5NVL: Human 4EHP-GIGYF2 complex

Crystal structure of the human 4EHP-GIGYF2 complex. Determined by X-ray diffraction at 2.3 Å resolution. Released 26 Jul 2017.

Method: X-ray diffraction
Resolution: 2.3 Å
Organism: Homo sapiens
Chains: 4
Atoms: 3,811
Mol. weight: 61.34 kDa
Released: 26 Jul 2017

Explore 5NVL in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5NVL contains 28 α-helices and 20 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 6 helices, 8 β-strands

Element	Residues	Length	Sheet
β-strand	55-66	12	1
β-strand	83-90	8	1
α-helix	91-98	8
β-strand	101	1	2
α-helix	104-106	3
β-strand	111-117	7	1
α-helix	127-130	4
β-strand	133-139	7	1
α-helix	140	1
α-helix	144-156	13
β-strand	166-173	8	1
β-strand	178-184	7	1
α-helix	190-204	15
β-strand	213-215	3	1

Chain B: 9 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	45-50	6
α-helix	57-59	3
α-helix	60-63	4
α-helix	65-70	6
β-strand	71	1	2
α-helix	75-78	4
α-helix	79-81	3
α-helix	83-84	2
α-helix	86-94	9
β-strand	97	1	1
α-helix	99-102	4

Chain C: 5 helices, 8 β-strands

Element	Residues	Length	Sheet
β-strand	55-66	12	3
β-strand	82-90	9	3
α-helix	91-98	8
β-strand	101	1	4
α-helix	104-106	3
β-strand	111-117	7	3
α-helix	127-129	3
β-strand	133-139	7	3
α-helix	144-156	13
β-strand	166-173	8	3
β-strand	178-184	7	3
α-helix	190-204	15
β-strand	213-216	4	3

Chain D: 8 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	45-50	6
α-helix	57-59	3
α-helix	60-63	4
α-helix	65-70	6
β-strand	71	1	4
α-helix	75-78	4
α-helix	79-81	3
α-helix	83-85	3
α-helix	86-93	8
β-strand	97	1	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Eukaryotic translation initiation factor 4E type 2	A, C	protein	189	Homo sapiens	O60573 (AlphaFold model)
GRB10-interacting GYF protein 2	B, D	protein	75	Homo sapiens	Q6Y7W6 (AlphaFold model)

Sequence of entity 1 (A, C), FASTA

>5NVL_1 Eukaryotic translation initiation factor 4E type 2 (chains A, C)
GPHMLEAEHPLQYNYTFWYSRRTPGRPTSSQSYEQNIKQIGTFASVEQFWRFYSHMVRPG
DLTGHSDFHLFKEGIKPMWEDDANKNGGKWIIRLRKGLASRCWENLILAMLGEQFMVGEE
ICGAVVSVRFQEDIISIWNKTASDQATTARIRDTLRRVLNLPPNTIMEYKTHTDSIKMPG
RLGPQRLLF

Sequence of entity 2 (B, D), FASTA

>5NVL_2 GRB10-interacting GYF protein 2 (chains B, D)
GPHMKYKLADYRYGREEMLALFLKDNKIPSDLLDKEFLPILQEEPLPPLALVPFTEEEQR
NFSMSVNSAAVLRLT

Primary citation

GIGYF1/2 proteins use auxiliary sequences to selectively bind to 4EHP and repress target mRNA expression. Peter, D., Weber, R., Sandmeir, F. et al. Genes Dev (2017) 31:1147-1161. DOI 10.1101/gad.299420.117 · PubMed

Other PDB entries of the same protein (UniProt O60573 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2JGB 1.7 Å, Structure of human eIF4E homologous protein 4EHP with m7GTP
5NVN 1.9 Å, Crystal structure of the human 4EHP-4E-BP1 complex
5XLN 1.9 Å, Crystal structure of the TRS_UNE-T and 4EHP complex
5NVM 2.0 Å, Crystal structure of the human 4EHP-GIGYF2 complex lacking the auxiliary sequences
2JGC 2.4 Å, Structure of the human eIF4E homologous protein, 4EHP without ligand bound
5NVK 2.9 Å, Crystal structure of the human 4EHP-GIGYF1 complex

Browse structure collections

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