5LT1: Kinesin-like protein

nucleotide-free kinesin-1 motor domain T92V mutant, P21 crystal form. Determined by X-ray diffraction at 1.95 Å resolution. Released 1 Mar 2017.

Method: X-ray diffraction
Resolution: 1.95 Å
Organism: Homo sapiens
Chains: 2
Atoms: 5,490
Mol. weight: 73.16 kDa
Ligands: SR
Released: 1 Mar 2017

Explore 5LT1 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5LT1 contains 32 α-helices and 38 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 15 helices, 19 β-strands

Element	Residues	Length	Sheet
β-strand	9-15	7	1
α-helix	16-18	3
α-helix	20-25	6
β-strand	29	1	2
β-strand	32-34	3	3
β-strand	38-41	4	3
β-strand	44-47	4	3
β-strand	50-52	3	1
α-helix	58-65	8
α-helix	67-74	8
β-strand	79-84	6	1
α-helix	91-95	5
β-strand	97	1	4
β-strand	105	1	4
α-helix	107-121	15
β-strand	126-138	13	1
β-strand	141-144	4	1
β-strand	153	1	1
α-helix	154	1
β-strand	155-157	3	5
β-strand	163-165	3	5
β-strand	171-172	2	1
α-helix	176-189	14
α-helix	197-202	6
β-strand	205-216	12	1
β-strand	221-231	11	1
α-helix	232-234	3
α-helix	249-269	21
α-helix	277-279	3
α-helix	281-285	5
β-strand	295-302	8	1
β-strand	305	1	2
α-helix	306-308	3
α-helix	309-320	12

Chain B: 17 helices, 19 β-strands

Element	Residues	Length	Sheet
α-helix	8-9	2
β-strand	10-15	6	6
α-helix	16-19	4
α-helix	20-25	6
β-strand	29	1	7
β-strand	32-34	3	8
β-strand	38-41	4	8
β-strand	44-47	4	8
β-strand	50-52	3	6
α-helix	58-65	8
α-helix	67-73	7
β-strand	79-84	6	6
α-helix	91-95	5
β-strand	97	1	9
β-strand	105	1	9
α-helix	107-121	15
β-strand	126-138	13	6
β-strand	141-144	4	6
β-strand	153	1	6
α-helix	154	1
β-strand	155-157	3	10
β-strand	163-165	3	10
β-strand	171-173	3	6
α-helix	176-189	14
α-helix	197-202	6
β-strand	205-216	12	6
β-strand	222-231	10	6
α-helix	232-234	3
α-helix	249-269	21
α-helix	277-279	3
α-helix	281-285	5
α-helix	287-289	3
β-strand	295-302	8	6
β-strand	305	1	7
α-helix	306-308	3
α-helix	309-320	12

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Kinesin-like protein	A, B	protein	325	Homo sapiens	P33176 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>5LT1_1 Kinesin-like protein (chains A, B)
MADLAESNIKVMCRFRPLNESEVNRGDKYIAKFQGEDTVVIASKPYAFDRVFQSSTSQEQ
VYNDAAKKIVKDVLEGYNGTIFAYGQTSSGKVHTMEGKLHDPEGMGIIPRIVQDIFNYIY
SMDENLEFHIKVSYFEIYLDKIRDLLDVSKTNLSVHEDKNRVPYVKGATERFVSSPDEVM
DTIDEGKSNRHVAVTNMNEHSSRSHSIFLINVKQENTQTEQKLSGKLYLVDLAGSEKVSK
TGAEGAVLDEAKNINKSLSALGNVISALAEGSTYVPYRDSKMTRILQDSLGGNARTTIVI
CCSPSSYNESETKSTLLFGQRAKTI

Ligands and cofactors

ID	Name	Formula	Copies
SR	Strontium ion	Sr	1

Water and common crystallization additives (SO4, ACT) are not listed.

Primary citation

The structural switch of nucleotide-free kinesin. Cao, L., Cantos-Fernandes, S., Gigant, B. Sci Rep (2017) 7:42558-42558. DOI 10.1038/srep42558 · PubMed

Other PDB entries of the same protein (UniProt P33176 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1BG2 1.8 Å, Human ubiquitous kinesin motor domain
5LT0 2.0 Å, nucleotide-free kinesin-1 motor domain, P212121 crystal form
4LNU 2.19 Å, Nucleotide-free kinesin motor domain in complex with tubulin and a DARPin
9L7E 2.4 Å, Crystal structure of human kinesin-1 motor domain (G234A mutant) in complex with ADP
5LT3 2.59 Å, nucleotide-free kinesin-1 motor domain T87A mutant, P1 crystal form
5LT2 2.6 Å, nucleotide-free kinesin-1 motor domain, P1 crystal form
1MKJ 2.7 Å, Human Kinesin Motor Domain With Docked Neck Linker
9L6K 2.8 Å, Crystal structure of nucleotide-free human kinesin-1 motor domain (G234V mutant)
9L78 2.82 Å, Crystal structure of nucleotide-free human kinesin-1 motor domain (G234A mutant)
5LT4 2.88 Å, nucleotide-free kinesin-1 motor domain T92V mutant, P1 crystal form
9GNQ 2.9 Å, Microtubule-associated Kif5B IAK tail mutant bound to ADP
7TR2 3.0 Å, Apo CaKip3[2-436]-L2-mutant(HsKHC) in complex with a microtubule

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