5J03: PDB entry 5J03

Crystal Structure of a chimeric Kv7.2 - Kv7.3 proximal C-terminal Domain in Complex with Calmodulin. Determined by X-ray diffraction at 2.0 Å resolution. Released 7 Sept 2016.

Method: X-ray diffraction
Resolution: 2.0 Å
Organism: Homo sapiens
Chains: 2
Atoms: 1,871
Mol. weight: 30.18 kDa
Ligands: CA
Released: 7 Sept 2016

Explore 5J03 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5J03 contains 13 α-helices and 4 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	352-375	24
α-helix	386-392	7
α-helix	535-556	22

Chain B: 10 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	7-20	14
β-strand	27-28	2	1
α-helix	30-39	10
α-helix	46-56	11
β-strand	64-65	2	1
α-helix	66-73	8
α-helix	77-80	4
α-helix	83-93	11
β-strand	100-102	3	2
α-helix	103-112	10
α-helix	116-118	3
α-helix	119-129	11
β-strand	136-138	3	2
α-helix	139-147	9

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Potassium voltage-gated channel subfamily KQT member 3,Potassium voltage-gated channel subfamily…	A	protein	110	Homo sapiens	O43525 (AlphaFold model)
Calmodulin	B	protein	149	Homo sapiens	P0DP23 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>5J03_1 Potassium voltage-gated channel subfamily KQT member 3,Potassium voltage-gated channel subfamily KQT member 2 (chains A)
MGSHHHHHHHHGSDYDIPTTENLYFQGSALKVQEQHRQKHFEKRRKPAAELIQAAWRYYA
TNPNRIDLVATWRFYESVVSFPEDLTPGLKVSIRAVCVMRFLVSKRKFKE

Sequence of entity 2 (B), FASTA

>5J03_2 Calmodulin (chains B)
MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADG
NGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDE
EVDEMIREADIDGDGQVNYEEFVQMMTAK

Ligands and cofactors

ID	Name	Formula	Copies
CA	Calcium ion	Ca	5

Water and common crystallization additives (ACT) are not listed.

Primary citation

Structural Insights into the M-Channel Proximal C-Terminus/Calmodulin Complex. Strulovich, R., Tobelaim, W.S., Attali, B. et al. Biochemistry (2016) 55:5353-5365. DOI 10.1021/acs.biochem.6b00477 · PubMed

Other PDB entries of the same protein (UniProt O43525 (AlphaFold model), which also has an AlphaFold model), best resolution first:

22BJ 2.4 Å, KCNQ2/3 heterotetramer with 3:1 stoichiometry
22BF 2.5 Å, XEN1101 bound KCNQ2/3 heteromer with 3:1 stoichiometry, state 2
22BG 2.5 Å, XEN1101 bound KCNQ2/3 heteromer with 3:1 stoichiometry, state 3
22BE 2.6 Å, XEN1101 bound KCNQ2/3 heteromer with 3:1 stoichiometry, state 1
22BI 2.6 Å, XEN1101 bound KCNQ2/3 heteromer with 2:2 stoichiometry
22AZ 2.7 Å, ICA-1103811 bound KCNQ2/3 heteromer with 3:1 stoichiometry, state 1
22BK 2.7 Å, KCNQ2/3 heterotetramer with 2:2 stoichiometry
22BC 2.8 Å, ICA-1103811 bound KCNQ2/3 heteromer with 3:1 stoichiometry, state 3
22BH 2.8 Å, XEN1101 bound KCNQ2/3 heteromer with 3:1 stoichiometry, state 4
22RJ 2.82 Å, Human KCNQ3-CaM in apo state
22BA 2.9 Å, ICA-1103811 bound KCNQ2/3 heteromer with 3:1 stoichiometry, state 2
22RQ 2.9 Å, Human KCNQ3-XEN1101 complex in the presence of PIP2

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