWVPHCDTLHALIRDYREKKKILLNIEHRIMLRMAPDYDHLTL
MQKVEVFEHAVNNTAGDDLAKLLWLKSPSSEVWFDRRTNYTRSLAVMSMVGYILGLGDRH
PSNLMLDRLSGKILHIDFGDCFEVAMTREKFPEKIPFRLTRMLTNAMEVTGLDGNYRITC
HTVMEVLREHKDSVMAVLEAFVYDPLLNWRLMDTNTKGNKRSRTRTDSYSAGQSVEILDG
VELGEPAHKKTGTTVPESIHSFIGDGLVKPEALNKKAIQIINRVRDKLTGRDFSHDDTLD
VPTQVELLIKQATSHENLCQCYIGWCPFW

Sequence of entity 2 (B, b), FASTA

>5H64_2 Regulatory-associated protein of mTOR (chains B, b)
MESEMLQSPLLGLGEEDEADLTDWNLPLAFMKKRHCEKIEGSKSLAQSWRMKDRMKTVSV
ALVLCLNVGVDPPDVVKTTPCARLECWIDPLSMGPQKALETIGANLQKQYENWQPRARYK
QSLDPTVDEVKKLCTSLRRNAKEERVLFHYNGHGVPRPTVNGEVWVFNKNYTQYIPLSIY
DLQTWMGSPSIFVYDCSNAGLIVKSFKQFALQREQELEVAAINPNHPLAQMPLPPSMKNC
IQLAACEATELLPMIPDLPADLFTSCLTTPIKIALRWFCMQKCVSLVPGVTLDLIEKIPG
RLNDRRTPLGELNWIFTAITDTIAWNVLPRDLFQKLFRQDLLVASLFRNFLLAERIMRSY
NCTPVSSPRLPPTYMHAMWQAWDLAVDICLSQLPTIIEEGTAFRHSPFFAEQLTAFQVWL
TMGVENRNPPEQLPIVLQVLLSQVHRLRALDLLGRFLDLGPWAVSLALSVGIFPYVLKLL
QSSARELRPLLVFIWAKILAVDSSCQADLVKDNGHKYFLSVLADPYMPAEHRTMTAFILA
VIVNSYHTGQEACLQGNLIAICLEQLNDPHPLLRQWVAICLGRIWQNFDSARWCGVRDSA
HEKLYSLLSDPIPEVRCAAVFALGTFVGNSAERTDHSTTIDHNVAMMLAQLVSDGSPMVR
KELVVALSHLVVQYESNFCTVALQFIEEEKNYALPSPATTEGGSLTPVRDSPCTPRLRSV
SSYGNIRAVATARSLNKSLQNLSLTEESGGAVAFSPGNLSTSSSASSTLGSPENEEHILS
FETIDKMRRASSYSSLNSLIGVSFNSVYTQIWRVLLHLAADPYPEVSDVAMKVLNSIAYK
ATVNARPQRVLDTSSLTQSAPASPTNKGVHIHQAGGSPPASSTSSSSLTNDVAKQPVSRD
LPSGRPGTTGPAGAQYTPHSHQFPRTRKMFDKGPEQTADDADDAAGHKSFISATVQTGFC
DWSARYFAQPVMKIPEEHDLESQIRKEREWRFLRNSRVRRQAQQVIQKGITRLDDQIFLN
RNPGVPSVVKFHPFTPCIAVADKDSICFWDWEKGEKLDYFHNGNPRYTRVTAMEYLNGQD
CSLLLTATDDGAIRVWKNFADLEKNPEMVTAWQGLSDMLPTTRGAGMVVDWEQETGLLMS
SGDVRIVRIWDTDREMKVQDIPTGADSCVTSLSCDSHRSLIVAGLGDGSIRVYDRRMALS
ECRVMTYREHTAWVVKASLQKRPDGHIVSVSVNGDVRIFDPRMPESVNVLQIVKGLTALD
IHPQADLIACGSVNQFTAIYNSSGELINNIKYYDGFMGQRVGAISCLAFHPHWPHLAVGS
NDYYISVYSVEKRVR

Sequence of entity 3 (C, c), FASTA

>5H64_3 Target of rapamycin complex subunit LST8 (chains C, c)
MNTSPGTVGSDPVILATAGYDHTVRFWQAHSGICTRTVQHQDSQVNALEVTPDRSMIAAA
GYQHIRMYDLNSNNPNPIISYDGVNKNIASVGFHEDGRWMYTGGEDCTARIWDLRSRNLQ
CQRIFQVNAPINCVCLHPNQAELIVGDQSGAIHIWDLKTDHNEQLIPEPEVSITSAHIDP
DASYMAAVNSTGNCYVWNLTGGIGDEVTQLIPKTKIPAHTRYALQCRFSPDSTLLATCSA
DQTCKIWRTSNFSLMTELSIKSGNPGESSRGWMWGCAFSGDSQYIVTASSDNLARLWCVE
TGEIKREYGGHQKAVVCLAFNDSVLG

Primary citation

4.4 angstrom Resolution Cryo-EM structure of human mTOR Complex 1. Yang, H., Wang, J., Liu, M. et al. Protein Cell (2016) 7:878-887. DOI 10.1007/s13238-016-0346-6 · PubMed

Other PDB entries of the same protein (UniProt P42345 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4DRI 1.45 Å, Co-crystal structure of the PPIase domain of FKBP51, Rapamycin and the FRB fragment of…
9DBO 1.55 Å, Crystal structure of a synthetic Fab (R3E9) in complex with the FRB domain of mTOR
5GPG 1.67 Å, Co-crystal structure of the FK506 binding domain of human FKBP25, Rapamycin and the FRB…
5WBH 1.75 Å, Structure of the FRB domain of mTOR bound to a substrate recruitment peptide of S6K1
4DRJ 1.8 Å, o-crystal structure of the PPIase domain of FKBP52, Rapamycin and the FRB fragment of mTOR
3FAP 1.85 Å, Atomic structures of the rapamycin analogs in complex with both human FKBP12 and frb…
8PPZ 1.85 Å, Co-crystal structure of FKBP12, compound 7 and the FRB fragment of mTOR
8XI9 1.85 Å, Crystal structure of FRB-FKBP fusion protein in complex with rapamycin
9PIW 1.9 Å, Crystal structure of a synthetic Fab (1A) in complex with the FRB domain of mTOR
9DL0 2.0 Å, Crystal structure of a synthetic Fab (R3H8) in complex with the FRB domain of mTOR
9PJ1 2.05 Å, Crystal structure of a synthetic Fab (4R) in complex with the ternary assembly of…
9PIU 2.18 Å, Crystal structure of a synthetic Fab (2C) in complex with the FRB domain of mTOR

Browse structure collections

About this viewer

MolViewer shows 5H64 directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.