5I1S: Villin-1

Villin headpiece subdomain with a Lys30 to APC substitution. Determined by X-ray diffraction at 1.12 Å resolution. Released 25 May 2016.

Method: X-ray diffraction
Resolution: 1.12 Å
Organisms: Gallus gallus, synthetic construct
Chains: 4
Atoms: 1,510
Mol. weight: 16.31 kDa
Released: 25 May 2016

Explore 5I1S in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5I1S contains 12 α-helices and 0 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chains A and D: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	3-10	8
α-helix	14-18	5
α-helix	22-31	10

Chains B and C: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	3-10	8
α-helix	14-19	6
α-helix	22-31	10

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Villin-1	A, B	protein	35	Gallus gallus	P02640 (AlphaFold model)
D-Villin headpiece subdomain	C, D	protein	35	synthetic construct

Sequence of entity 1 (A, B), FASTA

>5I1S_1 Villin-1 (chains A, B)
LSDEDFKAVFGMTRSAFANLPLWKQQHLKXEKGLF

Sequence of entity 2 (C, D), FASTA

>5I1S_2 D-Villin headpiece subdomain (chains C, D)
LSDEDFKAVFGMTRSAFANLPLWKQQHLKKEKGLF

Primary citation

Effects of Single alpha-to-beta Residue Replacements on Structure and Stability in a Small Protein: Insights from Quasiracemic Crystallization. Kreitler, D.F., Mortenson, D.E., Forest, K.T. et al. J Am Chem Soc (2016) 138:6498-6505. DOI 10.1021/jacs.6b01454 · PubMed

Other PDB entries of the same protein (UniProt P02640 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1WY3 0.95 Å, Chicken villin subdomain HP-35, K65(NLE), N68H, pH7.0
1YRI 1.0 Å, Chicken villin subdomain HP-35, N68H, pH6.4
3TRV 1.0 Å, Crystal structure of quasiracemic villin headpiece subdomain containing (F5Phe17)…
2F4K 1.05 Å, Chicken villin subdomain HP-35, K65(NLE), N68H, K70(NLE), PH9
1YRF 1.07 Å, Chicken villin subdomain HP-35, N68H, pH6.7
5I1N 1.3 Å, Villin headpiece subdomain with a Gln26 to beta-3-homoglutamine substitution
5I1O 1.35 Å, Villin headpiece subdomain with a Gln26 to ACPC substitution
1YU5 1.4 Å, Crystal Structure of the Headpiece Domain of Chicken Villin
2RJY 1.4 Å, Crystal structure of villin headpiece, P21 21 21 space group
5I1P 1.4 Å, Villin headpiece subdomain with a Lys30 to beta-3-homolysine substitution
1YU8 1.45 Å, Crystal Structure of the R37A Mutant of Villin Headpiece
2RJV 1.45 Å, Crystal structure of the H41Y mutant of villin headpiece, P 21 21 21 space group

Browse structure collections

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