5I1P: Villin-1
Villin headpiece subdomain with a Lys30 to beta-3-homolysine substitution. Determined by X-ray diffraction at 1.4 Å resolution. Released 25 May 2016.
- Method
- X-ray diffraction
- Resolution
- 1.4 Å
- Organisms
- Gallus gallus, synthetic construct
- Chains
- 8
- Atoms
- 2,449
- Mol. weight
- 32.88 kDa
- Ligands
- EOH
- Released
- 25 May 2016
Explore 5I1P in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
5I1P contains 24 α-helices and 0 β-strands across 8 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chains A and G: 3 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 3-10 | 8 | |
| α-helix | 14-18 | 5 | |
| α-helix | 22-32 | 11 | |
Chains B and E: 3 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 3-10 | 8 | |
| α-helix | 14-17 | 4 | |
| α-helix | 22-31 | 10 | |
Chain C: 3 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 3-10 | 8 | |
| α-helix | 14-17 | 4 | |
| α-helix | 22-30 | 9 | |
Chains D and F: 3 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 3-10 | 8 | |
| α-helix | 14-18 | 5 | |
| α-helix | 22-31 | 10 | |
Chain H: 3 helices, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 3-10 | 8 | |
| α-helix | 14-17 | 4 | |
| α-helix | 22-32 | 11 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Villin-1 | A, B, C, D | protein | 35 | Gallus gallus | P02640 (AlphaFold model) |
| D-Villin headpiece subdomain | E, F, G, H | protein | 35 | synthetic construct | |
Sequence of entity 1 (A, B, C, D), FASTA
>5I1P_1 Villin-1 (chains A, B, C, D)
LSDEDFKAVFGMTRSAFANLPLWKQQHLKKEKGLF
Sequence of entity 2 (E, F, G, H), FASTA
>5I1P_2 D-Villin headpiece subdomain (chains E, F, G, H)
LSDEDFKAVFGMTRSAFANLPLWKQQHLKKEKGLF
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| EOH | Ethanol | C2 H6 O | 3 |
Primary citation
Effects of Single alpha-to-beta Residue Replacements on Structure and Stability in a Small Protein: Insights from Quasiracemic Crystallization. Kreitler, D.F., Mortenson, D.E., Forest, K.T. et al. J Am Chem Soc (2016) 138:6498-6505. DOI 10.1021/jacs.6b01454 · PubMed
Other PDB entries of the same protein (UniProt P02640 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 1WY3 0.95 Å, Chicken villin subdomain HP-35, K65(NLE), N68H, pH7.0
- 1YRI 1.0 Å, Chicken villin subdomain HP-35, N68H, pH6.4
- 3TRV 1.0 Å, Crystal structure of quasiracemic villin headpiece subdomain containing (F5Phe17)…
- 2F4K 1.05 Å, Chicken villin subdomain HP-35, K65(NLE), N68H, K70(NLE), PH9
- 1YRF 1.07 Å, Chicken villin subdomain HP-35, N68H, pH6.7
- 5I1S 1.12 Å, Villin headpiece subdomain with a Lys30 to APC substitution
- 5I1N 1.3 Å, Villin headpiece subdomain with a Gln26 to beta-3-homoglutamine substitution
- 5I1O 1.35 Å, Villin headpiece subdomain with a Gln26 to ACPC substitution
- 1YU5 1.4 Å, Crystal Structure of the Headpiece Domain of Chicken Villin
- 2RJY 1.4 Å, Crystal structure of villin headpiece, P21 21 21 space group
- 1YU8 1.45 Å, Crystal Structure of the R37A Mutant of Villin Headpiece
- 2RJV 1.45 Å, Crystal structure of the H41Y mutant of villin headpiece, P 21 21 21 space group
Browse structure collections
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